Population genetic structure of Macrobrachium rosenbergii (Palaemonidae) from Indian waters using mitochondrial ATPase 6/8 gene

Abstract

Macrobrachium rosenbergii, giant freshwater prawn, is one of the most commercially important crustaceans. In the present study, primers for ATPase 6/8 region of mt-DNA were designed and successfully amplified (827 bp) in the species. The nucleotide variation in ATPase 6/8 gene revealed the population structuring in natural populations of M. rosenbergii in Indian waters. A total of 35 haplotypes were observed in 93 individuals collected from different locations. Low nucleotide diversity and high haplotype diversity were noticed for the ATPase 6/8 gene. Significant pairwise F_ST and, haplotype network indicated occurrence of distinct populations. Observed mismatch distribution and Tajima’s D test suggested demographical stability of giant freshwater prawn. The genetic stock structure revealed in this study will be helpful for conservation and management of stocks of M. rosenbergii in Indian waters.

Keywords:

• ATPase gene
• genetic stocks
• Macrobrachium rosenbergii
• population structure

Disclosure statement

The present study is a part of Outreach Activity on Fish Genetic Stocks and funded by Indian Council of Agriculture Research.