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Emerging Microbes & Infections Volume 5, 2016 - Issue 1
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Original Articles

Predominance and high antibiotic resistance of the emerging Clostridium difficile genotypes NAPCR1 and NAP9 in a Costa Rican hospital over a 2-year period without outbreaks

Diana López-UreñaLaboratory of Research in Anaerobic Bacteriology and Research Center in Tropical Diseases, University of Costa Rica, San José11501-2060, Costa Rica
,
Carlos Quesada-GómezLaboratory of Research in Anaerobic Bacteriology and Research Center in Tropical Diseases, University of Costa Rica, San José11501-2060, Costa Rica
,
Mónica Montoya-RamírezLaboratory of Research in Anaerobic Bacteriology and Research Center in Tropical Diseases, University of Costa Rica, San José11501-2060, Costa Rica
,
María del Mar Gamboa-CoronadoLaboratory of Research in Anaerobic Bacteriology and Research Center in Tropical Diseases, University of Costa Rica, San José11501-2060, Costa Rica
,
Teresita SomogyiLaboratory of Research in Anaerobic Bacteriology and Research Center in Tropical Diseases, University of Costa Rica, San José11501-2060, Costa Rica
,
César RodríguezLaboratory of Research in Anaerobic Bacteriology and Research Center in Tropical Diseases, University of Costa Rica, San José11501-2060, Costa Rica
&
Evelyn Rodríguez-CavalliniLaboratory of Research in Anaerobic Bacteriology and Research Center in Tropical Diseases, University of Costa Rica, San José11501-2060, Costa RicaCorrespondence[email protected]
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Pages 1-5 | Received 08 Oct 2015, Accepted 07 Jan 2016, Published online: 25 Jan 2019

  • SlimingsC,RileyT.Antibiotics and hospital-acquired Clostridium difficile infection: Update of systematic review and meta-analysis.J Antimicrob Chemother2014; 69:881–891.
  • ShivashankarR,KhannaS,KammerPPet al.Clinical factors associated with development of severe-complicated Clostridium difficile infection.Clin Gastroenterol Hepatol2013; 11:1466–1471.
  • AktoriesK.Bacterial protein toxins that modify host regulatory GTPases.Nat Rev Microbiol2011; 9:487–498.
  • KimH,KokkotouE,NaXet al.Clostridium difficile toxin A-induced colonocyte apoptosis involves p53-dependent p21(WAF1/CIP1) induction via p38 mitogen-activated protein kinase.Gastroenterology2005; 129:1875–1888.
  • ChumblerNM,FarrowMA,LapierreLAet al.Clostridium difficile toxin B causes epithelial cell necrosis through an autoprocessing-independent mechanism.PLoS Pathog2012; 8:e1003072.
  • DingleKE,ElliottB,RobinsonEet al.Evolutionary history of the Clostridium difficile pathogenicity locus.Genome Biol Evol2014; 6:36–52.
  • DupuyB,GovindR,AntunesAet al.Clostridium difficile toxin synthesis is negatively regulated by TcdC.J Med Microbiol2008; 57:685–689.
  • CartmanST,KellyML,HeegDet al.Precise manipulation of the Clostridium difficile chromosome reveals a lack of association between the tcdc genotype and toxin production.Appl Environ Microbiol2012; 78:4683–4690.
  • LanisJM,HeinlenLD,JamesJAet al.Clostridium difficile 027/BI/NAP1 encodes a hypertoxic and antigenically variable form of TcdB.PLoS Pathog2013; 9:e1003523.
  • GerdingDN,JohnsonS,RupnikMet al.Clostridium difficile binary toxin CDT: mechanism, epidemiology, and potential clinical importance.Gut Microbes2014; 5:15–27.
  • SchwanC,StecherB,TzivelekidisTet al.Clostridium difficile toxin CDT induces formation of microtubule-based protrusions and increases adherence of bacteria.PLoS Pathog2009; 5:e1000626.
