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Mitochondrial DNA Part A
DNA Mapping, Sequencing, and Analysis
Volume 27, 2016 - Issue 6
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Mitogenome Announcement

Complete mitochondrial genome of the soybean stem fly Melanagromyza sojae (Diptera: Agromyzidae)

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Pages 4534-4535 | Received 30 Aug 2015, Accepted 09 Sep 2015, Published online: 22 Mar 2016
 

Abstract

We report the complete mitochondrial DNA genome of the soybean stem fly (SSF) Melanagromyza sojae from Brazil Santa Catarina state based on Illumina MiSeq sequence data. The estimated mitogenome is 15 475 base pairs (bp) (KT597923), with 13 protein-coding genes (PCGs), two ribosomal RNA (rRNA) genes, and 22 tRNAs, and an estimated 579 bp AT-rich control region. Similar to other insects, the SSF mitogenome is A–T bias with 40.9% A, 36.7% T, 13.6% C, and 8.8% G. Molecular characterization of SSF mitogenome will facilitate the development of effective molecular markers, and robust and rapid identification of suspected biosecurity incursions and field infestations of this insect pest.

Acknowledgements

The authors thank Mr. William James and Ms. Melissa Piper (CSIRO) for their technical assistance in the laboratory. J. A. A. acknowledged the CSIRO Health and Biosecurity Flagship for hosting his visit to enable completion of molecular genetic laboratory component of this study.

Declaration of interest

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of this article.

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