Alkaline protease-deficient mutants of Pseudomonas aeruginosa are virulent in the eye

Reference

• Laibson PR. Annual review: cornea and sclera. Arch Opthalmol. 1972;88:630–648.
   Google Scholar
• Burd EM. Bacterial keratitis and conjunctivitis. In: Smolin G, Thoft RA (eds.) The cornea. Boston, MA, USA; Little, Brown and Co.; 1994:115–124.
   Google Scholar
• Limberg MB. A review of bacterial keratitis and bacterialconjunctivitis. Am J Ophthalmol. 1991;112:2S–9S.
   Google Scholar
• Cohen EJ, Laibson PR, Arentsen JJ, Clemons CS. Corneal ulcers associated with cosmetic extended-wear soft contact lenses. Ophthalmology. 1987;94:109–114.
   PubMed Web of Science ®Google Scholar
• Ogawa GSH and Hyndiuk RA. Bacterial keratitis and conjunctivitis. In: Smolin G, Thoft RA (eds.) The cornea. Boston, MA, USA; Little, Brown and Co.; 1994:125–167.
   Google Scholar
• Fleiszig SMJ, Effron N, Pier GB. Extended contact lens wear enhances Pseudomonas aeruginosa adherence to human corneal epithelium. Invest Ophthalmol Vis Sci. 1992;33:2908–2916.
   Google Scholar
• Kernacki KA, Goebel DJ, Poosch MS, Hazlett LD. Early cytokine and chemokine gene expression during P. aeru-ginosa corneal infection in mice. Infect Immun. 1998; 66:376–379.
   PubMed Web of Science ®Google Scholar
• Steuhl KP, Doring G, Henni A, Thiel HJ, Botzenhart K. Relevance of host-derived and bacterial factors in P. aeruginosa corneal infections. Invest Ophthlamol Vis Sci. 1987;28:1559–1568.
   Google Scholar
• Goldberg JB, Ohman DE. Cloning and transcriptional regulation of the elastase lasA gene in mucoid and non-mucoid P. aeruginosa. J Bacteriol. 1986;169:1349–1351.
   Google Scholar
• Engel LS, Hill JM, Caballero AR, Green LC, O'Callaghan RJ. Protease IV, a unique extracellular protease and viru-lence factor from Pseudomonas aeruginosa. J Biol Chem. 1998;273:16792–16797.
   Google Scholar
• Baumann U, Wu S, Flaherty KM, McKay DB. Three-dimensional structure of the alkaline protease of Pseudo-monas aeruginosa: a two-domain protein with a calcium binding parallel beta roll motif EMBO. 1993;12:3357-3364.
   Google Scholar
• Guzzo J, Murgier M, Filloux A, Lazdunski A. Cloning of the Pseudomonas aeruginosa alkaline protease gene and secretion of the protease into the medium by Escheri-chia coli. J Bacteriol. 1990;172:942–948.
   PubMedGoogle Scholar
• Howe TR, Iglewski BH. Isolation and characterization of alkaline protease deficient mutants of Pseudomonas aeruginosa in vitro and in a mouse eye model. Infect Immun. 1984;43:1058–1063.
   PubMed Web of Science ®Google Scholar
• Nicas TI, Iglewski BH. The contribution of exoproducts to virulence of Pseudomonas aeruginosa. Can J Microbiol. 1985;31:387–392.
   PubMed Web of Science ®Google Scholar
• Kawaharajo K, Abe C, Homma JY, Kawano M, Gotoh E, Tanaka N, Morihara K. Corneal ulcers caused by pro-tease and elastase from Pseudomonas aeruginosa. Jpn J Exp Med. 1974;44:435–442.
   Google Scholar
• Kessler E, Kennan HE, Brown SI. Pseudomonas protease, purification, partial characterization, and its effect on collagen proteoglycan and rabbit corneas. Invest Opthal-mol Vis Sci. 1977;16:488–497.
