109
Views
3
CrossRef citations to date
0
Altmetric
ELECTROPHORESIS

Rapid and Sensitive Method to Detect Oxidized Forms of rhGCSF Using Agilent 2100 Bioanalyzer

, , , &
Pages 1070-1083 | Received 05 Dec 2008, Accepted 12 Mar 2008, Published online: 28 May 2009

REFERENCES

  • Brena , R.M. , Auer , H. , Kornacker , K. , Hackanson , B. , Raval , A. , Byrd , J.C. , and Plass , C. 2006 . Accurate quantification of DNA methylation using combined bisulfite restriction analysis coupled with the Agilent 2100 bioanalyzer platform . Nucleic Acids Res. , 34 : e17 .
  • Brot , N. and Weissbach , H. 1983 . Biochemistry and physiological role of methionine sulfoxide residues in proteins . Arch. Biochem. Biophys. , 223 : 271 – 281 .
  • DalleDonne , I. , Milzani , A. , and Colombo , R. 1999 . The tert-butyl hydroperoxide-induced oxidation of actin Cys-374 is coupled with structural changes in distant regions of the protein . Biochemistry , 38 : 12471 – 12480 .
  • Duenas , E.T. , Keck , R. , Vos , D. , Jones , A.S. , and Cleland , J.L. 2001 . Comparison between light induced and chemically induced oxidation of rhVEGF . Pharm. Res. , 18 : 1455 – 1460 .
  • Gao , J. , Yin , D.H. , Yao , Y. , Williams , T.D. , and Squier , T.C. 1998a . Progressive decline in the ability of calmodulin isolated from aged brain to activate the plasma membrane Ca-ATPase . Biochemistry , 37 : 9536 – 9548 .
  • Gao , J. , Yin , D.H. , Yao , Y. , Sun , H. , Qin , Z. , Schöneich , C. , Williams , T.D. , and Squier , T.C. 1998b . Loss of conformational stability in calmodulin upon methionine oxidation . Biophys. J. , 74 : 1115 – 1134 .
  • Gottwald , E. , Müller , O. , and Polten , A. 2001 . Semiquantitative reverse transcription–polymerase chain reaction with the Agilent 2100 bioanalyzer . Electrophoresis , 22 : 4016 – 4022 .
  • Halliwell , B. and Gutteridge , J.M.C. 1999 . Free Radicals in Biology and Medicine, , 3rd ed. New York : Oxford University Press .
  • Hoshi , T. and Heinemann , S.H. 2001 . Regulation of cell function by methionine oxidation and reduction . J. Physiol. , 531 : 1 – 11 .
  • Houghten , R.A. and Li , C.H. 1983 . Reduction of sulfoxides in peptides and proteins . Methods Enzymol. , 91 : 549 – 559 .
  • Keck , R.G. 1996 . The use of t-butyl hydroperoxide as a probe for methionine oxidation in proteins . Anal. Biochem. , 236 : 56 – 62 .
  • Kim , Y.H. , Berry , A.H. , Spencer , D.S. , and Stites , W.E. 2001. Comparing the effect on protein stability of methionine oxidation versus mutagenesis: Steps toward engineering oxidative resistance in proteins. Protein Eng. , 14: 343–347.
  • Levine , R.L. , Mosoni , L. , Berlett , B.S. , and Stadtman , E.R. 1996 . Methionine residues as endogenous antioxidants in proteins . Proc. Natl. Acad. Sci. USA , 93 : 15036 – 15040 .
  • Li , S. , Schöneich , C. , and Borchardt , R.T. 1995 . Chemical instability of protein pharmaceuticals: Mechanisms of oxidation and strategies for stabilization . Biotechnol. Bioeng. , 48 : 490 – 500 .
  • Lischwe , M.A. and Sung , M.T. 1977 . Use of chlorosuccinamide/urea for the selective cleavage of tryptophanyl peptide bonds in proteins . J. Biol. Chem. , 252 : 4976 – 4980 .
  • Liu , J.L. , Lu , K.V. , Eris , T. , Katta , V. , Westkott , K.R. , Narhi , L.O. , and Lu , H.S. 1998 . In vitro methionine oxidation of recombinant human leptin . Pharm. Res. , 15 : 632 – 640 .
  • Lu , H.S. , Fausset , P.R. , Narhi , L.O. , Horan , T. , Shinagawa , K. , Shimamoto , G. , and Boone , T.C. 1999 . Chemical modification and site-directed mutagenesis of methionine residues in recombinant human granulocyte colony-stimulating factor: Effect on stability and biological activity . Arch. Biochem. Biophys. , 362 : 1 – 11 .
