Alginate-combined Cholic Acid Increased Insulin Secretion of Microencapsulated Mouse Cloned Pancreatic β Cells

Abstract

Aim: A semisynthetic primary bile acid (PBA) has exerted hypoglycemic effects in Type 1 diabetic animals, which were hypothesized to be due to its anti-inflammatory and cellular glucose-regulatory effects. Thus, the research purpose aimed to examine antidiabetic effects of a PBA, in terms of cellular inflammation and survival and insulin release, in the context of supporting β-cell delivery and Type 1 diabetic treatment. Materials & methods: 10 formulations were prepared, five without PBA (control) and five with PBA (test). Formulations were used to microencapsulate pancreatic β cells and the microcapsules were examined for morphology, cell viability, insulin release and inflammation. Results & conclusion: PBA improved cell viability, insulin release and reduced inflammation in a formulation-dependent manner, which suggests potential use in cell delivery and diabetes treatment.

Keywords:

• artificial cell microencapsulation
• bile acid
• pancreatic β cells

Acknowledgements

The authors are thankful for the use of laboratory equipment, scientific and technical assistance of the Curtin University, microscopy and microanalysis facility, which has been partially funded by the University, State and Commonwealth Governments. The authors also thank the ARC Centre of Excellence in Plant Energy Biology at the University of Western Australia for training, support and access to equipment.

Financial & competing interests disclosure

The authors have received Australian Postgraduate Award and Curtin Research Scholarship. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

No writing assistance was utilized in the production of this manuscript.