In vitro treatment of Enterococcus faecalis with calcium hydroxide impairs phagocytosis by human macrophages

References

• Fujii R, Saito Y, Tokura Y, et al. Characterization of bacterial flora in persistent apical periodontitis lesions. Oral Microbiol Immunol. 2009;24:502–505.
   PubMedGoogle Scholar
• Kayaoglu G, Orstavik D. Virulence factors of Enterococcus faecalis: relationship to endodontic disease. Crit Rev Oral Biol Med. 2004;15:308–320.
   PubMedGoogle Scholar
• Wang QQ, Zhang CF, Chu CH, et al. Prevalence of Enterococcus faecalis in saliva and filled root canals of teeth associated with apical periodontitis. Int J Oral Sci. 2012;4:19–23.
   PubMed Web of Science ®Google Scholar
• Pereira RS, Rodrigues VAA, Furtado WT1, et al. Microbial analysis of root canal and periradicular lesion associated to teeth with endodontic failure. Anaerobe. 2017;48:12–18.
   PubMed Web of Science ®Google Scholar
• Zou J, Shankar N. The opportunistic pathogen Enterococcus faecalis resists phagosome acidification and autophagy to promote intracellular survival in macrophages. Cell Microbiol. 2016;18:831–843.
   PubMed Web of Science ®Google Scholar
• Kampfer J, Gohring TN, Attin T, et al. Leakage of food-borne Enterococcus faecalis through temporary fillings in a simulated oral environment. Int Endod J. 2007;40:471–477.
   PubMed Web of Science ®Google Scholar
• Razavi A, Gmur R, Imfeld T, et al. Recovery of Enterococcus faecalis from cheese in the oral cavity of healthy subjects. Oral Microbiol Immunol. 2007;22:248–251.
   PubMedGoogle Scholar
• Vidana R, Rashid MU, Ozenci V, et al. The origin of endodontic Enterococcus faecalis explored by comparison of virulence factor patterns and antibiotic resistance to that of isolates from stool samples, blood cultures and food. Int Endod J. 2016;49:343–351.
   PubMed Web of Science ®Google Scholar
• Baik JE, Ryu YH, Han JY, et al. Lipoteichoic acid partially contributes to the inflammatory responses to Enterococcus faecalis. J Endod. 2008;34:975–982.
   PubMed Web of Science ®Google Scholar
• Mohammadi Z, Shalavi S, Yazdizadeh M. Antimicrobial activity of calcium hydroxide in endodontics: a review. Chonnam Med J. 2012;48:133–140.
   PubMedGoogle Scholar
• Baik JE, Jang KS, Kang SS, et al. Calcium hydroxide inactivates lipoteichoic acid from Enterococcus faecalis through deacylation of the lipid moiety. J Endod. 2011;37:191–196.
   PubMed Web of Science ®Google Scholar
• Evans M, Davies JK, Sundqvist G, et al. Mechanisms involved in the resistance of Enterococcus faecalis to calcium hydroxide. Int Endod J. 2002;35:221–228.
   PubMed Web of Science ®Google Scholar
• McHugh CP, Zhang P, Michalek S, et al. pH required to kill Enterococcus faecalis in vitro. J Endod. 2004;30:218–219.
   PubMed Web of Science ®Google Scholar
• Zoletti GO, Pereira EM, Schuenck RP, et al. Characterization of virulence factors and clonal diversity of Enterococcus faecalis isolates from treated dental root canals. Res Microbiol. 2011;162:151–158.
   PubMed Web of Science ®Google Scholar
• Kopp W, Schwarting R. Differentiation of T lymphocyte subpopulations, macrophages, and HLA-DR-restricted cells of apical granulation tissue. J Endod. 1989;15:72–75.
   PubMed Web of Science ®Google Scholar
• Omoregie FO, Ojo MA, Saheeb B, et al. Periapical granuloma associated with extracted teeth. Niger J Clin Pract. 2011;14:293–296.
   PubMed Web of Science ®Google Scholar
• Cortes-Perez NG, Dumoulin R, Gaubert S, et al. Overexpression of Enterococcus faecalis elr operon protects from phagocytosis. BMC Microbiol. 2015;15:112.
   PubMed Web of Science ®Google Scholar
• Bogdan C. Nitric oxide synthase in innate and adaptive immunity: an update. Trends Immunol. 2015;36:161–178.
   PubMed Web of Science ®Google Scholar
• Prolo C, Alvarez MN, Radi R. Peroxynitrite, a potent macrophage-derived oxidizing cytotoxin to combat invading pathogens. Biofactors. 2014;40:215–225.
   PubMed Web of Science ®Google Scholar
• Weiss G, Schaible UE. Macrophage defense mechanisms against intracellular bacteria. Immunol Rev. 2015;264:182–203.
   PubMed Web of Science ®Google Scholar
• Korhonen R, Lahti A, Kankaanranta H, et al. Nitric oxide production and signaling in inflammation. Curr Drug Targets Inflamm Allergy. 2005;4:471–479.
   PubMedGoogle Scholar
• Guzik TJ, Korbut R, Adamek-Guzik T. Nitric oxide and superoxide in inflammation and immune regulation. J Physiol Pharmacol. 2003;54:469–487.
   PubMed Web of Science ®Google Scholar
• Shepard BD, Gilmore MS. Differential expression of virulence-related genes in Enterococcus faecalis in response to biological cues in serum and urine. Infect Immun. 2002;70:4344–4352.
   PubMed Web of Science ®Google Scholar
• Chavez de Paz LE. Redefining the persistent infection in root canals: possible role of biofilm communities. J Endod. 2007;33:652–662.