   Google Scholar
• Kreger AS, Gray LD. Purification of Pseudomonas aeruginosa proteases and microscopic characterization of pseudomonal protease-induced rabbit corneal damage. Infect Immun. 1978;19:630–648.
   PubMed Web of Science ®Google Scholar
• Hong Y, Ghebrehiwet B. Effect of Pseudomonas aerugi-nosa elastase and alkaline protease on serum complement and isolated components C 1 q and C3. Clin Immun Immunopath. 1992;62:133–138.
   PubMedGoogle Scholar
• Parmely M, Gale A, Clabaugh M, Horvat R, Zhou WW. Proteolytic inactivation of cytokines by Pseudomonas aeruginosa. Infect Immun. 1990;58:3009–3014.
   Google Scholar
• Kharazmi A. Mechanisms involved in the evasion of the host defence by Pseudomonas aeruginosa. Immunol Let-ters. 1991;30:201–206.
   PubMedGoogle Scholar
• Twining SS, Kirschner SE, Mahnke LA, Frank DW. Effect of Pseudomonas aeruginosa elastase, alkaline protease, and exotoxin A on corneal proteinases and pro-teins. Invest Opthalmol Vis Sci. 1993;34:2699–2712.
   PubMed Web of Science ®Google Scholar
• Gupta SK, Masinick SA, Hobden JA, Berk RS, Hazlett LD. Bacterial proteases and adherence of Pseudomonas aeruginosa to mouse cornea. Exp Eye Res. 1996;62:641–650.
   Google Scholar
• Kernacki KA, Hobden JA, Hazlett LD, Fridman R, Berk RS. In vivo bacterial protease production during Pseudo-monas aeruginosa corneal infection. Invest Ophthalmol Vis Sci. 1995;36:1371–1378.
   Google Scholar
• Alionte LG, Estrellas Jr. PE, Hobden JA. A Pseudomo-nas aeruginosa strain isolated from a contact lens-induced acute red eye is elastase-deficient (Abstract). Invest Oph-thalmol Vis Sci. 1998;39(suppl.),145.
   Google Scholar
• Cowell BA, Willcox MDP, Hobden JA, Schneider RP, Tout S, Hazlett LD. An ocular strain of P. aeruginosa is inflammatory but not virulent in the scarified mouse model. Exp Eye Res. 1998;3:347–356.
   Google Scholar
• Pseudomonas Genome Project. 15 July 1999, revision date. [Online.] http://www.pseudomonas.com. 14 August 1999, last date accessed.
   Google Scholar
• Hazlett LD, Wells PA, Berk RS. Scanning electron mi-croscopy of the normal and experimentally infected ocular surface. Scanning Electron Microsc. 1984;3:1379–1389.
   Google Scholar
• Hazlett LD, Moon MM, Strejc M, Berk RS. Evidence for N-acetylmannosamine as an ocular receptor for P. aeruginosa adherence to scarified cornea. Invest Ophthal-mol Vis Sci. 1987;28:1978–1985.
   PubMed Web of Science ®Google Scholar
• Hobden JA, Masinick SA, Barrett RP, Hazlett LD. Proinflammatory cytokine deficiency and pathogenesis of Pseudomonas aeruginosa keratitis in aged mice. In-fect Immun. 1997;65:2754–2758.
   Web of Science ®Google Scholar
• Fleiszig SMJ, Zaidi TS, Fletcher EL, Preston MJ, Pier GB. Pseudomonas aeruginosa invades corneal epithelial cells during experimental infection. Infect Immun. 1994; 62:3485–3492.
   Google Scholar
• Fleiszig SMJ, Zaidi TS, Preston MJ, Grout M, Evans DJ, Pier GB. Relationship between cytotoxicity and corneal epithelial cell invasion by clinical isolates of Pseudomonas aeruginosa. Infect Immun. 1996;64:2288–2294.
