Sequencing and analysis of the complete mitochondrial genome in Anopheles culicifacies species B (Diptera: Culicidae)

Abstract

The complete mitochondrial genome sequence of Anopheles culicifacial species B was sequenced in this study. The length of the mitochondrial genome is 15 330 bp, which contains 13 protein-coding genes (PCGs), 22 tRNA genes, two rRNA genes, and a non-coding control region. The gene order and the gene composition are consistent with those previously reported for other mosquito species. The initiation codon of the PCGs complies with the ATN rule except for COI using TCG and ND5 using GTG as a start codon, and the termination codon is TAA or imcomplete, an only T. The total base composition is 40.4% A, 38.1% T, 12.4% C, and 9.1% G. The phylogenetic tree based on the sequences of 13 protein-coding genes showed that these species were classified into two clades, corresponding to the subgenus Cellia and subgenus Nyssorhynchus. An. culicifacies species B of Myzomyia Series was clustered with An. gambiae of Pyretophorus Series with a high bootstrap value of 100%. The complete mitogenome data can provide a basis for molecular identification and phylogenetic studies of mosquito species.

• Anopheles culicifacies species B
• Culicidae
• mitochondrial genome
• phylogenetic tree
• sequencing analysis

Declaration of interest

The authors declare no conflicts of interest. The authors alone are responsible for the content and writing of the paper. This work was supported by Par-Eu Scholars Program, and grants from The National Natural Science Foundation of China (31372265), Coordinated Research Project of the International Atomic Energy Agency (18268/R1), National Institute of Health (R01 AI095184) and the Youth Foundation of Chongqing Normal University (13XLQ05, 2011XLS32).