Abstract
The hybrid between Nile tilapia (Oreochromis niloticus,♀) and blue tilapia (O. aureus,♂) is an important strain in Chinese aquaculture industry. Two populations named AF (O. aureus, 29 samples) and NF (O. niloticus, 22 samples) were gathered from Freshwater Fisheries Research Center (FFRC). The other two named AG (O. aureus, 29 samples) and NG (O. niloticus, 28 samples) from Guangxi Fisheries Research Institute (GFRI). GFRI introduced AG and NG from AF and NF. The mitochondrial DNA D-loop was sequenced to assess the genetic diversity among four populations. A 580 bp fragment was sequenced. The 93 variable sites defined 39 haplotypes and three were shared. As a result, the genetic diversity of O. aureus AF and AG was much lower (H = 0.497–0.532, K = 0.69–0.714, π = 0.0012–0.00124) than that of O. niloticus (H = 0.849–0.866, K = 24.286–24.807, π = 0.04246–0.04337). Furthermore, the indices (H, K, π and D) was a slight increase between AF and AG, so did NF and NG. These results indicated that as the male parent, the AF and AG population was purebred and sustainable. And as the female parent, NF and NG had high genetic diversity. The conclusions might give reference to keep the germplasm diversity of tilapia and other introduced fishes.
Acknowledgements
The authors express our special thanks to Mr. Xi Gan, the director of GFRI (China), as well as Liping Li, Ming Chen, Lan Zeng et al. in the sampling process which give supports and helps.
Declaration of interest
No conflict of interest exits in the submission of this manuscript, and manuscript is approved by all authors for publication. I would like to declare on behalf of my co-authors that the work described was original research that has not been published previously, and not under consideration for publication elsewhere, in whole or in part. All the authors listed have approved the manuscript that is enclosed. This research was supported by the earmarked fund for Modern Agro-industry Technology Research System (nycytx-48), China Agriculture Technique System (CARS-49), National Basic R&D Program of China (2011JBFA04), Modern Agriculture Personnel Support Program and the Tilapia Original Breeding (2130135).