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IMMUNOHISTOCHEMISTRY

Immunohistochemistry as a diagnostic aid in cervical pathology

Pages 97-111 | Received 28 Aug 2006, Accepted 29 Oct 2006, Published online: 06 Jul 2009

References

  • McCluggage W. G. Recent advances in immunohistochemistry in gynaecological pathology. Histopathology 2002; 40: 309–26
  • McCluggage W. G., Young R. H. Immunohistochemistry as a diagnostic aid in the evaluation of ovarian tumors. Semin Diagn Pathol 2005; 22: 3–32
  • McCluggage W. G. Endocervical glandular lesions. Controversial aspects and ancillary techniques. J Clin Pathol 2003; 56: 164–73
  • Marjoniemi V. ‐. M. Immunohistochemistry in gynecological pathology: a review. Pathology 2004; 36: 109–19
  • Nucci M. R., Castillon D. H., Bai H., et al. Biomarkers in diagnostic obstetric and gynecologic pathology: a review. Adv Anat Pathol 2003; 10: 55–68
  • Yaziji H., Gown A. M. Immunohistochemical analysis of gynecologic tumors. Int J Gynecol Pathol 2001; 20: 64–78
  • McCluggage W. G. Recent advances in immunohistochemistry in the diagnosis of ovarian neoplasms. J Clin Pathol 2000; 53: 327–34
  • McCluggage W. G. A critical appraisal of the value of immunohistochemistry in the diagnosis of uterine neoplasms. Adv Anat Pathol 2004; 11: 162–71
  • Sano T., Oyama T., Kashiwabara K., et al. Expression status of p16 prootein is associated with human papillomavirus oncogenic potential in cervical and genital lesions. Am J Pathol 1998; 153: 1741–8
  • Klaes R., Friedrich T., Spitkovsky D., et al. Overexpression of p16 INK4A as a specific marker for dysplastic and neoplastic epithelial cells of the cervix uteri. Int J Cancer 2001; 92: 276–84
  • Keating J. T., Cviko A., Reithdorf S., et al. Ki‐67, cyclin E and p16 are complimentary surrogate biomarkers for human papillomavirus‐related cervical neoplasia. Am J Surg Pathol 2001; 25: 884–91
  • Milde‐Langosch K., Reithdorf S., Kraws‐Poppinghaus A. Expression of cyclin‐dependent kinase inhibitors p16, p21 and p27 in HPV‐positive and HPV‐negative cervical adenocarcinomas. Virchows Arch 2001; 439: 55–61
  • O'Neill C. J., McCluggage W. G. p16 expression in the female genital tract and its value in diagnosis. Adv Anat Pathol 2006; 13: 8–15
  • Mittal K. Utility of MIB1 in evaluating cauterised cervical cone biopsy margins. Int J Gynecol Pathol 1999; 18: 211–4
  • McCluggage W. G., Tang L., Maxwell P., Bharucha H. Monoclonal antibody MIB1 in the assessment of cervical squamous intraepithelial lesions. Int J Gynecol Pathol 1996; 15: 131–6
  • Mittal K., Mesia A., Demopoulos R. L. MIB1 expression is useful in distinguishing dysplasia from atrophy in elderly women. Int J Gynecol Pathol 1999; 18: 122–4
  • Pirog E. C., Baergen R. N., Soslow R. A., et al. Diagnostic accuracy of cervical low‐grade squamous intraepithelial lesions is improved with MIB1 immunostaining. Am J Surg Pathol 2002; 26: 70–5
  • Kruse A. ‐J., Baak J. P., Helliesen T., et al. Evaluation of MIB‐1 positive cell clusters as a diagnostic marker for cervical intraepithelial neoplasia. Am J Surg Pathol 2002; 26: 1501–7
  • Harnden P., Kennedy W., Andrew A. C., Southgate J. Immunophenotype of transitional metaplasia of the uterine cervix. Int J Gynecol Pathol 1999; 18: 125–9
  • Klaes R., Friedrich T., Spitkovsky D., et al. Overexpression of p16 (INK4A) as a specific marker for dysplastic and neoplastic epithelial cells of the cervix uteri. Int J Cancer 2002; 92: 276–84
  • Klaes R., Benner A., Freidrich T., et al. p16 (INK4A) immunohistochemistry improves interobserver agreement in the diagnosis of cervical intraepithelial neoplasia. Am J Surg Pathol 2002; 26: 1387–99
  • Agoff S. N., Lin P., Morihara J., Mao C., Kiviat N. B., Koutsky L. A. p16 INK4A expression correlates with degree of cervical neoplasia: a comparison with Ki‐67 expression and detection of high‐risk HPV types. Mod Pathol 2003; 16: 665–73
  • Benevolo M., Mottolese M., Marandino F., et al. Immunohistochemical expression of p16 INK4A is predictive of HR‐HPV infection in cervical low‐grade lesions. Mod Pathol 2006; 19: 384–91
