Complete mitochondrial genome of Lutzomyia (Nyssomyia) umbratilis (Diptera: Psychodidae), the main vector of Leishmania guyanensis

Abstract

The nearly complete mitochondrial genome of Lutzomyia umbratilis Ward & Fraiha, 1977 (Psychodidae: Phlebotominae), considered as the main vector of Leishmania guyanensis, is presented. The sequencing has been performed on an Illumina Hiseq 2500 platform, with a genome skimming strategy. The full nuclear ribosomal RNA segment was also assembled. The mitogenome of L. umbratilis was determined to be at least 15,717 bp-long and presents an architecture found in many mitogenomes of insect (13 protein-coding genes, 22 transfer RNAs, two ribosomal RNAs, and one non-coding region also referred as the control region). The control region contains a large repeated element of c. 370 bp and a poly-AT region of unknown length. This is the first mitogenome of Psychodidae to be described.

Keywords:

• Genome skimming
• illumina Hiseq
• leishmaniasis
• next-generation sequencing
• Sandfly
• shotgun sequencing

Acknowledgements

We would like to thank Romuald Carinci and Jean Issaly for their involvement in the collection and processing of the samples.

Declaration of interest

This work was supported by “Investissement d’Avenir” grants managed by Agence Nationale de la Recherche (CEBA, ANR-10-LABX-25-01; TULIP, ANR-10-LABX-41, and ANR-11-IDEX-0002-02) as well as project METABAR (ANR-11-BSV7-0020). The collection of sandflies was performed in the framework of the French National Project ANR 06- SEST-20 IAEL. The authors report that they have no conflicts of interest. The authors alone are responsible for the content and writing of the paper.

Supplementary material available online