- Hooper DC.Mechanisms of action of antimicrobials: focus on fluoroquinolones. Clin Infect Dis2001;32 (Suppl 1): S9–S15.
- Dalhoff A.Global Fluoroquinolone resistance epidemiology and implictions for clinical use. Interdiscip Perspect Infect Dis2012;2012: 976273.
- Khawcharoenporn T, Vasoo S, Ward E, Singh K.High rates of quinolone resistance among urinary tract infections in the ED. Am J Emerg Med2012;30: 68–74.
- Morgan-Linnell SK, Becnel Boyd L, Steffen D et al.Mechanisms accounting for fluoroquinolone resistance in Escherichia coli clinical isolates. Antimicrob Agents Chemother2009;53 235–241.
- Jacoby GA.Mechanisms of resistance to quinolones. Clin Infect Dis2005;41 (Suppl 2): S120–S126.
- Komp Lindgren P, Karlsson A, Hughes D.Mutation rate and evolution of fluoroquinolone resistance in Escherichia coli isolates from patients with urinary tract infections. Antimicrob Agents Chemother2003;47: 3222–3232.
- Wang H, Dzink-Fox JL, Chen M et al.Genetic characterization of highly fluoroquinolone-resistant clinical Escherichia coli strains from China: role of acrR mutations. Antimicrob Agents Chemother2001;45: 1515–1521.
- Maneewannakul K, Levy SB.Identification for mar mutants among quinolone-resistant clinical isolates of Escherichia coli. Antimicrob Agents Chemother1996;40: 1695–1698.
- Koutsolioutsou A, Pena-Llopis S, Demple B.Constitutive soxR mutations contribute to multiple-antibiotic resistance in clinical Escherichia coli isolates. Antimicrob Agents Chemother2005;49: 2746–2752.
- Barnard FM, Maxwell A.Interaction between DNA gyrase and quinolones: effects of alanine mutations at GyrA subunit residues Ser83 and Asp87. Antimicrob Agents Chemother2001;45: 1994–2000.
- Heisig P.Genetic evidence for a role of parC mutations in development of high-level fluoroquinolone resistance in Escherichia coli. Antimicrob Agents Chemother1996;40: 879–885.
- Koutsolioutsou A, Martins EA, White DG et al.A soxRS-constitutive mutation contributing to antibiotic resistance in a clinical isolate of Salmonella enterica (Serovar Typhimurium). Antimicrob Agents Chemother2001;45: 38–43.
- Schneiders T, Amyes SGB, Levy SB.Role of AcrR and RamA in fluoroquinolone resistance in clinical Klebsiella pneumoniae isolates from Singapore. Antimicrob Agents Chemother2003;47: 2831–2837.
- Cavaco LM, Frimodt-Møller N, Hasman H et al.Prevalence of quinolone resistance mechanisms and associations to minimum inhibitory concentrations in quinolone-resistant Escherichia coli isolated from humans and swine in Denmark. Microb Drug Resist2008;14: 163–169.
- Clinical and Laboratory Standards Institute.Performance standards for antimicrobial susceptibility testing, 22nd informational supplement. CLSI document M100-S22.Wayne, PA: CLSI, 2012.Available at http://www.indabook.org/preview/8gaxEopPrDb_fkWdELy71yeEBjeFu0sVzLyJn7c_-eM,/M100-S22-Performance-Standards-for-Antimicrobial.html?query=M100-S22-Performance-Standards-for-Antimicrobial
- Kronvall G.Normalized resistance interpretation as a tool for establishing epidemiological MIC susceptibility breakpoints. J Clin Microbiol2010;48: 4445–4452.
- Aly MEA, Essam TM, Amin MA.Antibiotic resistance profile of E. coli strains isolated from clinical specimens and food samples in Egypt. Int J Microbiol Res2012;3: 176–182.
- Aly MEA, Essam TM, Amin MA.Involvement of virulence genes and antibiotic resistance in clinical and food borne diarrheagenic Escherichia coli isolates from Egypt. World J Med Sci2012;7: 276–284.
- Ye J, Coulouris G, Zaretskaya I et al.Primer-BLAST: a tool to design target-specific primers for polymerase chain reaction. BMC Bioinformatics2012;13: 134.
- Ma H, Difazio S.An efficient method for purification of PCR products for sequencing. BioTechniques2008;44: 921–923.
