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Original Articles

Preservation of Entamoeba Histolytica at −79°C. in the Presence of Glycerol

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Pages 240-246 | Received 11 May 1953, Published online: 22 Mar 2016

REFERENCES

  • Coggeshall, L. T. (1939). Preservation of viable malaria parasites in the frozen state. Proc. Soc. Exp. Biol., N.Y., 42, 499.
  • Dobell, C., and Laidlaw, P. P. (1926). On the cultivation of Entamoeba histolytica and some other entozoic amoebae. Parasitology, 18, 283.
  • Fulton, J. D., Joyner, L. P., King, H., Osbond, J. M., and Wright, J. (1950). Amoebicidal action and chemical constitution. Proc. Roy. Soc., B, 137, 339.
  • Laidlaw, P. P., Dobell, C., and Bishop, A. (1928). Further experiments on the action of emetine in cultures of Entamoeba histolytica. Parasitology, 20, 207.
  • Levaditi, J. C. (1952). Possibilité de conserver Tr. congolense et Pl. berghei à—7o°C. C. R. Soc. Biol., Paris, 146, 179.
  • Lovelock, J. E. (1953). The mechanism of the protective action of glycerol against haemolysis by freezing and thawing. Biochim. Biophys. Acta, 11, 28.
  • Manwell, R. D. (1943). The low-temperature freezing of malaria parasites. Amer. J. Trop. Med., 23, 123.
  • Manwell, R. D.and Edgett, R. (1943). The relative importance of certain factors in the low-temperature preservation of malaria parasites. Ibid., 23, 551.
  • Mollison, P. L., Sloviter, H. A., and Chaplin, H. (1952). Survival of transfused red cells previously stored for long periods in the frozen state. Lancet, 2, 501.
  • Parkes, A. S., and Smith, A. U. (1953). Regeneration of rat ovarian tissue grafted after exposure to low temperatures. Proc. Roy. Soc., B, 140, 455.
  • Polge, C. (1951). Functional survival of fowl spermatozoa after freezing at—79°C. Nature, Lond., 167, 949.
  • Polge, C.and Lovelock, J. E. (1952). Preservation of bull semen at—79°C. Vet. Rec., 64, 396.
  • Polge, C.and Lovelock, J. E.and Rowson, L. E. A. (1952). Fertilizing capacity of bull spermatozoa after freezing at—79°C. Nature, Lond., 169, 626.
  • Polge, C.and Lovelock, J. E.and Rowson, L. E. A.Smith, A. U., and Parkes, A. S. (1949). Revival of spermatozoa after vitrification and dehydration at low temperatures. Ibid., 164, 666.
  • Smith, A. U., and Polge, C. (1950). Survival of spermatozoa at low temperatures. Ibid., 166, 668.
  • Smith, A. U., and Polge, C.and Smiles, J. (1951). Microscopic observation of living cells during freezing and thawing. J. R. Micr. Soc., 71, 186.
  • Weinman, D., and McAllister, J. (1947). Prolonged storage of human pathogenic protozoa with conservation of virulence: observations on the storage of helminths and leptospiras. Amer. J. Hyg., 45, 102.
  • Wolfson, F. (1945). Effect of preservation by freezing upon the virulence of Plasmodium for ducks. Ibid., 42. 155

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