Abstract
The complete mitochondrial genome of Temnopleurus hardwickii was sequenced using long-PCR and primer walking methods. The complete mitochondrial genome is a 15,696 bp circular molecule which contains 22 tRNAs, 2 rRNAs, 13 protein-coding genes as well as a non-coding control region as in other urchin. The genome was composed of 32.9% A, 29.3% T, 21.4% C and 16.4% G, showing a slight AT bias (62.3%). The control region is 156 bp. Twelve of the 13 mitochondrial protein-coding genes use ATG as their start codon while the ATP8 gene starts with GTG. All the 13 mitochondrial protein-coding genes use TAG or TAA as their complete stop codon.
Acknowledgements
We are grateful to Dr Shichun Sun and Sijia Xu for the sample collection.
Declaration of interest
This study was Supported by the Fundamental Research Funds for the Central Universities (201362023, 201262037). The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.