Efficacy of propidium iodide and FUN-1 stains for assessing viability in basidiospores of Rhizopogon roseolus

LITERATURE CITED

• Barcellona ML, Favilla R, Berger JV, Avitabile M, Ragusa N, Masotti L. 1986. DNA–4′,6-diamidino-2-phenylindole interactions: a comparative study employing fluorescente and ultraviolet spectroscopy. Archives of Biochemistry and Biophysics 250:48–53.
   PubMed Web of Science ®Google Scholar
• Berg NO. 1951. A histological study of marked lipids. Acta pathologica et microbiologica Scandinavica, Supplementum 90:1–192.
   Google Scholar
• Bornman C. 1989. Protoplast in practice. Lund, Sweden: University of Lund.
   Google Scholar
• Bruns TD, Peay KG, Boynton PJ, Grubisha LC, Hynson NA, Nguyen NH, Rosenstock, NP. 2009. Inoculum potential of Rhizopogon spores increases with time over the first 4 yr of 99-yr spore burial experiment. New Phytologist 181:463–470.
   PubMed Web of Science ®Google Scholar
• Castellano MA, Molina R. 1989. The biological component: nursery pests and mycorrhizae. In: Landis TD, Tinus RW, McDonald SE, Barnett JP, eds. The container tree nursery manual. Washington, DC: United States Department of Agriculture Forest Service. p. 101–167.
   Google Scholar
• Colgan W, Claridge AW. 2002. Mycorrhizal effectiveness of Rhizopogon spores recovered from the faecal pellets of forest dwelling small mammals. Mycological Research 106:314–320.
   Web of Science ®Google Scholar
• Duckett JG, Read DJ. 1991. The use of the fluorescent dye, 3,3′-dihexyloxacarbocyanine iodide, for selective staining of ascomycete fungi associated with liverwort rhizoids and ericoid mycorrhizal roots. New Phytologist 118:259–272.
   PubMed Web of Science ®Google Scholar
• Esteban M, Paz MŠ, Grācia E. 2000. Spore germination and development of young mycelia in some Rhizopogon species. Acta Botanica Barcinonensia 46:31–46.
   Google Scholar
• Fries N. 1978. Basidiospore germination in some mycorrhiza-forming hymenomycetes. Transactions of the British Mycological Society 70:319–324.
   Web of Science ®Google Scholar
• Fries N. 1984. Spore germination in the higher Basidiomycetes. Proceedings of the Indian Academy of Sciences (Plant Sciences) 93:205–222.
   Google Scholar
• Furch B. 1981. Spore germination: heat mediated events. In: Turian G, Hohl. HR, eds. The fungal spore: morphogenetic controls. New York, New York: Academic Press, p. 413–433.
   Google Scholar
• Ivey MLL, Miller SA. 2014. Use of the vital stain FUN-1 indicates viability of Phytophthora capsici propagules and can be used to predict maximum zoospore production. Mycologia 106:362–367.
   PubMed Web of Science ®Google Scholar
• Kropp BR, Fortin JA. 1988. The incompatibility system and relative ectomycorrhizal performance of monokaryons and reconstituted dikaryons of Laccaria bicolor. Canadian Journal of Botany 66:289–294.
   Google Scholar
• Lamhamedi MS, Fortin JA, Kope HH, Kropp BR. 1990. Genetic variation in ectomycorrhiza formation by Pisolithus arhizus on Pinus pinaster and Pinus banksiana. New Phytologist 115:689–697.
   Web of Science ®Google Scholar
• Lehtinen J, Nuutila J, Lilius EM. 2004. Green fluorescent protein–propidium iodide (GFP‐PI) based assay for flow cytometric measurement of bacterial viability. Cytometry Part A 60:165–172.
   PubMed Web of Science ®Google Scholar
• Lilleskov EA, Bruns TD. 2005. Spore dispersal of a resupinate ectomycorrhizal fungus, Tomentella sublilacina, via soil food webs. Mycologia 97:762–769.
   PubMed Web of Science ®Google Scholar
• Martín MP, Ferran MJ. 1995. Examination of spores and young mycelia of Rhizopogon roseolus by scanning electron microscopy. Mycologist 9:121–123.
