PCR-free shotgun sequencing of the stone loach mitochondrial genome (Barbatula barbatula)

Abstract

The complete mitochondrial genome of the stone loach Barbatula barbatula (Linnaeus, 1758) (Actinopterygii: Cypriniformes: Nemacheilidae) has been sequenced using a genome-skimming approach on an Illumina Hiseq 2500 platform. The mitochondrial genome of B. barbatula was determined to be 16,630 bp long and presents an organization typical of vertebrate mitogenomes. The mean coverage was 82× with a minimum coverage of 33× for the control region and 52× for the remaining part of the genome. A phylogenetic analysis of the Nemacheilidae family shows the monophyly of the Barbatula genus with strong support.

Keywords:

• Fish
• genome skimming
• illumina
• next-generation sequencing

Declaration of interest

This work was supported by “Investissement d’Avenir” grants managed by Agence Nationale de la Recherche (CEBA, ref. ANR-10-LABX-25-01; TULIP, ANR-10-LABX-41, and ANR-11-IDEX-0002-02) as well as project METABAR (ANR-11-BSV7-0020). The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.