Effects of titanium dioxide nanoparticle exposure in Mytilus galloprovincialis gills and digestive gland

Abstract

Despite the wide use of nanoscale materials in several fields, some aspects of the nanoparticle behavior have to be still investigated. In this work, we faced the aspect of environmental effects of increasing concentrations of TiO₂NPs using the Mytilus galloprovincialis as an animal model and carrying out a multidisciplinary approach to better explain the results. Bioaccumulation suggested that the gills and digestive gland are the most sensitive organs to TiO₂NP exposure. Histological observations have evidenced an altered tissue organization and a consistent infiltration of hemocytes, as a consequence of the immune system activation, even though an increase in lipid peroxidation is uncertain and DNA damage became relevant only at high exposure dose (10 mg/L) or for longer exposure time (96 h). However, the over expression of SOD1 mRNA strengthen the concept that the toxicity of TiO₂NPs could occur indirectly by ROS production. TEM analysis showed the presence of multilamellar bodies, RER fragmentation, and cytoplasmic vacuolization within relevant presence of dense granules, residual bodies, and lipid inclusions. These findings support the evidence of an initial inflammatory response by the cells of the target organs leading to apoptosis. In conclusion, we can state that certainly the exposure to TiO₂NPs has affected our animal model from cellular to molecular levels. Interestingly, the same responses are caused by lower TiO₂NP concentration and longer exposure time as well as higher doses and shorter exposure. We do not know if some of the conditions detected are reversible, then further studies are required to clarify this aspect.

• Biomarkers
• in vivo experiments
• morphology
• nanotoxicity

Declaration of interest

This research was supported by PRIN grant (Prot. 2010ARBLT7_001/008) to S. F. and R. G. and by FAR (Fondo comune di Ateneo per la Ricerca 2013) to R. G. and G. B.

Supplementary material available online