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Abstract
We report complete chloroplast genome of the Tibetan hulless barely using IIlumina Hiseq 2000 sequencing technology (IIlumina Inc., San Diego, CA). The chloroplast genome size is 136 462 bp in length that includes two inverted repeats (IRs) of 10 528 bp, which are separated by the large single copy (LSC 101 375 bp) and small single copy (SSC 14 030 bp). Hulless barely chloroplast genome encodes 76 protein-coding genes, four rRNA genes, and 29 tRNA genes. The maximum-likelihood (ML) phylogenetic tree of the nine complete chloroplast genomes was selected from Poaceae family using Oryza sativa japonica as the out-group, supporting that hulless barely is closely related to the Hordeum vulgare subsp. vulgare.
Acknowledgements
The author would thank the editor and revivers for providing valuable comments on the manuscript.
Declaration of interest
The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper. This work was supported by the following funding sources: The National Science and Technology Support Program (2012BAD03B01 and 2013BAD30B02), and the Tibet Autonomous Region Financial Special Fund (2014CZZX001 and 2015ZC001).