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Mitochondrial DNA Part A
DNA Mapping, Sequencing, and Analysis
Volume 28, 2017 - Issue 4
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Short Communication

DNA barcoding and phylogeny of Calidris and Tringa (Aves: Scolopacidae)

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Pages 616-619 | Received 09 Nov 2015, Accepted 13 Feb 2016, Published online: 17 Mar 2016
 

Abstract

The avian genera Calidris and Tringa are the largest of the widespread family of Scolopacidae. The phylogeny of members of the two genera is still a matter of controversial. Mitochondrial cytochrome c oxidase subunit I (COI) can serve as a fast and accurate marker for the identification and phylogeny of animal species. In this study, we analyzed the COI barcodes of thirty-one species of the two genera. All the species had distinct COI sequences. Two hundred and twenty-one variable sites were identified. Kimura two-parameter distances were calculated between barcodes. Neighbor-joining and maximum likelihood methods were used to construct phylogenetic trees. All the species could be discriminated by their distinct clades in the phylogenetic trees. The phylogenetic trees grouped all the species of Calidris and Tringa into different monophyletic clade, respectively. COI data showed a well-supported phylogeny for Calidris and Tringa species.

Disclosure statement

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the article.

Funding information

This work was supported by the National Natural Science Foundation of China (31260088, 31560590), Jiangxi Province Talent Project 555, Jiangxi Province Major Disciplines Academic Leaders (20133BCB22010), the Natural Science Foundation of Jiangxi Province (20132BAB204022, 20152ACB21006) and the Science and Technology Foundation of Jiangxi Provincial Department of Education (GJJ150768).

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