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SEPARATIONS

Factors Affecting the Performance of Capillary Isoelectric Focusing in Dynamically Coated Capillaries Using Polyethylene Oxide Polymer

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Pages 1914-1931 | Received 06 Mar 2008, Accepted 03 Apr 2008, Published online: 16 Jun 2010

REFERENCES

  • Chang , P.-L. , Chiu , T.-C. , and Chang , H.-T. 2006 . Stacking, derivatization, and separation by capillary electrophoresis of amino acids from cerebrospinal fluids . Electrophoresis , 27 ( 10 ): 1922 – 1931 .
  • Dolnik , V. 2006 . Capillary electrophoresis of proteins 2003–2005 . Electrophoresis , 27 ( 1 ): 126 – 141 .
  • Hjerten , S. 1985 . High-performance electrophoresis. Elimination of electroendosmosis and solute adsorption . J. Chromatography , 347 ( 2 ): 191 – 198 .
  • Hjerten , S. and Zhu , M.D. 1985 . Adaptation of the equipment for high-performance electrophoresis to isoelectric focusing . J. Chromatography , 346 : 265 – 270 .
  • Horka , M. , Ruzicka , F. , Horky , J. , Hola , V. , and Slais , K. 2006 . Capillary isoelectric focusing of proteins and microorganisms in dynamically modified fused silica with UV detection . J. Chromatography, B: Analytical Technologies in the Biomedical and Life Sciences , 841 ( 1–2 ): 152 – 159 .
  • Horvath , J. and Dolnik , V. 2001 . Polymer wall coatings for capillary electrophoresis . Electrophoresis , 22 ( 4 ): 644 – 655 .
  • Huang , T.-L. and Richards , M. 1997 . Development of a high-performance capillary isoelectric focusing technique with application to studies of microheterogeneity in chicken conalbumin . J. Chromatography, A , 757 ( 1 + 2 ): 247 – 253 .
  • Huang , Y.-F. , Hsieh , M.-M. , Tseng , W.-L. , and Chang , H.-T. 2006 . On-line concentration of microheterogeneous proteins by capillary electrophoresis using SDS and PEO as additives . J. Proteome Res. , 5 ( 2 ): 429 – 436 .
  • Huang , Y.-F. , Huang , C.-C. , Hu , C.-C. , and Chang , H.-T. 2006 . Capillary electrophoresis-based separation techniques for the analysis of proteins . Electrophoresis , 27 ( 18 ): 3503 – 3522 .
  • Kilar , F. 2003 . Recent applications of capillary isoelectric focusing . Electrophoresis , 24 ( 22–23 ): 3908 – 3916 .
  • Kundu , S. and Fenters , C. 1995 . Isoelectric focusing of monoclonal antibodies by capillary electrophoresis . J. Capillary Electrophor. , 2 ( 6 ): 273 – 277 .
  • Kuo , C.-Y. , Chiou , S.-S. , and Wu , S.-M. 2006 . Solid-phase extraction and large-volume sample stacking with an electroosmotic flow pump in capillary electrophoresis for determination of methotrexate and its metabolites in human plasma . Electrophoresis , 27 ( 14 ): 2905 – 2909 .
  • Lee , H.G. 1997 . Rapid high-performance isoelectric focusing of monoclonal antibodies in uncoated fused-silica capillaries . J. Chromatography A , 790 ( 1 + 2 ): 215 – 223 .
  • Little , M.J. , Paquette , D.M. , and Roos , P.K. 2006 . Electrophoresis of pharmaceutical proteins: status quo . Electrophoresis , 27 ( 12 ): 2477 – 2485 .
  • Lupi , A. , Viglio , S. , Luisetti , M. , Gorrini , M. , Coni , P. , Faa , G. , et al. 2000 . Alpha(1)-antitrypsin in serum determined by capillary isoelectric focusing . Electrophoresis , 21 ( 15 ): 3318 – 3326 .
  • Mazzeo , J.R. and Krull , I.S. 1991 . Capillary isoelectric focusing of proteins in uncoated fused silica capillaries using polymeric additives . Anal. Chem. , 63 ( 24 ): 2852 – 2857 .
  • Mazzeo , J.R. , Martineau , J.A. , and Krull , I.S. 1992 . Performance of isoelectric focusing in uncoated and commercially available coated capillaries . Methods (San Diego, CA, United States) , 4 ( 3 ): 205 – 212 .