- Yamaguchi Y, Nimbari S, Ookawara T et al.Inhibitory effects of agarose gel and LB medium on DNA sequencing. BioTechniques2002;33: 282, 284.
- Sanger F, Nicklen S, Coulson AR.DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA1977;74: 5463–5467.
- Sievers F, Wilm A, Dineen D et al.Fast, scalable generation of high-quality protein multiple sequence alignments using Clustal Omega. Mol Syst Biol2011;7: 539.
- Arsene S, Leclercq R.Role of a qnr-like gene in the intrinsic resistance of Enterococcus faecalis to floroquinolones. Antimicrob Agents Chemother2007;51: 3254–3258.
- Singh R, Ledesma KR, Chang KT et al.Impact of recA on levofloxacin exposure-related resistance development. Antimicrob Agents Chemother2010;54: 4262–4268.
- Fu Y, Zhang W, Wang H et al.Specific patterns of gyrA mutations determine the resistance difference to ciprofloxacin and levofloxacin in Klebsiella pneumoniae and Escherichia coli. BMC Infect Dis2013;13: 8.
- Li J, Gao X, Luo T et al.Association of gyrA/B mutations and resistance levels to fluoroquinolones in clinical isolates of Mycobacterium tuberculosis. Emerg Microbes Infect2014;3: e19.
- Oethinger M, Kern WV, Jellen-Ritter AS et al.Ineffectiveness of topoisomerase mutations in mediating clinically significant fluoroquinolone resistance in Escherichia coli in the absence of the AcrAB efflux pump. Antimicrob Agents Chemother2000;44: 10–13.
- Webber MA, Talukder A, Piddock LJV.Contribution of mutation at amino acid 45 of AcrR to acrB expression and ciprofloxacin resistance in clinical and veterinary Escherichia coli isolates. Antimicrob Agents Chemother2005;49: 4390–4392.
- Swick MC, Evangelista MA, Bodine TJ et al.Novel conserved genotypes correspond to antibiotic resistance phenotypes of E. coli clinical isolates. PLoS ONE2013;8: e65961.
- Sulavik MC, Houseweart C, Cramer C et al.Antibiotic susceptibility profiles of Escherichia coli strains lacking multidrug efflux pump genes. Antimicrob Agents Chemother2001;45: 1126–1136.
- Shigemura K, Tanaka K, Yamamichi F et al.Does mutation in gyrA and/or parC or efflux pump expression play the main role in fluoroquinolone resistance in Escherichia coli urinary tract infections?: a statistical analysis study. Int J Antimicrob Agents2012;40: 516–520.
- Marcusson LL, Frimodt-Møller N, Hughes D.Interplay in the selection of fluoroquinolone resistance and bacterial fitness. PLoS Pathog2009;5: e1000541.
- Paltansing S, Kraakman MEM, Ras JMC et al.Characterization of fluoroquinolone and cephalosporin resistance mechanisms in Enterobacteriaceae isolated in a Dutch teaching hospital reveals the presence of an Escherichia coli ST131 clone with a specific mutation in parE. J Antimicrob Chemother2013;68: 40–45.
- Ciesielczuk H, Hornsey M, Choi Vet al.Development and evaluation of a multiplex PCR for eight plasmid-mediated quinolone-resistance determinants. J Med Microbiol2013;62(Pt 12): 1823–1827.
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Original Articles
‘Supermutators’ found amongst highly levofloxacin-resistant E. coli isolates: a rapid protocol for the detection of mutation sites
Ahmed Abdel-Fattah ZayedDepartment of Microbiology & Immunology, Faculty of Pharmacy, Ahram Canadian University, 6th of October City 12566, Egypt
, Tamer Mohamed EssamDepartment of Microbiology and Immunology and Biotechnology Centre, Faculty of Pharmacy, Cairo University, Cairo11562, EgyptCorrespondence[email protected]
, Abdel-Gawad Mohamed HashemDepartment of Microbiology and Immunology and Biotechnology Centre, Faculty of Pharmacy, Cairo University, Cairo11562, Egypt
& Ossama Mohamed El-TayebDepartment of Microbiology and Immunology and Biotechnology Centre, Faculty of Pharmacy, Cairo University, Cairo11562, Egypt
Pages 1-8
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Received 28 Sep 2014, Accepted 12 Dec 2014, Published online: 25 Jan 2019
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