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Abstract
Liriomyza huidobrensis, L. trifolii and L. sativae (Diptera: Agromyzidae) are serious horticultural pests. The three species are very similar and difficult to discriminate from each other. In this study, we report the mitochondrial genome of L. huidobrensis and compare with L. trifolii and L. sativae. The mitochondrial genome of L. huidobrensis is 16,239 bp long, and it contains 37 genes and one A + T-rich region as do the sequenced liriomyza species. The sequence and structure of H51-H100 region in srRNA is significantly different from that of L. trifolii and L. sativae. The greatest sequence differences among the three leafminers are located in the region including ND2, ND3, ND6, ATP6, ATP8, CYTb and A+T region, which can be considered as candidate regions for molecular identification or SNP markers.