Abstract
Aim: The main objective of our studies was to determine the chemical composition and biological activities (antioxidant and anticoagulant properties) of two standardized phenolic fractions from sea buckthorn twig and leaf, and two standardized nonpolar fractions from twig and leaf in human plasma in vitro. Material & methods: Appropriately prepared extracts from sea buckthorn twigs and leaves were used. Markers of oxidative stress and hemostasis were determined in this work. Results: The reduction of plasma lipid peroxidation induced by H2O2/Fe was observed for two fractions from twig. Analysis of the effect on the coagulation properties of plasma demonstrated that the nonpolar fraction from twig and the phenolic fraction from leaf, significantly prolonged the activated partial thromboplastin time and the prothrombin time, respectively. Conclusion: Sea buckthorn twig and leaf are new promising plant materials in the prophylaxis and treatment of cardiovascular disorders.
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Acknowledgements
The authors would like to thank M Kowalczyk for performing the UHPLC–MS analyses and M Sobolewski (student, Faculty of Biology and Environmental Protection, University of Lodz) for skilled technical assistance.
Financial & competing interests disclosure
This work was supported by National Science Centre, Poland 2015/19/B/NZ9/03164. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.
No writing assistance was utilized in the production of this manuscript.