178
Views
9
CrossRef citations to date
0
Altmetric
Bioanalytical

Continuous‐Flow PCR Microfluidics for Rapid DNA Amplification Using Thin Film Heater with Low Thermal Mass

, &
Pages 1672-1685 | Received 22 Aug 2006, Accepted 01 Dec 2006, Published online: 25 Oct 2011

References

  • Chiou , J. , Matsudaira , P. , Sonin , A. and Ehrlich , D. 2001 . A closed‐cycle capillary polymerase chain reaction machine . Anal. Chem. , 73 ( 9 ) : 2018 – 2021 .
  • Curcio , M. and Roeraade , J. 2003 . Continuous segmented‐flow polymerase chain reaction for high‐throughput miniaturized DNA amplification . Anal. Chem. , 75 ( 1 ) : 1 – 7 .
  • Dorfman , K. D. , Chabert , M. , Codarbox , J. H. , Rousseau , G. , de Cremoux , P. and Viovy , J.‐L. 2005 . Contamination‐free continuous flow microfluidic polymerase chain reaction for quantitative and clinical applications . Anal. Chem. , 77 : 3700 – 3704 .
  • Erill , I. , Campoy , S. , Erill , N. , Barbé , J. and Aguiló , J. 2003 . Biochemical analysis and optimization of inhibition and adsorption phenomena in glass‐silicon PCR chips . Sens. Actuators B , 96 : 685 – 692 .
  • Kopp , M. U. , de Mello , A. J. and Manz , A. 1998 . Chemical amplification: Continuous‐flow PCR on a chip . Science , 280 : 1046 – 1048 .
  • Nakano , H. , Matsuda , K. , Yohda , M. , Nagamune , T. , Endo , I. and Yamane , T. 1994 . High‐speed polymerase chain‐reaction in constant flow . Biosci. Biotechnol. Biochem. , 58 : 349 – 352 .
  • Northrup , M. A. , Ching , M. T. , White , R. M. and Watson , R. T. DNA amplification in a microfabricated reaction chamber . Tranducer'93 Seventh International Conference on Solid State Sensors and Actuators . pp. 924 – 926 . Yokohama, , Japan ISBN 4‐9900247‐2‐9
  • Park , N. , Kim , S. and Hahn , J. H. 2003 . Cylindrical compact thermal‐cycling device for continuous‐flow polymerase chain reaction . Anal. Chem. , 75 ( 21 ) : 6029 – 6033 .
  • Saiki , R. K. , Scharf , S. , Faloona , F. , Mullis , K. B. , Horn , G. T. , Erlich , H. A. and Arnheim , N. 1985 . Enzymatic amplification of β‐globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia . Science , 230 : 1350 – 1354 .
  • Schneegaβ , I. , Bräutigam , R. and Köhler , J. M. 2001 . Miniaturized flow‐through PCR with different template types in a silicon chip thermocycler . Lab Chip. , 1 : 42 – 49 .
  • Zhang , C. S. , Xu , J. L. , Ma , W. L. and Zheng , W. L. 2006a . PCR microfluidic devices for DNA amplification . Biotechnol. Adv. , 24 ( 3 ) : 243 – 284 .
  • Zhang , C. S. , Xu , J. L. , Wang , J. Q. and Wang , H. P. 2007 . Continuous‐flow polymerase chain reaction microfluidics by using spiral capillary channel embedded on copper . Anal. Lett. , 40 : 497 – 511 .

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.