  • Tavassoli F. A., Devilee P., editors. World Health Organization Classification of Tumours. Pathology and Genetics. Tumours of the Breast and Female Genital Organs. IARC Press, Lyon 2003
  • McCluggage W. G., Maxwell P., McBride H. A., Hamilton P. W., Bharucha H. Monoclonal antibodies Ki‐67 and MIB1 in the distinction of tuboendometrial metaplasia from endocervical adenocarcinoma and adenocarcinoma in situ in formalin fixed material. Int J Gynecol Pathol 1995; 14: 209–16
  • Lee K. R., Sun D., Crum C. P. Endocervical intraepithelial glandular atypia (dysplasia): a histopathologic, human papillomavirus, and MIB‐1 analysis of 25 cases. Hum Pathol 2000; 31: 656–64
  • Pirog E. C., Isacson C., Szabolcs M. J., et al. Proliferative activity of benign and neoplastic endocervical epithelium and correlation with HPV DNA detection. Int J Gynecol Pathol 2002; 21: 22–6
  • Cameron R. I., Maxwell P., Jenkins D., McCluggage W. G. Immunohistochemical staining with MIB‐1, bcl2 and p16 assists in the distinction of cervical glandular intraepithelial neoplasia from tubo‐ endometrial metaplasia, endometriosis and microglandular hyperplasia. Histopathology 2002; 41: 313–21
  • McCluggage W. G., Maxwell P. Bcl‐2 and p21 staining of cervical tuboendometrial metaplasia. Histopathology 2002; 40: 107
  • Negri G., Egarter‐Vigi E., Kasal A., et al. p16 (INK4a) is a useful marker for the diagnosis of adenocarcinoma of the cervix uteri and its precursors. Am J Surg Pathol 2003; 27: 187–93
  • Riethdorf L., Riethdorf S., Lee K. R., Cviko A., Loning T., Crum C. P. Human papillomaviruses, expression of p16 INK4A, and early endocervical glandular neoplasia. Hum Pathol 2002; 33: 899–904
  • Ishikawa M., Fujii T., Nasumoto N., et al. Correlation of p16 INK4A overexpression with human papillomavirus infection in cervical adenocarcinomas. Int J Gynecol Pathol 2003; 22: 378–85
  • Marques T., Andrade L. A., Vassalo J. Endocervical tubal metaplasia and adenocarcinoma in situ: role of immunohistochemistry for carcinoembryonic antigen and vimentin in differential diagnosis. Histopathology 1996; 28: 549–50
  • Cina S. J., Richardson M. S., Austin R. M., Kurman R. J. Immunohistochemical staining for Ki‐67 antigen, carcinoembryonic antigen, and p53 in the differential diagnosis of glandular lesions of the cervix. Mod Pathol 1997; 10: 176–80
  • Wells M., Brown L. J. R. Symposium Part IV: Investigative approaches to endocervical pathology. Int J Gynecol Pathol 2002; 21: 360–7
  • Reithdorf L., O'Connell J. T., Riethdorf S., et al. Differential expression of MUC2 and MUC5AC in benign and malignant glandular lesions of the cervix. Virchows Arch 2000; 437: 365–71
  • Griffin N. R., Wells M. Semi‐quantitative immunohistochemical studies of blood group antigen A, B, H, Lea, Leb structures and Ii backbone chains in the normal human cervix and in cervical adenocarcinoma. Histochem J 1993; 25: 228–41
  • Griffen N. R., Wells M. Characterization of complex carbohydrates in cervical glandular intraepithelial neoplasia and invasive adenocarcinoma. Int J Gynecol Pathol 1994; 13: 319–29
  • Ibrahim E., Blackett A. D., Tidy J. A., Wells M. CD44 is a marker of endocervical neoplasia. Int J Gynecol Pathol 1999; 18: 101–8
  • Negri G., Romano F., Vittadello F., et al. Laminin‐5 γ2 chain immunohistochemistry facilitates the assessment of invasiveness and improves the diagnostic reproducibility of glandular lesions of the cervix uteri. Hum Pathol 2006; 37: 704–10
  • Stoltzfus P., Salo S., Eriksson E., et al. Laminin‐5 gamma2 chain expression facilitates detection of invasive squamous cell carcinoma of the uterine cervix. Int J Gynecol Pathol 2004; 23: 215–22
  • Park J. J., Sun D., Quade B. J., et al. Stratified mucin‐producing intraepithelial lesions of the cervix: adenosquamous or columnar cell neplasia?. Am J Surg Pathol 2000; 24: 1414–9
  • McCluggage W. G., Oliva E., Herrington C. S., McBride H., Young R. H. CD10 and calretinin staining of endocervical glandular lesions, endocervical stroma and endometrioid adenocarcinoma of the uterine corpus: CD10 positivity is characteristic of, but not specific for, mesonephric lesions and is not specific for endometrioid stroma. Histopathology 2003; 43: 144–50