  • KuehneSA,ColleryMM,KellyMLet al.Importance of toxin A, toxin B, and CDT in virulence of an epidemic Clostridium difficile strain.J Infect Dis2014; 209:83–86.
  • ElliottB,SquireMM,TheanSet al.New types of toxin A-negative, toxin B-positive strains among clinical isolates of Clostridium difficile in Australia.J Med Microbiol2011; 60:1108–1111.
  • MerriganM,VenugopalA,MallozziMet al.Human hypervirulent Clostridium difficile strains exhibit increased sporulation as well as robust toxin production.J Bacteriol2010; 192:4904–4911.
  • KimJ,PaiH,SeoM-Ret al.Clinical and microbiologic characteristics of tcdA-negative variant Clostridium difficile infections.BMC Infect Dis2012; 12:109.
  • RazaviB,ApisarnthanarakA,MundyLM.Clostridium difficile: emergence of hypervirulence and fluoroquinolone resistance.Infection2007; 35:300–307.
  • Chaves-OlarteE,FreerE,ParraAet al.R-Ras glucosylation and transient RhoA activation determine the cytopathic effect produced by toxin B variants from toxin A-negative strains of Clostridium difficile.J Biol Chem2003; 278:7956–7963.
  • Quesada-GómezC,RodríguezC,Gamboa-CoronadoMDMet al.Emergence of Clostridium difficile NAP1 in Latin America.J Clin Microbiol2010; 48:669–670.
  • Quesada-GómezC,López-UreñaD,Acuña-AmadorLet al.Emergence of an outbreak-associated Clostridium difficile variant with increased virulence.J Clin Microbiol2015; 53:1216–1226.
  • CohenSH,GerdingDN,JohnsonSet al.Clinical practice guidelines for Clostridium difficile infection in adults: 2010 update by the society for healthcare epidemiology of America (SHEA) and the infectious diseases society of America (IDSA).Infect Control Hosp Epidemiol2010; 31:431–455.
  • SpigagliaP,MastrantonioP.Comparative analysis of Clostridium difficile clinical isolates belonging to different genetic lineages and time periods.J Med Microbiol2004; 53:1129–1136.
  • Letournel-GlomaudC,HoussayeS,MilhaihaLet al.E-test antibiotics susceptibility of strict anaerobic bacteria.Anaerobe2003; 9:281–284.
  • Clinical and Laboratory Standards InstituteMethods for Antimicrobial Susceptibility Testing of Anaerobic Bacteria,Approved Standard, CLSI document M11-A8,CLSI: Wayne, PA, USA.2012.Available athttp://shop.clsi.org/site/Sample_pdf/M11A8_sample.pdf.
  • DrudyD,HarnedyN,FanningSet al.Emergence and control of fluoroquinolone-resistant, toxin A–negative, toxin B–positive Clostridium difficile.Infect Control2007; 28:932–940.
  • DuP,CaoB,WangJet al.Sequence variation in tcdA and tcdB of Clostridium difficile: ST37 with truncated tcdA is a potential epidemic strain in China.J Clin Microbiol2014; 52:3264–3270.
  • CollinsD,HawkeyP,RileyT.Epidemiology of Clostridium difficile infection in Asia.Antimicrob Resist Infect Control2013; 2:21.
  • GoorhuisA,LegariaMC,van den BergRJet al.Application of multiple-locus variable-number tandem-repeat analysis to determine clonal spread of toxin A-negative Clostridium difficile in a general hospital in Buenos Aires, Argentina.Clin Microbiol Infect2009; 15:1080–1086.
  • AlfaMJ,KabaniA,LyerlyDet al.Characterization of a toxin A-negative, toxin B-positive strain of Clostridium difficile responsible for a nosocomial outbreak of Clostridium difficile-associated diarrhea.J Clin Microbiol2000; 38:2706–2714.
