Mechanically induced deformation and strain dynamics in actin stress fibers

Abstract

It is becoming evident that physical forces in the microenvironment play a key role in regulating many important aspects of cell biology. However, although mechanical cues are known to have clear effects over the long-term (days), the short-term (seconds to minutes) cellular responses to mechanical stimuli are less well characterized. In our recent study, we exposed committed fibroblast cells to well controlled nanoscale forces while simultaneously imaging force transduction through the actin cytoskeleton. One of the earliest responses of a cell to physical force is rapid deformation of the cytoskeleton, taking place over the course of seconds. We were able to directly visualize deformation, force-propagation and strain dynamics in actin stress fibers in response to a relatively simple mechanical stimulus. Moreover, these dynamics were also dependent on myosin-driven contractility and the presence of an intact microtubule cytoskeleton. Interestingly, although stem cells are sensitive to mechanical cues, they do not display the same degree of stress fiber organization as observed in committed cells indicating the possibility of alternative sensing and mechanotransduction mechanisms.

Keywords: :

• actin
• strain
• mechanotransduction
• nanomechanics
• cytoskeleton

The cellular cytoskeleton is a complex interconnected network, composed of a dynamic and complex array of structural and regulatory proteins that permit the cell to adapt the many cues in its microenvironment.¹ Specifically, living cells actively respond to mechanical forces and changes in the material properties of the microenvironment.^2,3 These mechanical cues have a significant role to play in regulating and controlling cell biology and behavior.^2,3 The process of mechanotransduction (the conversion of mechanical cues into biological outcomes) is under intense investigation as these pathways play key roles in a diverse number of processes including, determining stem cell fate, malignancy, migration, proliferation and myogenesis.^4-8 Importantly, these processes take place over days to weeks and indicate the importance of mechanical cues acting over long timescales.

Of particular importance in the ability of a cell to respond to its mechanical microenvironment are actin stress fibers. These structures are constantly undergoing a state of reorganization, transmitting and relaying nanomechanical forces in the process. The cytoskeleton acts as an interface, intricately connecting the cell to the external microenvironment through physical connections at focal adhesion and integrin sites.^1,3 Indeed, acto-myosin contractility plays a large role in the ability of cells to sense the mechanical properties of their microenvironment through the generation of traction forces.⁹ Importantly, the physical properties of the microenvironment are also well known to alter cytoskeletal organization and cell behavior demonstrating the intimate link between the cytoarchitecture and the microenvironment.^1,3 In addition to this, local forces applied to the cell membrane can result in force transduction through the cell, activation of mechanosensitive ion channels, secondary messenger release and gene regulation.^10-17 As opposed to the processes discussed above, these events are activated rapidly, taking place on the second to minute timescale. However, the short-term response of a cell to mechanical force remains poorly understood even though it has a major role to play in the long-term outcome.¹¹

We recently generated NIH3T3 fibroblast cells transiently expressing actin-EGFP and mechanically stimulated them with well-controlled nanonewton forces with an atomic force microscope (AFM) mounted on a laser scanning confocal microscope (LSCM) (Fig. 1A).¹¹ This approach allowed us to simultaneously expose cells to local nanonewton forces while quantifying force transduction through the actin cytoskeleton. With the AFM tip directly over the nucleus, forces from 0nN to 20nN were applied and actin deformation and force transduction was quantified. These studies revealed that following a locally applied force, the F-actin cytoskeleton does not undergo a global isotropic deformation, but rather, highly localized and heterogeneous deformation occurring throughout the cell (Fig. 1B). Two distinct actin displacements were observed: an initial localized deformation occurring within ~20μm from the point of contact (but not at the point of contact), followed by a localized deformation far from the point of contact, including the retraction of distant stress fibers at the cell edges due to focal adhesion remodeling. To assess the involvement of the microtubule network, cells were pre-treated with 10μM of nocodazole, a microtubule polymerizing inhibitor.¹¹ Results indicate that the actin cytoskeleton underwent a much smaller deformation, comparable to that of control cells not subjected to any force. This suggests that the microtubule network is highly involved in anisotropic force transduction pathways observed following a nanomechanical force. In addition, we utilized an approach in which actin stress fibers were patterned into segments by photobleaching the EGFP every 5μm along their length (Fig. 2A).^11,18,19 This allowed us to quantify any local contraction or stretching (strain) of stress fibers by simply quantifying changes in length of each individual segment. We observed that stress fibers underwent heterogeneous strain fluctuations all along their length, exhibiting a complex array of regions undergoing stretch and contraction (Fig. 2B). In response to mechanical stimulation we observed a ~50% increase in the magnitude of the strain fluctuations along stress fibers and no clear signs of concerted contraction or stretch along the entire length of the fiber. These results imply that the elasticity (or Young’s modulus) of actin stress-fibers varies along their length. This is likely due to the cross-linked nature of the actin cytoskeleton. Regions more highly cross-linked will have a high effective elasticity than regions with fewer crosslinks. Therefore, acto-myosin driven contractility would cause strain dynamics that would appear heterogeneous.

