Molecular characterization of the Cenxi classical three-buff chicken (Gallus gallus domesticus) based on mitochondrial DNA

Abstract

The complete mitochondrial genome sequence of the Cenxi classical three-buff chicken was measured by PCR-based methods and analyzed in detail. Our research findings reveal that the entire mitochondrial genome of the Cenxi classical three-buff chicken is a circular molecule consisting of 16,786 bp (GenBank accession number: KM433666). The contents of A, T, C, and G in the mitochondrial genome were found to be 30.27%, 23.75%, 32.49% and 13.49%, respectively. The complete mitochondrial genome of the Cenxi classical three-buff chicken contains a typical structure, including 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and 1 control region (D-loop region). This complete mitochondrial genome sequence provides essential information in understanding phylogenetic relationships among Gallus gallus domesticus mitochondrial genomes.

Keywords:

• Cenxi classical three-buff chicken
• genome organization
• mitochondrial genome

Acknowledgements

The authors gratefully acknowledge Dr. Huaguang Lu (The Pennsylvania State University, USA) for providing excellent suggestions and detail review on this manuscript, and his internship students Eric Knoll and Jharmaine Aguila from the Pennsylvania State University for assisting this research experiment during their 2014 international summer internship studies at our lab.

Declaration of interest

The authors declare no conflicts of interest. The authors are responsible for the content and writing of the article. This research project was funded by The Guangxi Science and Technology Bureau (Grant No.1222003-2-4 and 10100014-5) and The Guangxi Government Senior Scientist Foundation (Grant No.2011B020).