  • Maas , C. , Hermeling , S. , Bouma , B. , Jiskoot , W. , and Gebbink , M.F. 2007 . A role for protein misfolding in immunogenicity of biopharmaceuticals . J. Biol. Chem. , 282 : 2229 – 2236 .
  • Mussa , N.A. , Cheung , K.Y. , Alam , I.M. , and Flatman , S. 2000 . The application of the 2100 bioanalyzer-protein chip in the biotechnology industry. Available at http://www.lonza.com/group/en/company/news/publications .
  • Nabuchi , Y. , Fujiwara , E. , Ueno , K. , Kuboniwa , H. , Asoh , Y. , and Ushio , H. 1995 . Oxidation of recombinant human parathyroid hormone: Effect of oxidized position on the biological activity . Pharmacol. Res. , 12 : 2049 – 2052 .
  • Pavlou , A.K. and Reichert , J.M. 2004 . Recombinant protein therapeutics: Success rates, market trends, and values to 2010 . Nat. Biotech. , 22 : 1513 – 1519 .
  • Reubsaet , J.L. , Beijnen , J.H. , Bult , A. , Hop , E. , Scholten , S.D. , Teeuwsen , J. , and Underberg , W.J. 1998 . Oxidation of recombinant methionyl human granulocyte colony stimulating factor . J. Pharm. Biomed. Anal. , 17 : 283 – 289 .
  • Shirafuji , N. , Asano , S. , Matsuda , S. , Watari , K. , Takaku , F. , and Nagata , S. 1989 . A new bioassay for human granulocyte colony-stimulating factor (hG-CSF) using murine myeloblastic NFS-60 cells as targets and estimation of its levels in sera from normal healthy persons and patients with infectious and hematological disorders . Exp. Hematol. , 17 : 116 – 119 .
  • Skufca , P. , Seibert , F. , and Grimm , R. 2008 . Stable aqueous G-CSF containing compositions. U.S. Patent Application 20080026046 .
  • Sodowich , B.I. , Fadl , I. , and Burns , C. 2007 . Method validation of in vitro RNA transcript analysis on the Agilent 2100 bioanalyzer . Electrophoresis , 28 : 2368 – 2378 .
  • Stadtman , E.R. 1992 . Protein oxidation and aging . Science. , 257 : 1220 – 1224 .
  • Tsuchiya , M. , Nomura , H. , Asano , S. , Kaziro , Y. , and Nagata , S. 1987. Characterization of recombinant human granulocyte-colony-stimulating factor produced in mouse cells. EMBO J. , 6: 611–616.
  • Underberg , W.J. , Hoitink , M.A. , Reubsaet , J.L. , and Waterval , J.C. 2000 . Separation and detection techniques for peptides and proteins in stability research and bioanalysis . J. Chromatogr. B , 742 : 401 – 409 .
  • Van Patten , S.M.V. , Hanson , E. , Bernasconi , R. , Zhang , K. , Manavalan , P. , Cole , E.S. , McPherson , J.M. , and Edmunds , T. 1999 . Oxidation of methionine residues in antithrombin: Effects on biological activity and heparin binding . J. Biol. Chem. , 274 : 10268 – 10276 .
  • Vanz , A.L.S. , Renard , G. , Palma , M.S. , Chies , J.M. , Dalmora , S.L. , Basso , L.A. , and Santos , D.S. 2008 . Human granulocyte colony stimulating factor (hG-CSF): Cloning, overexpression, purification, and characterization . Microb. Cell Fact. , 7 : 1 – 12 .
  • Volkin , D.B. , Mach , H. , and Middaugh , C.R. 1997 . Degradative covalent reactions important to protein stability . Mol. Biotechnol. , 8 : 105 – 122 .
  • Whitelegge , J.P. , Penn , B. , To , T. , Johnson , J. , Waring , A. , Sherman , M. , Stevens , R.L. , Fluharty , C.B. , Faull , K.F. , and Flauharty , A.L. 2000 . Methionine oxidation within the cerebroside–sulfate activator protein (CSAct or Saposin B) . Protein Sci. , 9 : 1618 – 1630 .
  • Yin , J. , Chu , J.W. , Ricci , M.S. , Brems , D.N. , Wang , D.I.C. , and Trout , B.L. 2005 . Effects of excipients on the hydrogen peroxide–induced oxidation of methionine residues in granulocyte colony-stimulating factor . Pharmacol. Res. , 22 : 141 – 147 .

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.