  • Ordi J., Nogales F. F., Palacia A., et al. Mesonephric adenocarcinoma of the uterine corpus: CD10 expression as evidence of mesonephric differentiation. Am J Surg Pathol 2001; 25: 1540–5
  • Ordi J., Romagosa C., Tavassoli F. A., et al. CD10 expression in epithelial tissues and tumors of the gynecologic tract; a useful marker in the diagnosis of mesonephric, trophoblastic and clear cell tumors. Am J Surg Pathol 2003; 27: 178–86
  • McCluggage W. G., Sumathi V. P. Maxwell P CD10 is a sensitive and diagnostically useful marker of normal endometrial stroma and of endometrial stromal neoplasms. Histopathology 2001; 39: 273–8
  • Silver S. A., Devouassoux‐Shisheboran M., Mezetti T. P., Tavassoli F. A. Mesonephric adenocarcinomas of the uterine cervix: a study of 11 cases with immunohistochemical findings. Am J Surg Pathol 2001; 25: 379–87
  • Truskinovsky A. M., Stelow E. B., Jessurun J., et al. Staining for p16 of cervical mesonephric remnants and hyperplasia: a potential diagnostic pitfall. Mod Pathol 2006; 19: 925A
  • Utsugi K., Hira Y., Takeshima N., Akiyama F., Sakurai S., Hasumi K. Utility of the monoclonal antibody HIK1083 in the diagnosis of adenoma malignum of the uterine cervix. Gynecol Oncol 1999; 75: 345–8
  • Mikami Y., Kiyokawa T., Hata S., et al. Gastrointestinal immunophenotype in adenocarcinomas of the uterine cervix and related glandular lesions: a possible link between lobular endocervical glandular hyperplasia/pyloric gland metaplasia and adenoma malignum. Mod Pathol 2004; 17: 962–72
  • Ichimura T., Koizumi T., Tateiwa H., et al. Immunohistochemical expression of gastric mucin and p53 in minimal deviation adenocarcinoma of the uterine cervix. Int J Gynecol Pathol 2001; 20: 220–6
  • Steeper T. A., Wick M. E. Minimal deviation adenocarcinoma of the uterine cervix (‘adenoma malignum’). An immunohistochemical comparision with microglandular hyperplasia and conventional endocervical adenocarcinoma. Cancer 1986; 58: 1131–8
  • Gilks C. B., Young R. H., Aguirre P., et al. Adenoma malignum (minimal deviation adenocarcinoma) of the uterine cervix. A clinicopathological and immunohistochemical analysis of 26 cases. Am J Surg Pathol 1989; 13: 717–29
  • Mikami Y., Kiyokawa T., Hata S., et al. Gastrointestinal immunophenoctype in adenocarcinomas of the uterine cervix and related glandular lesions: a possible link between lobular endocervical glandular hyperplasia/pyloric gland metaplasia and ‘adenoma malignum’. Mod Pathol 2004; 17: 967–72
  • Mikami Y., Hata S., Melamed J., Fujiwara K., Manabe T. Lobular endocervical glandular hyperplasia is a metaplastic process with a pyloric gland phenotype. Histopathology 2001; 39: 364–72
  • Kondo T., Hashi A., Murata S., et al. Endocervical adenocarcinomas associated with lobular endocervical glandular hyperplasia: a report of four cases with histochemical and immunohistochemical analysis. Mod Pathol 2005; 15: 1199–210
  • Hashi A., Xu‐JY, Kondo T., et al. p16INK4A overexpression independent of human papillomavirus infection in lobular endocervical glandular hyperplasia. Int J Gynecol Pathol 2006; 25: 187–94
  • Mikami Y., Kiyokawa T., Moriya T., et al. Immunophenotypic alteration of the stromal component in minimal deviation adenocarcinoma (‘adenoma malignum’) and endocervical glandular hyperplasia: a study using oestrogen receptor and alpha‐smooth muscle actin double immunostaining. Histopathology 2005; 46: 130–6
  • Hayashi I., Tsuda H., Shimoda T. Reappraisal of orthodox histochemistry for the diagnosis of minimal deviation adenocarcinoma of the cervix. Am J Surg Pathol 2000; 24: 559–62
  • Nucci M. R., Ferry J. A., Young R. H. Ectopic prostatic tissue in the uterine cervix: a report of four cases and review of ectopic prostatic tissue. Am J Surg Pathol 2000; 24: 1224–30
