References
- Sack, R. A., Tan, K. O. and Tan, A. (1992) Diurnal tear cycle: evidence for a nocturnal inflammatory constitutive tear fluid. Invest. Ophthalmol. Vis. Sci. 33, 626–640.
- Tan, K. O., Sack, R. A., Holden, B. A. and Swarbrick, H. A. (1993) Temporal sequence of changes in tear film compo-sition during sleep. Curr. Eye Res. 12, 1001–1007.
- Janssen, P. T. and van Bijsterveld, O. P. (1983) Origin and biosynthesis of human tear fluid proteins. Invest. Ophthal-mol. Vis. Sci. 24, 623–630.
- Fullard, R. J. and Synder, C. (1990) Protein levels in non-stimulated and stimulated tears of normal human subjects. Invest. Ophthalmol. Vis. Sci. 31, 1119–1126.
- Maurice, D. M. (1973) The dynamics and drainage of tears. Internat. Ophthalmol. Clin. 13, 103–116.
- Doane, M. G. (1986) Tear spreading, turnover and drain-age. In The Preocular Tear Film in Health, Disease, and Contact Lens Wear (Ed. Holly, F. J.). Pp. 652–662. Dry Eye Institute, Lubbock, TX.
- Sack, R. A., Sathe, S., Hackworth, L. A., Willcox, M. D., Holden, B. A. and Morris, C. A. (1996) The effect of eye closure on protein and complement deposition on Group IV hydrogel contact lenses: relationship to tear flow dy-namics. Curr. Eye Res. 15, 1092–1100.
- Sullivan, D. A., Bloch, K. J. and Allensmith, M. R. (1984) Hormonal influence on the secretory immune system of the eye: androgen control of secretory component produc-tion by the rat exorbital gland. Immunology, 52, 234–246.
- Stolze, H. H. and Sommer, H. J. (1986) Effect of different secretagogues on rabbit lacrimal gland protein secretion. In The Preocular Tear Film in Health, Disease and Con-tact Lens Wear (Ed. Holly, F. J.). Pp. 409–415. Dry Eye Institute, Lubbock, TX.
- Sack, R. A., Underwood, P. A., Tan, K. O., Sutherland, H. and Morris, C. A. (1993) Vitronectin: possible contribution to the closed-eye external host-defense mechanism. Ocu-lar Immunol. Inflammation, 1, 327–336.
- Holden, B. A., Vannas, A., Sack, R. A. and Tan, A. (1991) Plasmin and complement C3 activation of inflammatory cells in the closed eye. (Abstract). Invest. Ophthalmol. Vis. Sci 32 (Suppl.), 732.
- Vannas, A., Sweeney, D. F., Holden, B. A., Sapyska, E., Salonen, E. M. and Vaheri, A. (1992) Tear plasmin activity with contact lens wear. Curr Eye Res. 11, 243–251.
- Wilson, G., O'Leary, D. J. and Holden, B. A. (1989) Cell content of tears following overnight wear of contact lens. Curr. Eye Res. 8, 329–335.
- Sakata, M., Sack, R. A., Sathe, S., Beaton, A., Lew, G. and Holden, B. A. (1996) Proteases and antiproteases in the closed eye tears. (Abstract). Invest. Ophthalmol. Vis. Sci 37 (Suppl.), S848.
- Kessing, S. V. (1966) Investigations of the conjunctival mucin: quantitative studies of the goblet cells of conjunc-tiva. Acta Ophthalmologica, 44, 439–453.
- Bogart, B. I., Taylor, T., Lew, G., Gaerian, P. A. and Den-ning, C. R. (1986) High-performance liquid chromato-graphic fractionation and partial characterization of cystic fibrosis serum ultrafiltrates. I Chromatogr. 381, 29–40.
- Fullard, R. J. (1988) Identification of proteins in small tear volumes with and without size exclusion HPLC fraction-ation. Curt: Eye Res. 7, 163–179.
- Baier, G., Wollensak, G., Mur, E., Redl, B., Stöffler, G. and Gottinger, W. (1990) Analysis of human tear proteins by different high-performance liquid chromatographic tech-niques. J. Chromatogr. 525, 319–328.
- Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680–685.
- Weber, K. and Osborn, M. (1969) The reliability of molec-ular weight determination by dodecyl sulfate-polyacryl-amide gel electrophoresis. I Biol. Chem. 244, 4406–4412.
- Davies, G. E. and Stark, G. R. (1970) Use of dimethyl suberimidate, a cross-linking reagent, in studying the sub-unit structure of oligomeric proteins. Proc. Natl. Acad. Sci USA, 66, 651–656.