  • ThakurS,SandfossM,Kennedy-StoskopfSet al.Detection of Clostridium difficile and Salmonella in feral swine population in North Carolina.J Wildl Dis2011; 47:774–776.
  • JohnsonS,SambolSP,BrazierJSet al.International typing study of toxin A-negative, toxin B-positive Clostridium difficile variants.J Clin Microbiol2003; 41:1543–1547.
  • Van den BergRJ,ClaasECJ,OyibDHet al.Characterization of toxin A-negative, toxin B-positive Clostridium difficile isolates from outbreaks in different countries by amplified fragment length polymorphism and PCR ribotyping.J Clin Microbiol2004; 42:1035–1041.
  • GriffithsD,FawleyW,KachrimanidouMet al.Multilocus sequence typing of Clostridium difficile.J Clin Microbiol2010; 48:770–778.
  • MartinH,WilleyB,LowDEet al.Characterization of Clostridium difficile strains isolated from patients in Ontario, Canada, from 2004 to 2006.J Clin Microbiol2008; 46:2999–3004.
  • LessaFC.Community-associated Clostridium difficile infection: how real is it?Anaerobe2013; 24:121–123.
  • MolinaJ,BarrantesG,Quesada-GómezCet al.Phenotypic and genotypic characterization of multidrug-resistant BacteroidesParabacteroides spp., and Pseudoflavonifractor from a Costa Rican hospital.Microb Drug Resist2014; 20:478–484.
  • Quesada-GómezC,Rodríguez-CavalliniE,RodríguezC.Scarce detection of mobile erm genes associated with tetQ in Bacteroides and Parabacteroides from Costa Rica.Anaerobe2013; 21:18–21.
  • RashidM-U,WeintraubA,NordCE.Development of antimicrobial resistance in the normal anaerobic microbiota during one year after administration of clindamycin or ciprofloxacin.Anaerobe2015; 31:72–77.
  • HuangH,WeintraubA,FangHet al.Antimicrobial resistance in Clostridium difficile.Int J Antimicrob Agents2009; 34:516–522.
  • TicklerIA,GoeringRV,WhitmoreJDet al.Strain types and antimicrobial resistance patterns of Clostridium difficile isolates from the United States, 2011 to 2013.Antimicrob Agents Chemother2014; 58:4214–4218.
  • SpigagliaP,BarbantiF,LouieTet al.Molecular analysis of the gyrA and gyrB quinolone resistance-determining regions of fluoroquinolone-resistant Clostridium difficile mutants selected in vitro.Antimicrob Agents Chemother2009; 53:2463–2468.
  • BarbutF,MastrantonioP,DelméeMet al.Prospective study of Clostridium difficile infections in Europe with phenotypic and genotypic characterisation of the isolates.Clin Microbiol Infect2007; 13:1048–1057.
  • BourgaultA-M,LamotheF,LooVGet al.In vitro susceptibility of Clostridium difficile clinical isolates from a multi-institutional outbreak in Southern Québec, Canada.Antimicrob Agents Chemother2006; 50:3473–3475.
  • HuangH,WuS,WangMet al.Clostridium difficile infections in a Shanghai hospital: antimicrobial resistance, toxin profiles and ribotypes.Int J Antimicrob Agents2009; 33:339–342.
  • CurrySR,MarshJW,ShuttKAet al.High frequency of rifampin resistance identified in an epidemic Clostridium difficile clone from a large teaching hospital.Clin Infect Dis2009; 48:425–429.
  • SpigagliaP,BarbantiF,MastrantonioP.Multidrug resistance in European Clostridium difficile clinical isolates.J Antimicrob Chemother2011; 66:2227–2234.
  • O’ConnorJR,GalangMA,SambolSPet al.Rifampin and rifaximin resistance in clinical isolates of Clostridium difficile.Antimicrob Agents Chemother2008; 52:2813–2817.

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