The complete mitochondrial genome of the king horseshoe bat (Rhinolophus rex) using next-generation sequencing and Sanger sequencing

Abstract

The king horseshoe bat (Rhinolophus rex) is endemic to China. Here, we characterized the complete mitochondrial genome of R. rex using next-generation sequencing and Sanger sequencing. The mitogenome is 16 845 bp in length and contains 22 tRNAs, two rRNAs, 13 protein-coding genes, and a non-coding control region. Our traditional Sanger sequencing verified that mtDNA sequences generated from next-generation sequencing are reliable. The mitogenome of R. rex will be useful for the conservation genetics of this endangered species.

Keywords:

• Bat
• de novo assembly
• mitogenome
• Rhinolophus rex

Acknowledgements

The authors thank Zhang Junpeng, Zhu Guangjian, and Meng Fanxing for assistance with field collection. The authors also thank Zhang et al. for the primers used in this work.

Declaration of interest

The authors have no interests to declare. This work was supported by a startup grant from East China Normal University for X Mao.