175
Views
5
CrossRef citations to date
0
Altmetric
BIOANALYTICAL

Determination of S-Adenosylmethionine and S-Adenosylhomocysteine from Human Blood Samples by HPLC-FL

, , &
Pages 1720-1731 | Received 04 Mar 2008, Accepted 31 Mar 2008, Published online: 16 Jun 2010

REFERENCES

  • Ansorena , E. , Berasain , C. , Lopez Zabalza , M.J. , Avila , M.A. , Garcia-Trevijano , E.R. , and Iraburu , M.J. 2006 . Differential regulation of the JNK/AP-1 pathway by S-adenosylmethionine and methylthioadenosine in primary rat hepatocytes versus HuH7 hepatoma cells . Am. J. Physiol. Gastrointest. Liver Physiol. , 290 : 1186 – 1193 .
  • Birsan , C. , Litescu , S.C. , Vasilescu , I. , Eremia , S. , and Radu , G.L. 2006 . Aplicability study on some HPLC methods for simultaneous determination of SAM and SAH . Rev. de Chimie. , 57 : 790 – 795 .
  • Bottiglieri T. 2002 . S-Adenosyl-L-methionine (SAMe): from the bench to the bedside—molecular basis of a pleiotrophic molecule . Am. J. Clin. Nutr. , 76 : 1151 – 1157 .
  • Bressa G.M. 1994 . S-adenosyl-l-methionine (SAMe) as antidepressant: meta-analysis of clinical studies . Acta Neurol. Scand. , 154 : 7 – 14 .
  • Capdevila , A. and Wagner , C. 1998 . Measurement of plasma S-adenosylmethionine and S-adenosylhomocysteine as their fluorescent isoindoles . Anal. Biochem. , 264 : 180 – 184 .
  • Castro , R. , Struys , E.A. , Jansen , E.E. , Blom , H.J. , De Almeida , I.T. , and Jakobs , C. 2002 . Quantification of plasma S-adenosylmethionine and S-adenosylhomocysteine as their fluorescent 1,N6-etheno derivatives: An adaptation of previously described methodology . J. Pharm. Biomed. Anal. , 29 : 963 – 968 .
  • Chiang , P.K. , Gorinn , R.K. , Tal , J. , Zeng , G.C. , Doctor , B.P. , Pardhasaradhi , D. , and McCanin , P.P. 1996 . S-adenosylmethionine and methylation . Faseb. J. , 10 : 471 – 480 .
  • Gigliozzi , A. , Romeo , R. , Fraioli , F. , Cantafora , A. , Delle Monache , M. , Cardilli , A. , Attili , A.F. , Scafato , E. , Carli , L. , and Alvaro , D. 1998 . Effect of S-adenosyl-L-methionine and dilinoleoylphosphatidylcholine on liver lipid composition and ethanol hepatotoxicity in isolated perfused rat liver . Dig. Dis. Sci. , 43 : 2211 – 2222 .
  • Grillo , M.A. and Colombatto , S. 2005 . S-adenosylmethionine and protein methylation . Amino Acids , 28 : 357 – 362 .
  • Halsted , C.H. , Villanueva , J.A. , Devlin , A.M. , Niemela , O. , Parkkila , S. , Garrow , T.A. , Wallock , L.M. , Shigenaga , M.K. , Melnyk , S. , and James , S.J. 2002. Folate deficiency disturbs hepatic methionine metabolism and promotes liver injury in the ethanol-fed micropig. Proc. Natl. Acad. Sci. USA , 99: 10072–10077.
  • Herbig K. , Chiang , E.P. , Lee , L.R. , Hills , J. , Shane , B. , and Stover , P.J. 2002 . Cytoplasmic serine hydroxymethyltransferase mediates competition between folate-dependent deoxyribonucleotide and S-adenosylmethionine Biosyntheses . J. Biol. Chem. , 277 : 38381 – 38389 .
  • James , S.J. , Cutler , P. , Melnyk , S. , Jernigan , S. , Janak , L. , Gaylor , D.W. , and Neubrander , J.A. 2004 . Metabolic biomarkers of increased oxidative stress and impaired methylation capacity in children with autism . Am. J. Clin. Nutr. , 80 : 1611 – 1617 .