- Fernandez-Patron, C., Castellanos-Serra, L. and Rod-riguez, P. (1992) Reverse staining of sodium dodecyl sul-fate polyacrylamide gels by imidazole-zinc salts: sensitive detection of unmodified proteins. Biotechniques, 12, 564–573.
- Wardi, A. H. and Michos, G. A. (1972) Alcian blue stain-ing of glycoproteins in acrylamide disc electrophoresis. Analytical Biochem. 49, 607–609.
- Dubray, G. and Bezard, G. (1982) A highly sensitive peri-odic acid-silver stain for 1,2-diol groups of glycoproteins and polysaccharides in polyacrylamide gels. Anal. Bio-chem. 119, 325–329.
- Stromqvist, M. and Gruffman, H. (1992) Periodic acid/ Schiff staining of glycoproteins immobilized on a blotting matrix. Biotechniques, 13, 744–746.
- Kuizenga, A., van Haeringen, N. J. and Kijlstra, A. (1991) Identification of lectin binding proteins in human tears. Invest. Ophthalmol. Vis. Sci. 32, 3277–3284.
- Bjerrum, K., Halken, P. and Prause, J. U. (1991) The nor-mal human tear glycoprotein profile detected with lectin probes. Exp. Eye Res. 53, 431–435.
- Reddy, M. S., Bobek, L. A., Haraszthy, G. G., Biesbrock, A. R. and Levine, M. J. (1992) Structural features of the low-molecular-mass human salivary mucin. Biochem. 287, 639–643.
- Cohen, R. E., Aguirre, A., Neiders, M. E., Levine, M. J., Jones, P. C., Reddy, M. S. and Haar, J. G. (1991) Immu-nochemistry and immunogenicity of low molecular weight human salivary mucin. Arch. Oral Biol. 36, 347–356.
- Gendler, S. J. and Spicer, A. P. (1995) Epithelial mucin genes. Review. Annu. Rev. PhysioL 57, 607–634.
- Burchell, J., Gendler, S., Taylor-Papadimitriou, J., Girling, A., Lewis, A., Mills, R. and Lamport, D. (1987) Develop-ment and characterization of breast cancer reactive mono-clonal antibodies directed to the core protein of the human milk mucin. Cancer Res. 47, 5476–5482.
- Girling, A., Bartkova, J., Burchell, J., Gendler, S., Gillett, C. and Taylor-Papadimitriou, J. (1989) A core protein epitope of the polymorphic epithelial mucin detected by the monoclonal antibody SM-3 is selectively exposed in a range of primary carcinomas. Int. J. Cancer, 43, 1072–1076.
- Baeckstrom, D., Nilsson, O., Price, M. R., Lindholm, L. and Hansson, G. C. (1993) Discrimination of MUC1 mucins from other sialyl-lea-carrying glycoproteins pro-duced by colon carcinoma cells using a novel monoclonal antibody. Cancer Res. 53, 755–761.
- Briggs, S., Price, M. R. and Tendler, S. J. (1991) Immune recognition of linear epitopes in peptide fragments of epi-thelial mucins. Immunology, 73, 505–507.
- Burchell, J. and Taylor-Papadimitriou, J. (1993) Effect of modification of carbohydrate side chains on the reactivity of antibodies with core-protein epitopes of the MUC1 gene product. Epith. Cell Biol. 2, 155–162.
- Dion, A. S., Williams, C. J., Herlyn, M. and Major, P. (1990) Human milk fat globule membrane glycoproteins express blood group-related determinants primarily on mucin-like epithelial membrane antigens and gp70. Bio-chem. International, 22, 295–302.
- Taylor-Papadimitriou, J. T., Peterson, J. A., Arklie, J., Burchell, J., Ceriani, R. L. and Bodmer, W. F. (1981) Monoclonal antibodies to epithelium-specific components of the human milk fat globule membrane: production and reaction with cells in culture. Int. J. Cancer, 28, 17–21.
- Woodward, M. P., Young, W. W., Jr. and Bloodgood, R. A. (1985) Detection of monoclonal antibodies specific for carbohydrate epitopes using periodate oxidation. J. Immu-noL Methods, 78, 143–153.
- Thornton, D. J., Howard, M., Devine, P. L. and Sheehan, J. K. (1995) Methods for separation and deglycosylation of mucin subunits. Analytical Biochem. 227, 162–167.
- Franklin, R. M., Prendegast, R. A. and Silverstein, A. M. (1979) Secretory immune system of rabbit ocular adnexa. Invest. Ophthalmol. Vis. Sci. 18, 1093–1096.
