Corneal epithelial wound healing increases the expression but not long lasting activation of the p85a subunit of phosphatidylinositol-3 kinase

References

• Kenyon, K. R., Chaves, H. V. (1994) Neurophysiology and pathologic response of corneal and conjunctival dis-ease. In Smolin, G., Thoft, R. A., eds. iThe Cornea,i iLit-tle,i iBrown and Co,i iUSA,i 69–111.
   Google Scholar
• Bazan, H. E. P., Birkle, D. L., Beuerman, R., Bazan, N. G. (1985) Inflammation-induced stimulation of the syn-thesis of prostaglandins and lipoxygenase-reaction prod-ucts in rabbit cornea. iCurr. Eye Res.i b4,b 175–179.
   Google Scholar
• Bazan, H. E. P. (1987) Corneal injury alters eicosanoid formation in the rabbit anterior segment iin vivo. Invest. Ophthalmol. Vis. Sci.i b28,b 314–319.
   Google Scholar
• Bazan, H. E. P., Reddy, S. T. K., Lin, N. (1991) Plate-let-activating factor (PAF) accumulation correlates with injury in the cornea. iExp. Eye Res.i b52,b 481–491
   Google Scholar
• Bazan, H. E. P., Varner, L. (1997) A mitogen-activated protein kinase (MAP-kinase) cascade is stimulated by platelet-activating factor (PAF) in corneal epithelium. iCurr. Eye Res.i b16,b 372–379.
   Google Scholar
• Lin, N., Bazan, H. E. P. (1992) Protein kinase C sub-species in rabbit corneal epithelium: Increased activity of a subspecies during wound healing. iCurr. Eye Res.i b11,b 899–907.
   Google Scholar
• Chandrasekher, G., Bazan, N. G., Bazan, H. E. P. Se- lective changes in protein kinase (PKC) isoform expres-sion in rabbit corneal epithelium during wound healing. Inhibition of corneal epithelial repair by PKCa antisense. iExp. Eye Res.i (in press).
   Google Scholar
• Li, Q., Weng, J., Mohan, R. R., Bennett, G., Schwall, R., Wang, Z., Tabor, K., Kim, J., Hargrave, S., Cuevas, K. H., Wilson, S. E. (1996) Hepatocyte growth factor and hepatocyte growth factor receptor in the lacrimal gland, tears, and cornea. iInvest. Ophthal. Vis. Sci.i b37,b 727–739.
   Google Scholar
• Sheardown, H., Cheng, Y. (1996) Tear EGF concentra-tion following corneal epithelial wound creation. iJ. Ocul. Pharmacol. Therap.i b12,b 239–243.
   Google Scholar
• Zieske, J. D., Wasson, M. (1993) Regional variation in distribution of EGF receptor in developing and adult corneal epithelium. iJ. Cell. Sci.i b106,b 145–152.
   Google Scholar
• Wilson, S. E., Walker, J. W., Chwang, E. L., He, Y. (1993) Hepatocyte growth factor, keratinocyte growth factor, their receptors, fibroblast growth factor receptor-2, and the cells of the cornea. iInvest. Ophthal. Vis. Sci.i b34,b 2544–2561.
   Google Scholar
• Li, D. Q., Tseng, S. C. (1996) Differential regulation of cytokine and receptor transcript expression in human cor-neal and limbal fibroblasts by epidermal growth factor, transforming growth factor-a, platelet-derived growth factor b, and interleukin-li3. iInvest. Ophthal. Vis. Sci.i b37,b 2068–2080.
   PubMedGoogle Scholar
• Carpenter, C. L., Cantley, L. C. (1996) Phosphoinosi-tide kinases. iCurr. Opinion. Cell. Biol.i b8,b 153–158.
   Google Scholar
• Stephens, L. R., Jackson, T. R., Hawkins, P. T. (1993) Agonist-stimulated synthesis of phosphatidylinositol (3, 4, 5)-trisphosphate: a new intracellular signaling system? iBiochem. Biophys. Acta.i b1179,b 27–75.
