Development of a multiplex amplification refractory mutation system for simultaneous authentication of Korean ginseng cultivars “Gumpoong” and “Chungsun”

Abstract

Background and aims. Molecular authentication of Korean ginseng cultivars was investigated using the mitochondrial nicotinamide adenine dinucleotide (NADH) dehydrogenase subunit 7 (nad7) intron 3 region. Materials and methods. A mutation site specific to Panax ginseng “Gumpoong” and “Chungsun” cultivars was detected within the sequence data. Based on this mutation site and the “Gumpoong”-specific single nucleotide polymorphism site reported in 26S rDNA, two modified allele-specific primer pairs were designed and a multiplex amplification refractory mutation system (MARMS) was applied to identify “Gumpoong” and “Chungsun.” Results. The results showed that “Gumpoong” and “Chungsun” can be clearly discriminated from the other Korean ginseng cultivars by simultaneously identifying the haplotype of “Gumpoong” and the specific allele of “Chungsun” by applying the MARMS. Conclusion. This study, therefore, provides a simple and reliable method for simultaneous authentication of “Gumpoong” and “Chungsun” cultivars.

Keywords::

• Panax ginseng
• Gumpoong
• Chungsun
• cultivar
• nad7
• multiplex amplification refractory mutation system

Acknowledgements

This study was supported by the Korean Ginseng Center for Most Valuable Products, Technology Development Program of Agriculture and Forestry, Ministry of Agriculture and Forestry, Republic of Korea.

Declaration of interest: The authors report no conflicts of interest. The authors alone are responsible for the content writing of the paper.