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BIOANALYTICAL

Rapid and Efficient Proteolysis for Protein Analysis by an Aptamer-Based Immobilized Chymotrypsin Microreactor

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Pages 868-878 | Received 14 Aug 2012, Accepted 18 Sep 2012, Published online: 01 Mar 2013

REFERENCES

  • Bishop , G. R. , J. Ren , B. C. Polander , B. D. Jeanfreau , J. O. Trent , and J. B. Chaires . 2007 . Energetic basis of molecular recognition in a DNA aptamer . Biophys. Chem. 126 ( 1–3 ): 165 – 175 .
  • Chait , B. T. 2006 . Chemistry. Mass spectrometry: bottom-up or top-down? Science 314 ( 5796 ): 65 – 66 .
  • de-los-Santos-Álvarez , N. , M. A. J. Lobo-Castañón , A. J. Miranda-Ordieres , and P. Tuñón-Blanco . 2008 . Aptamers as recognition elements for label-free analytical devices . TrAC Trends in Anal. Chem. 27 ( 5 ): 437 – 446 .
  • Ellington , A. D. , and J. W. Szostak . 1990 . In vitro selection of RNA molecules that bind specific ligands . Nature 346 ( 6287 ): 818 – 822 .
  • Gubala , V. , L. F. Harris , A. J. Ricco , M. X. Tan , and D. E. Williams . 2012 . Point of care diagnostics: Status and future . Anal. Chem. 84 ( 2 ): 487 – 515 .
  • Iliuk , A. B. , L. Hu , and W. A. Tao . 2011 . Aptamer in bioanalytical applications . Anal. Chem. 83 ( 12 ): 4440 – 4452 .
  • Jiang , Y. K. , L. C. Sun , Q. F. Cai , G. M. Liu , A. Yoshida , K. Osatomi , and M. J. Cao . 2010 . Biochemical characterization of chymotrypsins from the hepatopancreas of Japanese sea bass (Lateolabraxjaponicus) . J. Agric. Food Chem. 58 ( 13 ): 8069 – 8076 .
  • Krenkova , J. , and F. Svec . 2009 . Less common applications of monoliths: IV. Recent developments in immobilized enzyme reactors for proteomics and biotechnology . J. Sep. Sci. 32 ( 5–6 ): 706 – 718 .
  • Liang , Y. , D. Tao , J. Ma , L. Sun , Z. Liang , L. Zhang , and Y. Zhang . 2011 . Hydrophilic monolith based immobilized enzyme reactors in capillary and on microchip for high-throughput proteomic analysis . J. Chromatogr. A 1218 ( 20 ): 2898 – 2905 .
  • Lin , C. H. , W. Wang , R. A. Jones , and D. J. Patel . 1998. Formation of an amino-acid-binding pocket through adaptive zippering-up of a large DNA hairpin loop. Chem. Biol. 5(10): 555–572.
  • Liu , G. , X. Mao , J. A. Phillips , H. Xu , W. Tan , and L. Zeng . 2009 . Aptamer-nanoparticle strip biosensor for sensitive detection of cancer cells . Anal. Chem. 81 ( 24 ): 10013 – 10018 .
  • Ma , J. , L. Zhang , Z. Liang , W. Zhang , and Y. Zhang . 2007 . Monolith-based immobilized enzyme reactors: Recent developments and applications for proteome analysis . J. Sep. Sci. 30 ( 17 ): 3050 – 3059 .
  • Ma , J. , L. Zhang , Z. Liang , W. Zhang , and Y. Zhang . 2009 . Recent advances in immobilized enzymatic reactors and their applications in proteome analysis . Anal. Chim. Acta 632 ( 1 ): 1 – 8 .
  • Mairal , T. , V. C. Ozalp , P. Lozano Sanchez , M. Mir , I. Katakis , and C. K. O'Sullivan . 2008 . Aptamers: Molecular tools for analytical applications . Anal. Bioanal. Chem. 390 ( 4 ): 989 – 1007 .
  • Massolini , G. , and E. Calleri . 2005 . Immobilized trypsin systems coupled on-line to separation methods: Recent developments and analytical applications . J. Sep. Sci. 28 ( 1 ): 7 – 21 .
  • Missailidis , S. , and A. Hardy . 2009 . Aptamers as inhibitors of target proteins . Expert Opin. Ther. Pat. 19 ( 8 ): 1073 – 1082 .
  • Nicoli , R. , S. Rudaz , C. Stella , and J. L. Veuthey . 2009 . Trypsin immobilization on an ethylenediamine-based monolithic minidisk for rapid on-line peptide mass fingerprinting studies . J. Chromatogr. A 1216 ( 13 ): 2695 – 2699 .
  • Nie , Y. L. , and W. H. Wang . 2009 . Immobilized enzyme reactor in on-line LC and its application in drug screening . Chromatographia 69 ( 0 ): 5 – 12 .
  • Nimjee , S. M. , C. P. Rusconi , and B. A. Sullenger . 2005 . Aptamers: An emerging class of therapeutics . Annu. Rev. Med. 56 : 555 – 583 .
  • Robertson , D. L. , and G. F. Joyce . 1990 . Selection in vitro of an RNA enzyme that specifically cleaves single-stranded DNA . Nature 344 ( 6265 ): 467 – 468 .
  • Shapiro , E. , and B. Gil . 2008 . Cell biology. RNA computing in a living cell . Science 322 ( 5900 ): 387 – 388 .
  • Smith , J. C. , J. P. Lambert , F. Elisma , and D. Figeys . 2007 . Proteomics in 2005/2006: Developments, applications and challenges . Anal. Chem. 79 ( 12 ): 4325 – 4343 .
  • Tuerk , C. , and L. Gold . 1990 . Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase . Science 249 ( 4968 ): 505 – 510 .
  • Tyers , M. , and M. Mann . 2003 . From genomics to proteomics . Nature 422 ( 6928 ): 193 – 197 .
  • Xiao , P. , X. Lv , and Y. Deng . 2012 . Immobilization of Chymotrypsin on Silica Beads Based on High Affinity and Specificity Aptamer and Its Applications . Anal. Lett. 45 ( 10 ): 1264 – 1273 .
  • Zor , T. , and Z. Selinger . 1996 . Linearization of the Bradford protein assay increases its sensitivity: Theoretical and experimental studies . Anal. Biochem. 236 ( 2 ): 302 – 308 .

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