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Mycologia Volume 86, 1994 - Issue 3
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New Techniques

The effect of pH on staining of oospores of Peronospora viciae with tetrazolium bromide

Dirk Jan van der GaagDepartment of Phytopathology, Wageningen Agricultural University, P.O. Box 8025, 6700 EEWageningen, The Netherlands
Pages 454-457 | Accepted 19 Jan 1994, Published online: 29 Aug 2018

LITERATURE CITED

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  • An, Z.-Q., and J. W. Hendrix. 1988. Determining viability of endogonaceous spores with a vital stain. Mycologia 80: 259–261.
  • Binder, W. D., G. M. Mitchell, and D. J. Ballantyne. 1974. Pollen viability testing storage and related physiology: review of the literature with emphasis on gymnosperm pollen. Canadian Forestry Service, Victoria, B.C., Canada. 37 pp.
  • Bowers, J. H., G. C. Papavizas, and S. A. Johston. 1990. Effect of soil temperature and soil-water matric potential on the survival of Phytophthora capsici in natural soil. Pl. Dis. 74: 771–777.
  • Cohen, S. D. 1984. Detection of mycelium and oospores of Phytophthora megasperma J. sp. glycinea by vital stain in soil. Mycologia 76: 34–39.
  • El-Hamalawi, Z. A., and D. C. Erwin. 1986. Physical, enzymic, and chemical factors affecting viability and germination of oospores of Phytophthora megasperma f. sp. medicaginis. Phytopathology 76: 503–507.
  • Hess, R., and A. G. E. Pearse. 1963. Pathways of reduced pyridine nucleotide oxidation in rat-brain homogenate demonstrated by a tetrazolium method. Biochim. Biophys. Acta 71: 285–294.
  • Jiang, J., and D. C. Erwin. 1990. Morphology, plasmolysis, and tetrazolium bromide stain as criteria for determining viability of Phytophthora oospores. Mycologia 82: 107–113.
  • Kopooshian, H. 1968. A handbook for the tetrazolium test. Magon: publication de L'Institute de recherches agronomiques Liban. Serie scientific, no. 18. Tel-Amara, Lebanese Republic. 47 pp.
  • Mackay, D. B. 1972. The measurement of viability. Pp. 172–208. In: Viability of seeds. Ed., E. H. Roberts. Chapman and Hall, London, United Kingdom.
  • Nelson, G. A., and O. A. Olsen. 1967. Staining reactions of resting sporangia of Synchytrium endobioticum with a tetrazolium compound. Phytopathology 57: 965–968.
  • Pathak, V. K., S. B. Mathur, and P. Neergaard. 1978. Detection of Peronospora manshurica (Naum) Syd. in seeds of soybean, Glycine max. Eppo Bull. 8: 21–28.
  • Shetty, H. S., P. Neergaard, and S.B. Mathur. 1977. Demonstration of seed transmission of downy mildew or green ear disease, Sclerospora graminicola, in pearl millet, Pennisetum typhoides. Proc. Natl. Acad. Sci. India B 1, 43: 201–206.
  • Sutherland, E. D., and S. D. Cohen. 1983. Evaluation of tetrazolium bromide as a vital stain for fungal oospores. Phytopathology 73: 1532–1535.

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