Advanced search
Publication Cover
Mycologia Volume 91, 1999 - Issue 1
Submit an article Journal homepage
77
Views
4
CrossRef citations to date
0
Altmetric
Techniques

An optimized method for the isolation of protoplasts from Penicillium simplicissimum to produce sealed plasma membrane vesicles

Martin GallmetzerInstitute of Microbiology, University of Innsbruck, Technikerstrasse 25, 6020Innsbruck, AustriaCorrespondence[email protected]
,
Wolfgang BurgstallerInstitute of Microbiology, University of Innsbruck, Technikerstrasse 25, 6020Innsbruck, Austria
&
Franz SchinnerInstitute of Microbiology, University of Innsbruck, Technikerstrasse 25, 6020Innsbruck, Austria
Pages 206-212 | Accepted 31 Jul 1998, Published online: 04 Jun 2019

LITERATURE CITED

  • Blowers DP, Boss WF, Trewavas AJ. 1988. Rapid changes in plasma membrane protein phosphorylation during initiation of cell wall digestion. Pl Physiol 86: 505–509.
  • Burgstaller W, Müller B, Schinner F. 1997. The in vivo effect of glucose and extracellular pH on the plasma membrane H+-ATPase of Penicillium simplicissimum. FEMS Microbiol Lett 147: 109–114.
  • Burgstaller W, Müller B, Schinner F., Zanella A, Schinner F. 1994. Buffer-stimulated citrate efflux in Penicillium simplicissimum: An alternative charge balancing ion flow in case of reduced proton backflow? Arch Microbiol 161: 75–81.
  • Butt TM, Hoch HC, Staples RC, St. Leger RJ. 1989. Use of fluorochromes in the study of fungal cytology and differentiation. Exp Mycol 13: 303–320.
  • Fawcett PA, Loder PB, Duncan MJ, Beesley TJ, Abraham EP. 1973. Formation and properties of protoplasts from antibiotic-producing strains of Penicillium chrysogenum and Cephalosporium acremonium. J Gen Microbiol 79: 293–309.
  • Hamlyn PF, Bradshaw RE, Mellon FM, Santiago CM, Wilson JM, Peberdy JF. 1981. Efficient protoplast isolation from fungi using commercial enzymes. Enzyme Microbiol Technol 3: 321–325.
  • Huang C-N, Cornejo MJ, Bush DS, Jones RL. 1986. Estimating viability of plant protoplasts using double and single staining. Protoplasma 135: 80–87.
  • Isaac S, Gokhale AV. 1982. Autolysis: a tool for protoplast production from Aspergillus nidulans. Trans Brit Mycol Soc 78: 389–394.
  • Isaac S, Gokhale AV., Wyatt AM. 1986. Selectivity to K+ and Na+ of protoplast fractions isolated from different regions of Aspergillus nidulans hyphae. J Gen Microbiol 132: 1173–1179.
  • Ishikawa F, Oishi K. 1985. Formation and regeneration of protoplasts from Conidiobolus lamprauges. J Gen Microbiol 131: 3311–3316.
  • Kaile A, Pitt D, Kuhn PJ. 1992. Calcium cytotoxicity, protoplast viability and the role of calcium in soft-rot of Brassica napus due to Botrytis cinerea Pers. Physiol Molec Pl Pathol 40: 49–62.
  • Menendez A, Larsson C, Ugalde UO. 1995. Purification of functionally sealed cytoplasmic side-out plasma membrane vesicles from Saccharomyces cerevisiae. Anal Biochem 230: 308–314.
  • Palmgren MG, Askerlund P, Fredrikson K, Widell S, Sommarin M, Larsson C. 1990. Sealed inside-out and right-side-out plasma membrane vesicles. Pl Physiol 92: 871–880.
  • Peberdy JF. 1991. Fungal protoplasts. In: Bennet JW, Lasure LL, eds. More gene manipulation in fungi. New York: Academic Press. p 307–318.
  • Peberdy JF., Buckley CE, Daltrey DC, Moore PM. 1976. Factors affecting protoplast release in some filamentous fungi. Trans Brit Mycol Soc 67: 23–26.
  • Petrov VV, Smirnova VV, Okorokov LA. 1992. Mercaptoethanol and dithiothreitol decrease the difference of electrochemical proton potentials across the yeast plasma and vacuolar membranes and activate their H+-ATPases. Yeast 8: 589–598.
  • Pitt D, Barnes JC, Ugalde UO. 1988. Differential uptake of calcium by strains of Penicillium notatum and relationship to calcium-induced conidiation. Trans Brit Mycol Soc 91: 489–499.
  • Roncal T, Ugalde UO, Barnes J, Pitt D. 1991. Production of protoplasts of Penicillium cyclopium with improved viability and functional properties. J Gen Microbiol 137: 1647–1651.
  • Sanders D. 1988. Fungi. In: Baker DA, Hall JL, eds. Solute transport in plant cells and tissues. Harlow, Essex: Longman Scientific & Technical. p 106–165.
  • Schinner F, Burgstaller W. 1989. Extraction of zinc from industrial waste by a Penicillium sp. Appl Environm Microbiol 55: 1153–1156.
  • Schnitzer M. 1987. Möglichkeiten der genetischen Rekombination bei Tolypocladium inflatum [PhD Thesis]. Innsbruck, Austria: Innsbruck University. 293 p.
  • Serrano R. 1988. Structure and function of proton trans-locating ATPase in plasma membranes of plants and fungi. Biochim Biophys Acta 947: 1–28.
  • Slayman CL, Kaminsky P, Stetson D. 1990. Structure and function of fungal plasma membrane ATPases. In: Kuhn PJ, Jung MJ, Goosey MW, Copping LG, eds. Biochemistry of cell walls and membranes in fungi. Berlin: Springer. p 299–314.
  • Ugalde UO. 1991. Preparation of highly purified plasma membrane vesicles of controlled orientation from Penicillium cyclopium. Mycol Res 95: 1303–1307.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Related research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.