- Gillette, T. E., Allansmith, M. R., Griener, J. V. and Janusz, M. (1980) Histologic and immunohistologic comparison of main and accessory lacrimal tissue. Am. J. Ophthalmol. 89, 724–730.
- Cohen, E. J. and Allansmith, M. R. (1981) Fixation tech-niques for secretory component in human lacrimal gland and conjunctiva. Am. J. Ophthalmol. 91, 789–793.
- Berta, A. and Török, M. (1986) Soluble glycoproteins in aqueous tears. In The Preocular Tear Film in Health, Dis-ease and Contact Lens Wear (Ed. Holly, F. J.). Pp. 506–520. Dry Eye Institute, Lubbock, TX.
- Berta, A. (1986) Standardization of tear protein determina- tions: the effects of sampling, flow rate, and vascular per-meability. In The Preocular Tear Film in Health, Disease and Contact Lens Wear (Ed. Holly, F. J.). Pp. 418–433. Dry Eye Institute, Lubbock, TX.
- Moore, J. C. and Tiffany, J. M. (1979) Human ocular mucus: origins and preliminary characterization. Exp. Eye Res. 29, 291–301.
- Moore, J. C. and Tiffany, J. M. (1981) Human ocular mucous: chemical studies. Exp. Eye Res. 33, 203–212.
- Jay, G. D. and Hong, B. S. (1992) Characterization of a bovine synovial fluid lubricating factor: II. comparison with purified ocular and salivary mucin. Conn. Tissue Res. 28, 89–98.
- Dilly, P. N. (1985) Contribution of the epithelium to the stability of the tear film. Trans. Ophthalmol. Soc. UK, 104, 381–389.
- Bolis, S., Ball, M., Devine, P. and Morris, C. (1996) Mucin species in the human tear film. (Abstract). Invest. Ophthal-mol. Vis. Sci. 37 (Suppl.), S846.
- Jumblatt, M. M., Geoghegan, T. E. and Jumblatt, J. E. (1995) Mucin gene expression in human conjunctiva. (Abstract). Invest. Ophthalmol. Vis. Sci. 36 (Suppl.), S997.
- Inatomi, T., Spurr-Michaud, S., Tisdale, A. and Gipson, I. K. (1995) Human corneal and conjunctival epithelia express MUCI mucin. Invest. Ophthalmol. Vis. Sci. 36, 1818–1827.
- Than, Q., Spurr-Michaud, S., Tisdale, A. S., Inatomi, T. and Gipson, I. K. (1996) Use of fluorescence in situ hybridization to discern cellular distribution of mucin gene expression in conjunctival epithelium. (Abstract). Invest. Ophthalmol. Vis. Sci. 37 (Suppl.), S357.
- Inatomi, T., Spurr-Michaud, S., Tisdale, A., Feldman, S. T. and Gipson, I. K. (1996) Conjunctival epithelium ex-presses MUC4 and MUC5 mucins. (Abstract). Invest. Ophthalmol. Vis. Sci. 37 (Suppl.), S357.
- Watanabe, H., Fabricant, M., Tisdale, A. S., Spurr-Michaud, S. J., Lindberg, K. and Gipson, I. K. (1995) Human corneal and conjunctival epithelia produce a mucin-like glycoprotein for the apical surface. Invest. Ophthalmol. Vis. Sci 36, 337–344.
- Ligtenberg, M. J., Voss, H. L., Gennissen, A. M. and Hil-kens, J. (1990) Episialin, a carcinoma-associated mucin, is generated by a polymorphoric gene encoding splice vari-ants with alternative amino termini. J. Biol. Chem. 265, 5573–5578.
- Gendler, S., Taylor-Papadimitriou, J., Duhig, T., Rothbard, J. and Burchell, J. (1988) A highly immunogenic region of a human polymorphoric epithelial mucin expressed by car-cinomas is made up of tandem repeats. J. Biol. Chem. 263, 12820–12823.
- Baeckstrom, D., Karlsson, N. and Hansson, G. C. (1994) Purification and characterization of sialyl-Le(a)-carrying mucins of human bile: evidence for the presence of MUC1 and MUC3 apoproteins.Biol. Chem. 269, 14430–14437.
- Kim, K. C., Wasano, K., Niles, R. M., Schuster, J. E., Stone, P. J. and Brody, J. S. (1987) Human neutrophil elastase releases cell surface mucins from primary cultures of hamster tracheal epithelial cells. Proc. Natl. Acad. Sci. USA, 84, 9304–9308.
- Shimizu, Y. and Shaw, S. (1993) Cell adhesions: mucins in the mainstream. Nature, 366, 630–631.