  • Kozbial , P.Z. and Mushegian , A.R. 2005 . Natural history of S-adenosylmethionine-binding proteins . BMC Struct. Biol. 5 : 19 .
  • Lehman-Mckeeman , L.D. , Gamsky , E.A. Hicks , S.M. Vassallo , J.D. , Mar , M.H. , and Zeisel , S.H. 2002 . Diethanolamine induces hepatic choline deficiency in mice . Toxicol. Sci . 67 : 38 – 45 .
  • Loehrer , F.M. , Angst , C.P. , Brunner , F.P. , Haefeli , W.E. , and Fowler , B. 1998 . Evidence for disturbed S-adenosylmethionine: S-adenosylhomocysteine ratio in patients with end-stage renal failure: A cause for disturbed methylation reactions? Nephrol. Dial. Transplant ., 13 : 656 – 661 .
  • Lu , S.C. , Huang , Z.Z. , Yang , H. , Mato , J.M. , Avila , M.A. , and Tsukamotoh , H. 2000 . Changes in methionine adenosyltransferase and S-adenosylmethionine homeostasis in alcoholic rat liver . Am. J. Physiol. Gastrointest. Liver Physiol . 279 : 178 – 185 .
  • Luippold , G. , Delabar , U. , Kloor , D. , and Muhlbauer , B. 1999 . Simultaneous determination of adenosine, S-adenosylhomocysteine and S-adenosylmethionine in biological samples using solid-phase extraction and high-performance liquid chromatography . J. Chromatogr. B , 724 : 231 – 238 .
  • Lunn , G. and Hellwing , L.C. 1998 . Handbook of Derivatization Reactions for HPLC . New York : John Wiley & Sons Inc .
  • Melnyk , S. , Pogribna , M. , Pogrinbny , I.P. Yi , P. , and James , S.J. 2000 . Measurement of Plasma and Intracellular S-Adenosylmethionine and S-adenosylhomocysteine utilizing coulometric electrochemical detection: alterations with plasma homocysteine and pyridoxal 59-phosphate concentrations . Clin. Chem. , 46 : 265 – 272 .
  • Morrison , L.D. , Smith , D.D. , and Kish , S.J. 1996 . Brain S-adenosylmethionine levels are severely decreased in Alzheimer' s Disease . J. Neurochem . 67 : 1328 – 1331 .
  • Pogribna , M. , Melnyk , S. , Pogribny , I. , Chango , A. , Yi , P. , and James , S.J. 2001 . Homocysteine metabolism in children with down syndrome: in vitro modulation . Am. J. Hum. Genet. , 69 : 88 – 95 .
  • Saletu , B. , Anderer , P. , DiPadova , C. , Assandri , A. , and Saletu-Zyhlarz , G.M. 2002 . Electrophysiological neuroimaging of the central effects of S-adenosyl-L-methionine by mapping of electroencephalograms and event-related potentials and low-resolution brain electromagnetic tomography . Am. J. Clin. Nutr . 76 : 1162S – 1171S .
  • Struys , E. A, Jansen , E.E. , De Meer , K. , and Jakobs , C. 2000 . Determination of S-adenosylmethionine and S-adenosylhomocysteine in plasma and cerebrospinal fluid by stable-isotope dilution tandem mass spectrometry . Clin. Chem. , 46 : 1650 – 1656 .
  • Ubeda , N. , Alonso-Aperte , E. , and Varela-Moreiras , G. 2002 . Acute valproate administration impairs methionine metabolism in rats . J. Nutr. , 132 : 2737 – 2742 .
  • Wise , C.K. , Cooney , C.A. , Ali , S.F. , and Poirier , L.A. 1997 . Measuring S-adenosylmethionine in whole blood, red blood cells and cultured cells using a fast preparation method and high-performance liquid chromatography . J. Chromatogr. B. 696 : 145 – 152 .
  • Young , S.N. and Shalchi , M. 2005 . The effect of methionine and S-adenosylmethionine on S-adenosylmethionine levels in the rat brain . J. Psychiatry Neurosci . 30 : 44 – 48 .

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.