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Original Articles

Expression and characterization of a Talaromyces marneffei active phospholipase B expressed in a Pichia pastoris expression system

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Pages 1-6 | Received 07 Jul 2016, Accepted 06 Sep 2016, Published online: 25 Jan 2019

  • HoubrakenJ,de VriesRP,SamsonRA.Modern taxonomy of biotechnologically important Aspergillus and Penicillium species.Adv Appl Microbiol2014; 86:199–249.
  • TamEW,TsangCC,LauSKet al.Polyketides, toxins and pigments in Penicillium marneffei.Toxins (Basel)2015; 7:4421–4436.
  • ChanJF,LauSK,YuenKYet al.Talaromyces (Penicillium) marneffei infection in non-HIV-infected patients.Emerg Microbes Infect2016; 5:e19.
  • HuY,ZhangJ,LiXet al.Penicillium marneffei infection: an emerging disease in mainland China.Mycopathologia2013; 175:57–67.
  • CánovasD,AndrianopoulosA.The biology of the thermally dimorphic fungal pathogen Penicillium marneffei. In:KavanaghK (ed). New Insights in Medical Mycology.Dordrecht, the Netherlands: Springer.2007,213–226.
  • LauSK,TseH,ChanJSet al.Proteome profiling of the dimorphic fungus Penicillium marneffei extracellular proteins and identification of glyceraldehyde-3-phosphate dehydrogenase as an important adhesion factor for conidial attachment.FEBS J2013; 280:6613–6626.
  • YangE,ChowWN,WangGet al.Signature gene expression reveals novel clues to the molecular mechanisms of dimorphic transition in Penicillium marneffei.PLoS Genet2014; 10:e1004662.
  • SapmakA,BoyceKJ,AndrianopoulosAet al.The pbrB gene encodes a laccase required for DHN-melanin synthesis in conidia of Talaromyces (Penicillium) marneffei.PLoS One2015; 10:e122728.
  • GhannoumMA.Potential role of phospholipases in virulence and fungal pathogenesis.Clin Microbiol Rev2000; 13:122–143.
  • ShenDK,NoodehAD,KazemiAet al.Characterisation and expression of phospholipases B from the opportunistic fungus Aspergillus fumigatus.FEMS Microbiol Lett2004; 239:87–93.
  • KöhlerGA,BrenotA,Haas-StapletonEet al.Phospholipase A2 and phospholipase B activities in fungi.Biochim Biophys Acta2006; 1761:1391–1399.
  • Karkowska-KuletaJ,Rapala-KozikM,KozikA.Fungi pathogenic to humans: molecular bases of virulence of Candida albicans, Cryptococcus neoformans and Aspergillus fumigatus.Acta Biochim Pol2009; 56:211–224.
  • MaL,XieLX,DongXGet al.[Virulence of extracellular phospholipase B of Candida albicans in rabbit experimental keratomycosis].Zhonghua Yan Ke Za Zhi2008; 44:237–243Chinese.
  • MaruvadaR,ZhuL,PearceDet al.Cryptococcus neoformans phospholipase B1 activates host cell Rac1 for traversal across the blood-brain barrier.Cell Microbiol2012; 14:1544–1553.
  • Macauley-PatrickS,FazendaML,McNeilBet al.Heterologous protein production using the Pichia pastoris expression system.Yeast2005; 22:249–270.
  • LiuH,XiL,ZhangJet al.Identifying differentially expressed genes in the dimorphic fungus Penicillium marneffei by suppression subtractive hybridization.FEMS Microbiol Lett2007; 270:97–103.
  • TamuraK,StecherG,PetersonDet al.MEGA6: Molecular Evolutionary Genetics Analysis version 6.0.Mol Biol Evol2013; 30:2725–2729.
  • DankaiW,PongpomM,VanittanakomN.Validation of reference genes for real-time quantitative RT-PCR studies in Talaromyces marneffei.J Microbiol Meth2015; 118:42–50.
  • LivakKJ,SchmittgenTD.Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method.Methods2001; 25:402–408.
  • SatouM,NishiY,YohJet al.Identification and characterization of acyl-protein thioesterase 1/lysophospholipase I as a ghrelin deacylation/lysophospholipid hydrolyzing enzyme in fetal bovine serum and conditioned medium.Endocrinology2010; 151:4765–4775.
  • KunzeD,MelzerI,BennettDet al.Functional analysis of the phospholipase C gene CaPLC1 and two unusual phospholipase C genes, CaPLC2 and CaPLC3, of Candida albicans.Microbiology2005; 151(Pt 10):3381–3394.
  • MendonsaR,EngebrechtJ.Phospholipase D function in Saccharomyces cerevisiae.Biochim Biophys Acta2009; 1791:970–974.
  • LiX,GaoM,HanXet al.Disruption of the phospholipase D gene attenuates the virulence of Aspergillus fumigatus.Infect Immun2012; 80:429–440.
  • YangE,WangG,WooPCet al.Unraveling the molecular basis of temperature-dependent genetic regulation in Penicillium marneffei.Eukaryot Cell2013; 12:1214–1224.
  • WongH,SchotzMC.The lipase gene family.J Lipid Res2002; 43:993–999.
  • PortillaD,CrewMD,GrantDet al.cDNA cloning and expression of a novel family of enzymes with calcium-independent phospholipase A2 and lysophospholipase activities.J Am Soc Nephrol1998; 9:1178–1186.
  • WooPC,ZhenH,CaiJJet al.The mitochondrial genome of the thermal dimorphic fungus Penicillium marneffei is more closely related to those of molds than yeasts.FEBS Lett2003; 555:469–477.
  • LiuR,ZhaoB,ZhangYet al.High-level expression, purification, and enzymatic characterization of truncated human plasminogen (Lys531-Asn791) in the methylotrophic yeast Pichia pastoris.BMC Biotechnol2015; 15:50.
  • SpohnerSC,MullerH,QuitmannHet al.Expression of enzymes for the usage in food and feed industry with Pichia pastoris.J Biotechnol2015; 202:118–134.