   Google Scholar
• Millard PJ, Roth BL, Truong Thi H-P, Yue ST, Haugland RP. 1997. Development on the FUN-1 family of fluorescent probes for vacuole labelling and viability testing yeast. Applied and Environmental Microbiology 63:2897–2905.
   PubMed Web of Science ®Google Scholar
• Miller SL, Torres P, McClean TM. 1993. Basidiospore viability and germination in ectomycorrhizal and saprotrophic basidiomycetes. Mycological Research 97:141–149.
   Web of Science ®Google Scholar
• Molina R, Trape JM. 1994. Biology of the ectomycorrhizal genus, Rhizopogon. I. Host associations, host-specificity and pure culture syntheses. New Phytologist 126:653–675.
   Web of Science ®Google Scholar
• Nakano S, Gao Q, Aimi T, Shimomura N. 2016. Rhizopogon roseolus basidiospore germination decreases as the fruiting bodies mature and the spore wall becomes more complex. Botany 94:311– 320.
   Google Scholar
• Nicoletti I, Migliorati G, Pagliacci MC, Grignani F, Riccardi C. 1991. A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry. Journal of Immunological Methods 139: 271–279.NiederpruemDJ, Dennen DW. 1966. Kinetics, nutriton and inhibitor properties of basiodiospore germination in Schizophyllum commune. Archiv für Mikrobiologie 54:91–105.
   Google Scholar
• Niederpruem DJ, Hafiz A, Henry L. 1965. Polyol metabolism in Basidiomycete Schyzophyllum commune. Journal of Bacteriology 89:954–959.
   PubMed Web of Science ®Google Scholar
• Saby S, Sibille I, Mathieu L, Paquin JL, Block JC. 1997. Influence of water chlorination on the counting of bacteria with DAPI (4′-6-diamidino-2-phenylindole). Applied and Environmental Microbiology 63:1564–1569.
   PubMed Web of Science ®Google Scholar
• Sánchez C, Moore D. 1999. Conventional histological stains selectively stain fruit body initials of basidiomycetes. Mycological Research 103:315–318.
   Web of Science ®Google Scholar
• Shimomura N, Aimi T, Matsumoto T, Maekawa N, Otani H. 2008. Ultrastructure of developing basidiospores in Rhizopogon roseolus (=R. rubescens). Mycoscience 49:35–41.
   Web of Science ®Google Scholar
• Smith AH, Zeller SM. 1966. A preliminary account of the North American species of Rhizopogon. Memoirs of the New York Botanical Garden 14:1–178.
   Google Scholar
• Stockwell MP, Clulow J, Mahony MJ. 2010. Efficacy of SYBR 14/propidium iodide viability stain for the amphibian chytrid fungus Batrachochytrium dendrobatidis. Diseases of Aquatic Organisms 88:177–181.
   PubMed Web of Science ®Google Scholar
• Sussman AS. 1966. Dormancy and spore germination. In: Ainsworth GC, Sussman AS, eds. The Fungi I. New York, New York: Academic Press. p. 733–761.
   Google Scholar
• Torres P, Honrubia M. 1994. Basidiospores viability in stored slurries. Mycological Research 98:527–530.
   Web of Science ®Google Scholar
• Vittal R, Haudenshield JS, Hartman GL. 2012. A multiplexed immunofluorescence method identifies Phakopsora pachyrhizi urediniospores and determines their viability. Phytopathology 102:1143–1152.
   PubMed Web of Science ®Google Scholar
• Wang X, Martindale JL, Holbrook, NJ. 2000. Requirement for ERK activation in cisplatin-induced apoptosis. Journal of Biological Chemistry 275:39435–39443.
   PubMed Web of Science ®Google Scholar
• Wagner F, Gay G, Debaud JC. 1988. Genetical variability of glutamate dehydrogenase activity in monokaryotic and dikaryotic mycelia of the ectomycorrhizal fungus Hebeloma cylindrosporum. Applied Microbiology and Biotechnology 28:566–571.
   Web of Science ®Google Scholar