  • Miksik , I. , Sedlakova , P. , Mikulikova , K. , Eckhardt , A. , Cserhati , T. , and Horvath , T. 2006 . Matrices for capillary gel electrophoresis—a brief overview of uncommon gels . Biomedical Chromatography , 20 ( 6–7 ): 458 – 465 .
  • Mosher , R.A. and Thormann , W. 2002 . High-resolution computer simulation of the dynamics of isoelectric focusing using carrier ampholytes: The post-separation stabilizing phase revisited . Electrophoresis , 23 ( 12 ): 1803 – 1814 .
  • Patrick , J.S. and Lagu , A.L. 2001. Review applications of capillary electrophoresis to the analysis of biotechnology-derived therapeutic proteins. Electrophoresis , 22(19): 4179–4196.
  • Righetti , P.G. 2004 . Determination of the isoelectric point of proteins by capillary isoelectric focusing . J. Chromatography A , 1037 ( 1–2 ): 491 – 499 .
  • Righetti , P.G. , Gelfi , C. , and Conti , M. 1997 . Current trends in capillary isoelectric focusing of proteins . J. Chromatography B: Biomedical Sciences and Applications , 699 ( 1 + 2 ): 91 – 104 .
  • Rodriguez-Diaz , R. , Zhu , M. , and Wehr , T. 1997 . Strategies to improve performance of capillary isoelectric focusing . J. Chromatography A , 772 ( 1 + 2 ): 145 – 160 .
  • Shimura , K. 2002 . Recent advances in capillary isoelectric focusing: 1997–2001 . Electrophoresis , 23 ( 22–23 ): 3847 – 3857 .
  • Shimura , K. , Zhi , W. , Matsumoto , H. , and Kasai , K.-I. 2000 . Accuracy in the determination of isoelectric points of some proteins and a peptide by capillary isoelectric focusing: Utility of synthetic peptides as isoelectric point markers . Anal. Chem. , 72 ( 19 ): 4747 – 4757 .
  • Stoyanov , A.V. , Das , C. , Fredrickson , C.K. , and Fan , Z.H. 2005 . Conductivity properties of carrier ampholyte pH gradients in isoelectric focusing . Electrophoresis , 26 ( 2 ): 473 – 479 .
  • Strelec , I. , Pacakova , V. , Bosakova , Z. , Coufal , P. , Guryca , V. , and Stulik , K. 2002 . Modification of capillary electrophoresis capillaries by poly(hydroxyethyl methacrylate), poly(diethylene glycol monomethacrylate) and poly(triethylene glycol monomethacrylate) . Electrophoresis , 23 ( 4 ): 528 – 535 .
  • Tang , Q. and Lee , C.S. 1997 . Effects of electroosmotic flow on zone mobilization in capillary isoelectric focusing . J. Chromatography A , 781 ( 1 + 2 ): 113 – 118 .
  • Tauzin , B. 2006 . 418 Biotechnology Medicines in Testing Promise to Bolster the Arsenal Against Disease . (Report) Medicines in Development, Biotechnology: Pharmaceutical Research and Manufactures of America (www.phrma.org/files/Biotech%202006.pdf) .
  • Tran , N.T. , Taverna , M. , Miccoli , L. , and Angulo , J.F. 2005 . Poly(ethylene oxide) facilitates the characterization of an affinity between strongly basic proteins with DNA by affinity capillary electrophoresis . Electrophoresis , 26 ( 16 ): 3105 – 3112 .
  • Vayaboury , W. , Kirby , D. , Giani , O. , and Cottet , H. 2005 . Noncovalent coatings for the separation of synthetic polypeptides by nonaqueous capillary electrophoresis . Electrophoresis , 26 ( 11 ): 2187 – 2197 .
  • Wehr , T. 2005 . Capillary electrophoresis in the biopharmaceutical industry: Part II . LCGC North America , 23 ( 11 ): 1182,1186,1188,1190 .
  • Yeung , K.K.C. , Atwal , K.K. , and Zhang , H. ( 2003 ). Dynamic capillary coatings with zwitterionic surfactants for capillary isoelectric focusing . Analyst (Cambridge, United Kingdom) , 128 ( 6 ): 566 – 570 .
  • Zhang , C.-X. , Xiang , F. , Pasa-Tolic , L. , Anderson , G.A. , Veenstra , T.D. , and Smith , R.D. 2000 . Stepwise mobilization of focused proteins in capillary isoelectric focusing mass spectrometry . Anal. Chem. , 72 ( 7 ): 1462 – 1468 .

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