Abstract
We sequenced 17,329 bp of the black dwarf honey bee, Apis andreniformis (Hymenoptera: Apidae) mitochondrial genome (mitogenome) that lacked ∼200 bp of the A + T-rich region for the complete genomic sequence. The gene arrangement of the A. andreniformis mitogenome was identical to that of A. cerana. However, the genome contained five additional tRNALeu(CUN); four copies were located between tRNAMet and tRNAGln, and one copy was between tRNAGln and tRNAAla, along with the typical sets of genes (13 protein-coding genes, 22 tRNAs, and 2 rRNAs) including regular tRNALeu(CUN) and the A + T-rich region (at least 923 bp). Only one copy of tRNALeu(CUN) differed by 1 bp from the other four copies of tRNALeu(CUN). Each additional tRNALeu(CUN) was followed by a nearly identical 68-bp long repeat sequence (95.6% identity). All 13 protein coding genes had typica start codons found in insect mitochondrial protein coding genes (two ATA, nine ATT and two ATG).
Declaration of interest
The authors report no conflicts of interest. The authors alone are responsible for the content and writing of this manuscript. This work was supported by a grant from the Next-Generation BioGreen 21 Program (no. PJ009031), Rural Development Administration, Republic of Korea.