Figure 1. Applying local mechanical forces to living NIH3T3 cells with simultaneous AFM and LSCM. (A) An NIH3T3 cells transiently expressing actin-EGFP. The AFM cantilever is positioned above the cell nucleus and used to apply local nanonewton forces. (B) Heat map lateral actin deformation in a cell exposed to a 20nN stimulus at the position marked with an ‘x’. Highly localized deformations take place far form the point of contact and evolve in time. Experimental details in reference 11. Figure adapted from reference 11. Scale bars in both (A) and (B) are 15 μm.

Figure 2. Quantifying strain dynamics in actin stress fibers. (A) Actin-EGFP stress fibers photobleached every 5 μm to create a segmented appearance (stress fibers do remain intact). As the stress fibers undergo stretch and contraction the segments will change length allowing the quantification of internal strain. (B) Strain dynamics taking place along stress fibers are heterogeneous and evolve in time. Negative strain values correspond to regions undergoing contraction and positive strain values correspond to regions undergoing stretch. Experimental details in reference 11. Figure adapted from reference 11. Scale bars in both (A) and (B) are 15 μm.

Our study has demonstrated that force-induced cytoskeletal remodeling occurs through a complex and localized response that is highly dependent on the presence of myosin-driven contractility and an intact microtubule network. A possible explanation¹¹ for the observed strain dynamics involves increased Ca²⁺ concentration following an applied force, which would induce a calmodulin-dependent activation of myosin light chain kinase (MLCK). Myosin II is phosphorylated by this kinase in addition to ROCK (rho kinase), causing an increase in stress fiber formation and contraction.

Interestingly, uncommitted stem cells are well known to exhibit extreme sensitivity to mechanical cues occurring in the microenvironment. Stem cells will commit to a particular lineage based on the elasticity of the matrix on which they are propagating. As well, local and global mechanical stresses occurring in the microenvironment can also stimulate cells to commit to a particular fate.^20,21 However, as demonstrated in another previous study from our lab,²² mouse embryonic stem cells (E14 and ES-D3 cell lines) do not display well-defined actin stress-fibers comparable to those observed in committed cells (Fig. 3). Conversely, we found that these cells display an intact microtubule cytoskeleton. Moreover, it has been well documented that stem cells generally display a poorly organized and diffuse actin cytoskeleton in soft microenvironments.^7,23,24 Therefore, we speculate that uncommitted stem cells may possess alternative, yet complementary, pathways to sense and respond to mechanical cues occurring in their microenvironment. Importantly, it is becoming increasingly recognized that microtubules also play an important role in governing the mechanical properties of the cell as they play a role in generating traction forces and mediating focal adhesion dynamics.^25-27 Our recent study¹¹ has revealed that local nanoscale forces are rapidly and anisotropically transduced and focused throughout the cell body. However, these pathways are unlikely to be universal, rather they are likely dependent on cell type, the mechanical properties of the microenvironment and possibly even the development stage of the cell. This rich biological complexity provides many opportunities for the study of the fundamental mechanical pathways that have such clear importance in diverse aspects of cell biology.

Figure 3. Actin stress fibers in (A) NIH3T3 and (B) pluripotent ES-D3 mouse embryonic stem cells (mESCs). Cells were fixed and stained for actin (phalloidin conjugated to Alexa Fluor 546, white) and the nucleus (DAPI, blue). Stress fibers are numerous and highly organized in committed NIH3T3 cells. Pluripotent mESCs (confirmed by staining for the presence of the transcription factor OCT4, not shown) are generally significantly smaller than NIH3T3 cells and display fewer stress fibers. The actin is generally diffuse and poorly organized. However, small punctate actin protrusions are observed. Experimental details in reference 11. Scale bars in both (A) and (B) are 20 μm.

Disclosure of Potential Conflict of Interest

No potential conflicts of interest were disclosed.

Acknowledgments

L.G. was supported by graduate fellowships from the Natural Sciences and Engineering Research Council (NSERC) and the Ontario Graduate Scholarships program. A.E.P. gratefully acknowledges generous support from the Canada Research Chairs (CRC) program and a Province of Ontario Early Researcher Award. This work was supported by an NSERC Discovery Grant, an NSERC Discovery Accelerator Supplement and a CRC Operating Grant.