   Google Scholar
• Domin, J., Waterfield, M. D. (1997) Using structure to define the function of phosphoinositide 3- kinase fami-ly members. iFEBS Lett.i b410,b 91–95.
   Google Scholar
• Keith, C. T., Schreiber, S. L. (1995) PIK-related kina-ses: DNA repair, recombination, and cell cycle check-points. iScience.i b270,b 50–51.
   Google Scholar
• DeCamilli, P., Emr, S. D., McPherson, P. S., Novick, P. (1996) Phosphoinositides as regulators in membrane traf-fic. iScience.i b271,b 1533–1538.
   Google Scholar
• Varticovski, L., Findik, D. H., Keeler, M. L., Susa, M. (1994) Role of PI 3-kinase in mitogenesis. iBiochem. Biophys. Acta.i b1226,b 1–11.
   Google Scholar
• Bazan, H. E. P., Dobard, P., Reddy, S. T. K. (1987) Calcium- and phospholipid-dependent protein kinase C and phosphatidylinositol kinase: two major phosphory-lation systems in the cornea. iCurr. Eye Res.i b6,b 667–673.
   Google Scholar
• Zhang, Y., Akhtar, R. A. (1996) Effect of epidermal growth factor on phosphatidylinositol 3-kinase activity in rabbit corneal epithelial cells. iExp. Eye Res.i b63,b 265–275.
   Google Scholar
• Lees, M. B., Paxman, S. (1972) Modification of the Lowry procedure for the analysis of proteolipid protein. iAnal. Biochem.i b47,b 184–192.
   Google Scholar
• Clarke, N. G., Dawson, R. M. C. (1981) Alkaline-ON-transacylation. iBiochem. J.i b195,b 301–306.
   Google Scholar
• Auger, K. R., Serunian, L. A., Soltoff, S. P., Libby, P. and Cantley, L. C. (1989) PDGF-dependent tyrosine phos-phorylation stimulates production of novel polyphospho-inositides in intact cells. iCell.i b57,b 167–175.
   Google Scholar
• Kanagasundaram, V., Jaworowski, A., Hamilton, J. A. (1996) Association between phosphatidyl- inosito1-3 ki-nase, Cbl and other tyrosine phosphorylated proteins in colony-stimulating factor-1 stimulated macrophages. iBiochem. J.i b320,b 69–77.
   Google Scholar
• Hayashi, H., Kamohara, S., Nishioka, Y., Kanai, F., Miyake, N., Fukui, Y., Shibasaki, F., Takenawa, T., Ebina, Y. (1992) Insulin treatment stimulates the tyrosine phos-phorylation of the a-type 85-kDa subunit of phosphati-dylinositol 3-kinase iin vivo. J. Biol. Chem.i b267,b 22575–22580 .
   Google Scholar
• Kimura, K., Hattori, S., Kabuyama, Y., Shizawa, Y., Takayanag, J., Nakamura, S., Toki, S., Matsuda, Y., Onodera, K., Fukui, Y. (1994) Neurite outgrowth of PC12 cells is suppressed by wortmannin, a specific inhibitor of phosphatidylinositol 3-kinase. iJ. Biol. Chem.i b269,b 18961–18967.
   Google Scholar
• Vlahos, C. J., Matter, W. F., Hui, K. Y., Brown, R. F. (1994) A specific inhibitor of phosphatidylinositol 3- ki-nase, 2-(4-morpholiny1)-8-phenyl-4H-1-benzopyran-4-one (LY294002). iJ. Biol. Chem.i b269,b 5241–5248.
   Google Scholar
• Zieske, J. D., Gipson, I. K. (1986) Protein synthesis during corneal epithelial wound healing. iInvest. Ophthalmol. Vis. Sci.i b27,b 1–7.
   Google Scholar
• Pfister, R. R. (1975) The healing of corneal epithelial abrasions in the rabbit: a scanning electron microscope study. iInvest Ophthal. Vis. Sci.i b14,b 648–661.
