Abstract
A chromatographic immunoassay is a technique in which an antibody or antibody-related agent is used as part of a chromatographic system for the isolation or measurement of a specific target. Various binding agents, detection methods, supports and assay formats have been developed for this group of methods, and applications have been reported that range from drugs, hormones and herbicides to peptides, proteins and bacteria. This review discusses the general principles and applications of chromatographic immunoassays, with an emphasis being given to methods and formats that have been developed for the analysis of drugs and biological agents. The relative advantages or limitations of each format are discussed. Recent developments and research in this field, as well as possible future directions, are also considered.
Financial & competing interests disclosure
Some of the methods described in this review are the subjects of US Patents 6,720,193 and 6,727,104. Part of the work described in this review was supported by the NIH under grants R01 GM044931 and R01 DK069629 and by the National Science Foundation/EPSCoR program under grant EPS-1004094. D Suresh was supported by the University Grants Commission in India though a Raman Postdoctoral Fellowship. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.
No writing assistance was utilized in the production of this manuscript.