   PubMed Web of Science ®Google Scholar
• Sambrook J, Fritsch EF, Maniatis T. In: Ford N, Noland C, Ferguson M, eds. Molecular cloning. Plainview, NY, USA; Cold Springs Harbor Laboratory Press; 1989.
   Google Scholar
• Schweizer HP and Hoang TT. An improved system for gene replacement and xylE fusion analysis in Pseudomo-nas aeruginosa. Gene. 1995;158:15–22.
   Google Scholar
• Diver JM, Bryan LE, Sokol PA. Transformation of Pseu-domonas aeruginosa by electroporation. Anal Biochem. 1990;189:75–79.
   Google Scholar
• Estrellas PS, Alione LG, Hobden JA. A Pseudomonas aeruginosa strain isolated from a Contact Lens-Induced Acute Red Eye (CLARE) is protease-deficient. Curr Eye Res. 2000;20:157–165.
   Google Scholar
• Roux, KH. Optimization and troubleshooting in PCR. In: Dieffenbach CW, Dveksler GS (eds.) PCR primer. Plainview, NY, USA; Cold Springs Harbor Laboratory Press; 1995:53–58.
   Google Scholar
• Gupta SK, Masinick SA, Garrett M, Hazlett LD. Pseudo-monas aeruginosa lipopolysaccharide binds galectin-3 and other human corneal epithelial proteins. Infect Immun. 1997;65:2747–2753 .
   PubMed Web of Science ®Google Scholar
• Singh A, Hazlett LD, Berk RS. Characterization of Pseudomonas aeruginosa adherence to mouse corneas in organ culture. Infect Immun. 1987;58:1301–1307.
   Google Scholar
• Kwon B, Hazlett LD. Association of CD4+ T cell-de-pendent keratitis with genetic susceptibility to Pseudomo-nas aeruginosa ocular infection. J Immun. 1997;159: 6283–6290.
   PubMed Web of Science ®Google Scholar
• Hobden JA, Masinick-McClellan SA, Barrett RP, Bark KS, Hazlett LD. Pseudomonas aeruginosa keratitis in knockout mice deficient in intercellular adhesion molecule 1. Infect Immun. 1999;67:972–975.
   Google Scholar
• Twining SS, Wilson PM, Manhke-Braam L, Loushin J. Characterization of an alternate form of Pseudomonas elastase that is synthesized by 85% of Pseudomonas aeruginosa corneal isolates (Abstract). Invest Ophthal-mol Vis Sci. 1998;39(suppl.),662.
   Google Scholar
• Kernacki KA, Berk RS. Characterization of arachidonic acid metabolism and the polymorphonuclear leukocyte response in mice infected intracorneally with Pseudomo-nas aeruginosa. Invest Ophthalmol Vis Sci. 1995 ;36: 16–23.
   PubMed Web of Science ®Google Scholar
• O'Callaghan RJ, Engle LS, Hobden JA, Callegan MC, Green LC, Hill JM. Pseudomonas keratitis: the role of an uncharacterized exoprotein, protease IV, in corneal virulence. Invest Ophthalmol Vis Sci. 1996;37:534–543.
   PubMed Web of Science ®Google Scholar
• Engel LS, Hill JM, Moreau JM, Green LC, Hobden JA, O'Callaghan RJ. Pseudomonas aeruginosa protease IV produces corneal damage and contributes to bacterial virulence. Invest Ophthalmol Vis Sci. 1998;39:662–665.
   Google Scholar
• Prentki P, Kirsch HM. In vitro insertional mutagenesis with a selectable DNA fragment. Gene. 1984;29:303–313.
   Google Scholar
• Duong F, Lazdunski A, Cami B, Murgier M. Sequence of a cluster of genes controlling synthesis and secretion of alkaline protease in Pseudomonas aeruginosa: relation-ships to other secretory pathways. Gene. 1992;121:47–54.
   PubMedGoogle Scholar