Review on In Vivo Profiling of Drug Metabolites with Lc-Ms/Ms in the Past Decade

Abstract

Metabolite profiling is an indispensable part of drug discovery and development, enabling a comprehensive understanding of the drug's metabolic behavior. Liquid chromatography-mass spectrometry facilitates metabolite profiling by reducing sample complexity and providing high sensitivity. This review discusses the in vivo metabolite profiling involving LC-MS/MS and the utilization of QTOF, QQQ mass analyzers with a particular emphasis on a mass filter. Further, a summary of sample extraction procedures in biological matrices such as plasma, urine, feces, serum and hair as in vivo samples are outlined. toward the end, we present 15 case studies in biological matrices and their LC-MS/MS conditions to understand the metabolic disposition.

Graphical abstract

Keywords::

• biotransformation
• LC-MS
• metabolite profiling
• plasma
• urine

Financial & competing interests disclosure

GN Reddy and C Laltanpuii are grateful to the Department of Pharmaceuticals, Ministry of Chemicals & Fertilizers, Government of India, New Delhi, for the NIPER fellowship award. The manuscript bears the NIPER-Hyderabad communication number NIPER-H/2021/176. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

No writing assistance was utilized in the production of this manuscript.