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Journal of Apicultural Research Volume 40, 2001 - Issue 2
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Original Articles

An improved method for the detection and presumptive identification of Paenibacillus larvae subsp. larvae spores in honey

Dulce Maria Tocchetto SchuchMinistério da Agricultura e do Abastecimento, Laboratório Regional de Apoio Animal, Estrada da Ponta Grossa 3036, Porto Alegre, RS, BrazilCorrespondence[email protected]
,
Robert Heaviside MaddenFood Science Department (Food Microbiology), Department of Agriculture and Rural Development, Newforge Lane, BelfastBT9 5PX, UK
&
Aroni SattlerUniversidade Federal do Rio Grande do Sul, Faculdade de Agronomia, Departamento de Fitosanidade, Setor de Entomologia, Avenida Bento Gonçalves 7712, Porto Alegre, RS, Brazil
Pages 59-64 | Received 15 Jun 2000, Accepted 09 May 2001, Published online: 24 Mar 2015

REFERENCES

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  • BAILEY, L; LEE, D C (1962) Bacillus larvae: its cultivation in vitro and its growth in vivo. Journal of General Microbiology 29: 711–717.
  • GORDON, R E; HAYNES, W C; PANG, C H (1973) The genus Bacillus. Agriculture Handbook No. 427. Agricultural Research Service, United States Department of Agriculture, USA; 283 pp.
  • HANSEN, H (1984) The incidence of the foulbrood bacterium Bacillus larvae in honeys retailed in Denmark. Danish Research Service for Plant and Soil Science, Report No. 1725. Administrative Centre State Bee Disease Committee; Lyngby, Denmark; pp 329–336.
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  • SATTLER, A (1993) Investigação da ocorrência de esporos de Bacillus larvae em mel no Rio Grande do Sul, Brasil, e subsçdios para a prevenção e controle da Cria Ptrida Americana. Dissertação de mestrado do Programa de Pós Graduação em Agronomia da Universidade Federal do Rio Grande do Sul. Porto Alegre, Outubro; 84 pp.
  • SATTLER, A; LELL C; BASSI, E A; MASSAO, H (1996) Diagnóstico patológico da apicultura no Estado do Paraná—CITPAR-SIDEE/FEPA (GTZ). Proceedings; XI Simpósio Estadual de Apicultura do Paraná e VIII Exposição de Equipamentos e Materiais Apícolas; Pato Branco, PR; pp 50–53.
  • SCHUCH, D M T (2000) Barreiras Sanitárias na Apicultura—Ações do Governo Brasileiro para o controle da entrada da cria ptrida americana. Proceedings 2nd Seminário Sul Brasiliero de Apicultores; Balnerio Pinhal/RS; pp 47–52.
  • SHIMANUKI, H; KNOX, D A (1988) Improved method for the detection of Bacillus larvae spores in honey. American Bee Journal 128(5): 353–354.
  • SHIMANUKI, H; KNOX, D A (1991) American Foulbrood. Diagnosis of honey bee diseases. Agriculture Handbook No. 690. Agricultural Research Service, US Department of Agriculture; pp 4–12.
  • SHIMANUKI, H; KNOX, D A; FURGALA, B; CARON, D M; WILLIAMS, J L (1992) Diseases and pests of honey bees. In Graham, J M (ed) The hive and the honey bee. Dadant & Sons Inc.; Hamilton, II, USA; pp 1083–1151.

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