Phosphatase inhibition and cell survival after DNA damage induced by radiation

Abstract

It has come to our attention that there are errors in the figure legends to our manuscript. In Figure 5, Panel I was not labeled thus the description of this panel in the legend to Figure 5 should read, “Western blot analysis of AKT (H) shows no effect on the phosphorylation of S473 or total protein levels with either T24 cells (Left) or SQ20B cell (Right).”

In the legend to Figure 6, PHLPP and PHLPPL were inadvertently reversed. Also the description of panel D was omitted. The figure legend should read. “Figure 6. Analysis of PHLPP and PHLPPL expression following siRNA treatment. (A) Western blot analysis was used to determine the knockdown of PHLPP and PHLPPL as well as the phosphorylation status of AKT. 20 μg of total protein was loaded for each western blot and β-actin used as a loading control. Lanes 1 are non-targeting siRNA treated lysates and lanes 2 are specific siRNA treated lysates.  SQ20B, T24, DLD-1 and HT1080 cells were transfected with 40 nM non-targeting siRNA or siRNA against (B) PHLPPL and (C) PHLPP or with a combination of both PHLPPL and PHLPP siRNA (D) for 48 hr. Cells were replated and allowed to adhere before irradiated at 0, 2, 4 and 6 Gy and the medium changed the nextday. Clonogens were allowed to form for 10–14 days before staining with crystal violet. Solid lines indicate non-targeting siRNA and dashed lines indicate targeted siRNA.”

We sincerely regret these errors and any confusion they may cause.  The corrections do not in any way alter the conclusions of the manuscript.

This article refers to:

Phosphatase inhibition and cell survival after DNA damage induced by radiation