   PubMed Web of Science ®Google Scholar
• Burley KM, Sedgley CM. CRISPR-Cas, a prokaryotic adaptive immune system, in endodontic, oral, and multidrug-resistant hospital-acquired Enterococcus faecalis. J Endod. 2012;38:1511–1515.
   PubMed Web of Science ®Google Scholar
• Penas PP, Mayer MP, Gomes BP, et al. Analysis of genetic lineages and their correlation with virulence genes in Enterococcus faecalis clinical isolates from root canal and systemic infections. J Endod. 2013;39:858–864.
   PubMed Web of Science ®Google Scholar
• Ferreira FB, Campos Rabang HR, Pinheiro ET, et al. Root canal microbiota of dogs’ teeth with periapical lesions induced by two different methods. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006;102:564–570.
   PubMed Web of Science ®Google Scholar
• Sedgley CM, Nagel AC, Shelburne CE, et al. Quantitative real-time PCR detection of oral Enterococcus faecalis in humans. Arch Oral Biol. 2005;50:575–583.
   PubMed Web of Science ®Google Scholar
• Pinke KH, Freitas P, Viera NA, et al. Decreased production of proinflammatory cytokines by monocytes from individuals presenting Candida-associated denture stomatitis. Cytokine. 2016;77:145–151.
   PubMed Web of Science ®Google Scholar
• Lima HG, Pinke KH, Gardizani TP, et al. Mast cells act as phagocytes against the periodontopathogen Aggregatibacter actinomycetemcomitans. J Periodontol. 2013;84:265–272.
   PubMed Web of Science ®Google Scholar
• Gardizani TP, Pinke KH, Lima HG, et al. Phagocytosis and nitric oxide production by peritoneal adherent cells in response to Candida albicans in aging: a collaboration to elucidate the pathogenesis of denture stomatitis. J Appl Oral Sci. 2017;25:265–273.
   PubMed Web of Science ®Google Scholar
• Hed J. The extinction of fluorescence by crystal violet and its use to differentiate between attached and ingested microorganisms in phagocytosis. FEMS Microbiol Lett. 1977;1:5.
   Web of Science ®Google Scholar
• Akbari T, Pourhajibagher M, Hosseini F, et al. The effect of indocyanine green loaded on a novel nano-graphene oxide for high performance of photodynamic therapy against Enterococcus faecalis. Photodiagnosis Photodyn Ther. 2017;20:148–153.
   PubMed Web of Science ®Google Scholar
• Bohora AA, Kokate SR. Good bugs vs bad bugs: evaluation of inhibitory effect of selected probiotics against Enterococcus faecalis. J Contemp Dent Pract. 2017;1;18:312–316.
   Google Scholar
• Tonea A, Badea M, Oana L, et al. Antibacterial and antifungal activity of endodontic intracanal medications. Clujul Med. 2017;90:344–347.
   PubMedGoogle Scholar
• Eaton TJ, Gasson MJ. Molecular screening of Enterococcus virulence determinants and potential for genetic exchange between food and medical isolates. Appl Environ Microbiol. 2001;67:1628–1635.
   PubMed Web of Science ®Google Scholar
• Franz CM, Muscholl-Silberhorn AB, Yousif NM, et al. Incidence of virulence factors and antibiotic resistance among Enterococci isolated from food. Appl Environ Microbiol. 2001;67:4385–4389.
   PubMed Web of Science ®Google Scholar
• Sabatino R, Di Cesare A, Pasquaroli S, et al. Adherence and intracellular survival within human macrophages of Enterococcus faecalis isolates from coastal marine sediment. Microbes Infect. 2015;17:660–664.
   PubMed Web of Science ®Google Scholar
• Pinke KH, de Lima HG, Cunha FQ, et al. Mast cells phagocyte Candida albicans and produce nitric oxide by mechanisms involving TLR2 and Dectin-1. Immunobiology. 2016;221:220–227.
   PubMed Web of Science ®Google Scholar
• Appelbe OK, Sedgley CM. Effects of prolonged exposure to alkaline pH on Enterococcus faecalis survival and specific gene transcripts. Oral Microbiol Immunol. 2007;22:169.
   PubMedGoogle Scholar
• Ran S, Gu S, Wang J, et al. Dentin tubule invasion by Enterococcus faecalis under stress conditions ex vivo. Eur J Oral Sci. 2015;123:362–368.
   PubMed Web of Science ®Google Scholar
• Ran S, Liu B, Jiang W, et al. Transcriptome analysis of Enterococcus faecalis in response to alkaline stress. Front Microbiol. 2015;6:795.
   PubMed Web of Science ®Google Scholar
• Flahaut S, Hartke A, Giard JC, et al. Alkaline stress response in Enterococcus faecalis: adaptation, cross-protection, and changes in protein synthesis. Appl Environ Microbiol. 1997;63:812–814.
   PubMed Web of Science ®Google Scholar
• Uribe-Querol E, Rosales C. Control of phagocytosis by microbial pathogens. Front Immunol. 2017;8:1368.
   PubMed Web of Science ®Google Scholar
• Kaufmann SHE, Dorhoi A. Molecular determinants in phagocyte-bacteria interactions. Immunity. 2016;44:476–491.
   PubMed Web of Science ®Google Scholar
• Graunaite I, Lodiene G, Maciulskiene V. Pathogenesis of apical periodontitis: a literature review. J Oral Maxillofac Res. 2012;2:e1.
   PubMedGoogle Scholar
• Sánchez-Sanhueza G, González-Rocha G, Dominguez M. Enterococcus spp. isolated from root canals with persistent chronic apical periodontitis in a Chilean population. Braz J Oral Sci. 2015;14:241–245.
   Google Scholar
• Love RM. Enterococcus faecalis - a mechanism for its role in endodontic failure. Int Endod J. 2001;34:399–405.
   PubMed Web of Science ®Google Scholar