   Google Scholar
• Khaw, P. T., Schultz, G. S., MacKay, S. L. D., Chegini, N., Adams, J., Shimizu, R. (1992) Human corneal epi-thelium: autocrine production of TGFa mRNA and pro-tein. iInvest. Ophthalmol. Vis. Sci.i b33,b 3302–3306.
   Google Scholar
• Ohashi, Y., Motokura, M., Kinoshita, Y., Mano, T., Watanabe, H., Kinoshita, S., Manabe, R., Oshiden, K., Yanaihara, C. (1989) Presence of human epidermal growth factor in human tears. iInvest. Ophthalmol. Vis. Sci.i b30,b 1879–1889.
   Google Scholar
• Zhang, Y., Akhtar, R. A. (1997) Epidermal growth fac-tor stimulation of phosphatidylinositol 3-kinase during wound closure in rabbit corneal epithelial cells. iInvest. Ophthalmol. Vis. Sci.i b38,b 1139–1148.
   Google Scholar
• Wilson, S. E., He, Y., Weng, J., Zieske, J. D., Jester, J.V., Schultz, G. S. (1994) Effect of epidermal growth factor, hepatocyte growth factor, and keratinocyte growth factor, on proliferation, motility and differentiation of human corneal epithelial cells. iExp. Eye Res.i b59,b 665–678.
   Google Scholar
• Westphal, R. S., Anderson, K. A., Means, A. R., Wad-zinski, B. E. (1998) A signaling complex of Ca2+- Cal-modulin-dependent protein kinase IV and protein phos-phatase 2A. iScience,i b280,b 1258–1261.
   Google Scholar
• Carpenter, C. L., Auger, K. R., Duckworth, B. C., Hou,W., Schaffhausen, B., Cantley, L. C. (1993) A tightly associated serine/threonine protein kinase regulates phos-phoinositide 3-kinase activity. iMol. Cell. Biol.i b13,b 1657–1665.
   Google Scholar
• Giorgino, F., Pedrini, M. T., Matera, L., Smith, R. J. (1997) Specific increase in p85a expression in response to dexamethasone is associated with inhibition of insu-lin-like growth factor-I stimulated phosphatidylinositol 3-kinase activity in cultured muscle cells. iJ. Biol. Chem.i b272,b 7455–7463.
   Google Scholar
• Bennett, N. T., Schultz, G. S. (1993) Growth factors and wound healing. II. Role in normal and chronic wound healing. iAm. J. Surg.i b166,b 74–81.
   Google Scholar
• Schultz, G., Khaw, P. T., Oxford, K., Macauley, S., van Setten, G., Chegini, N. (1994) Growth factors and ocu-lar wound healing. iEye,i b8,b 184–187.
   Google Scholar
• Toker, A., Meyer, M., Reddy, K. K., Falck, J. R., Ane-ja, R., Aneja, S., Parra, A., Burns, D. J., Ballas, L. M., Cantley, L. C. (1994) Activation of protein kinase C family members by the novel polyphosphoinositides PI-3,4 P2 and PI-3,4,5-P3. iJ. Biol. Chem.i b269,b 32358–32367.
   Google Scholar
• Derman, M. P., Toker, A., Hartwig, J. H., Spokes, K., Falck, J. R., Chen, C., Cantley, L. C., Cantley, L. G. (1997) The lipid products of phosphoinositide 3-kinase increase cell motility through protein kinase C. iJ. Biol. Chem.i b272,b 6465–6470.
   Google Scholar
• Borner, C., Ueffing, M., Jaken, S., Parker, P. J., Wein-stein, I. B. (1995) Two closely related isoforms of pro-tein kinase C produce reciprocal effects on the growth of rat fibroblasts. iJ. Biol. Chem.i b270,b 78–86.
   Google Scholar
• Seedford, K., Shearaman, M., Ulbrich, A. (1995) Rapid and long term effects of protein kinase C on receptor tyrosine kinase phosphorylation and degradation. I iBiol. Chem.i b32,b 18953–18960.
   Google Scholar