A Simple Simultaneous Colorimetric Determination of Primary and Secondary Amines with Fluorescamine

References

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• As shown in Table I, the absorption characteristics of chromophores vary, depending on the nature of the amine. Therefore, the amines used for the standardization should be identical, or structurally as close as possible, to those under investigation
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• Very weak basic amines such as p-nitroaniline are best determined in a non-aqueous solvent such as acetonitrile, using a tertiary amine as catalyst
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• Application of fluorescamine for colorimetric analysis of peptides and proteins (estimation of N-methyl substituted) is under investigation
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