Morphological and molecular identification of four new resupinate species of Perenniporia (Polyporales) from southern China

Abstract

Four new resupinate species, Perenniporia hainaniana, P. macropora, P. russeimarginata and P. subtephropora, are described as new from southern China on the basis of morphological and molecular examination. Perenniporia hainaniana is characterized by a perennial growth habit, cream to buff pore surface, a dimitic to trimitic hyphal system with amyloid skeletal hyphae and presence of dendrohyphidia. Perenniporia macropora is distinguished by an annual growth habit, large pores (2–3 per mm), a dimitic hyphal system with branched skeletal hyphae and presence of dendrohyphidia. Perenniporia russeimarginata differs from other species in the genus by a perennial growth habit, white to cream pore surface and reddish to reddish brown sterile margin. Perenniporia subtephropora is characterized by a perennial growth habit, cream-buff to grayish buff pore surface, hymenophoral trama becoming black in KOH and a dimitic hyphal system with branched skeletal hyphae. Phylogenetic analysis inferred from the ITS and LSU-rDNA regions revealed eight clades for Perenniporia sensu lato with the four new species recognized in Perenniporia sensu stricto. The Perenniporia ochroleuca group, P. vicina group, P. martia group, P. subacida, Microporellus, Perenniporiella and Abundisporus, formed monophyletic entities respectively, and they are not related to P. medulla-panis (generic type), which belongs to Perenniporia sensu stricto. Abundisporus, Microporellus and Perenniporiella were widely applied as generic names. Other generic or clade names, Hornodermoporus, Truncospora, Vanderbylia and the P. subacida clade, have not been widely applied, but they are valid higher level taxa containing former species of Perenniporia. A key to genera and clades allied with Perennioporia sensu stricto and a key to species of Perennioporia are provided.

• phylogeny
• polypore
• taxonomy
• wood-inhabiting fungi

Acknowledgments

Special thanks to Drs Shuang-Hui He and Hai-Jiao Li (BJFC, China) and Hai-Sheng Yuan (IFP, China) for help in collecting specimens. We thank Dr Zheng Wang (Yale University, USA) and Prof Yu-Cheng Dai (IFP, China) for improving the manuscript. The research is financed by the Fundamental Research Funds for the Central Universities (Project No. BLYJ201205, JC2013-1), the National Natural Science Foundation of China (Project Nos. 30900006, 31093440) and the Program for New Century Excellent Talents in University (NCET-11-0585).