https://orcid.org/0000-0002-2817-7516
Abstract
Aim: To evaluate the effects of a novel glycogen phosphorylase inhibitor (NGPI) on cerebral ischemia–reperfusion injury (CIRI). Methods: Cerebral ischemia was induced in mice using a modified bilateral common carotid artery ligation model. To assess the effects of NGPI against CIRI, mice which had been administered with different doses of NGPI (1.25, 2.5, 5 mg/kg/day) for 7 days before the injury were evaluated for infarct volume, the apoptosis level of brain tissue, integrity of brain tissue and oxidative stress level. Results: NGPI effectively improved the infarct area, apoptosis of neurons, integrity of brain tissue and oxidative stress level of mice with CIRI. Conclusion: NGPI could effectively improve CIRI and deserves further study.
Author contributions
Conceived and designed the study: L Zhang and Z Yan. Performed the experiments: Z Yan and L Zhang. Analyzed the data: Z Yan and Y Wang. Contributed reagents/materials/analysis tools: L Zhang, Z Yan, Y Wang and Y Guo. Wrote the manuscript: L Zhang, Z Yan and S Li.
Financial & competing interests disclosure
The authors acknowledge the funds from the Natural Science Foundation of Hebei Province (no. H2021406034), the Key Subject Construction Project of Hebei Provincial College, Hebei (Chengde) Industrial Technology Institute of Chinese Medicinal Materials (in preparation) and Technology Innovation Guidance Project-Science and Technology Work Conference of Hebei Provincial Department of Science and Technology. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.
No writing assistance was utilized in the production of this manuscript.
Ethical conduct of research
The authors state that they have obtained appropriate institutional review board approval or have followed the principles outlined in the Declaration of Helsinki for all human or animal experimental investigations. The animals were housed and cared for in accordance with the guidelines established by the National Science Council of the Republic of China. All experimental protocols were approved by the Animal Care and Use Committee of Chengde Medical University, and all experiments were performed in accordance with the approved guidelines.