  • McCluggage W. G., Ganesan R., Hirschowitz L., et al. Ectopic prostatic tissue in the uterine cervix and vagina: report of a series with a detailed immunohistochemical analysis. Am J Surg Pathol 2006; 30: 209–15
  • Staebler A., Sherman M. E., Zaino R. J., et al. Hormone receptor immunohistochemistry and human papillomavirus in situ hybridisation are useful for distinguishing endocervical and endometrial adenocarcinomas. Am J Surg Pathol 2002; 26: 998–1006
  • Castrillon D. H., Lee K. R., Nucci M. R. Distinction between endometrial and endocervical adenocarcinoma: an immunohistochemical study. Int J Gynecol Pathol 2002; 21: 4–10
  • McCluggage W. G., Sumathi V. P., McBride H. A., et al. A panel of immunohistochemical stains, including carcinoembryonic antigen, vimentin and estrogen receptor aids the distinction between primary endometrial and endocervical adenocarcinomas. Int J Gynecol Pathol 2002; 21: 11–5
  • Zaino R. J. The fruits of our labours: distinguishing endometrial from endocervical adenocarcinoma. Int J Gynecol Pathol 2002; 21: 1–3
  • McCluggage W. G., Jenkins D. Immunohistochemical staining with p16 may assist in the distinction between endometrial and endocervical adenocarcinoma. Int J Gynecol Pathol 2003; 22: 231–5
  • Ansari‐Lari M. A., Staebler A., Zaino R. J., Shah K. V., Ronnett B. M. Distinction of endocervical and endometrial adenocarcinomas: immunohistochemical p16 expression correlated with human papillomavirus (HPV) DNA detection. Am J Surg Pathol 2004; 28: 160–7
  • Armes J. E., Lourie R., de Silva M., et al. Abnormalities of the RB1 pathway in ovarian serous papillary carcinoma as determined by overexpression of the p16 (INK4A) protein. Int J Gynecol Pathol 2005; 24: 363–8
  • O'Neill C. J., McBride H. A., Connolly L. E., Deavers M. T., Malpica A., McCluggage W. G. High grade ovarian serous carcinoma exhibits significantly higher p16 expression than low grade serous carcinoma and serous borderline tumour. Histopathology, (in press)
  • Tambouret R., Clement P. B., Young R. H. Endometrial endometrioid adenocarcinoma with a deceptive pattern of spread to the uterine cervix: a manifestation of stage IIb endometrial carcinoma liable to be misinterpreted as an independent carcinoma or a benign lesion. Am J Surg Pathol 2003; 27: 1080–8
  • Kamoi S., Al Juboury M. L., Akin M. R., et al. Immunohistochemical staining in the distinction between endometrial and endocervical adenocarcinomas: another viewpoint. Int J Gynecol Pathol 2002; 21: 217–23
  • Elishaev E., Gilks C. B., Miller D., et al. Synchronous and metachronous endocervical and ovarian neoplasms: evidence supporting interpretation of the ovarian neoplasms as metastatic endocervical adenocarcinomas simulating primary ovarian surface epithelial neoplasms. Am J Surg Pathol 2005; 29: 281–94
  • Antilla M., Syrjanen S., Ji H., et al. Failure to demonstrate human papilloma virus DNA in epithelial ovarian cancer by general primer PCR. Gynecol Oncol 1999; 72: 337–41
  • Vang R., Gown A. M., Barry T. S., et al. Ovarian atypical proliferative (borderline) mucinous tumors: gastrointestinal and seromucinous (endocervical‐like) types are immunophenotypically distinctive. Int J Gynecol Pathol 2006; 25: 83–9
  • Gilks C. B., Young R. H., Gersell D. J., Clement P. B. Large cell neuroendocrine carcinoma of the uterine cervix: a clinicopathologic study of 12 cases. Am J Surg Pathol 1997; 21: 905–14
  • Albores‐Saavedra J., Latif S., Carrick K. S., et al. CD56 immunoreactivity in small cell carcinoma of the uterine cervix. Int J Gynecol Pathol 2005; 24: 113–7
  • Wang T. Y., Chen B. F., Yang Y. C., et al. Histologic and immunophenotypic classification of cervical carcinomas by expression of the p53 homologue p63: a study of 250 cases. Hum Pathol 2001; 32: 479–86
  • Horn L. ‐C., Lindner K., Szepankiewicz G. T. A., et al. p16, p14, p53, and cyclin D1 expression and HPV analysis in small cell carcinomas of the uterine cervix. Int J Gynecol Pathol 2006; 25: 182–6
  • Wang H. L., Lu D. Detection of human papillomavirus DNA and expression of p16, Rb, and p53 proteins in small cell carcinomas of the uterine cervix. Am J Surg Pathol 2004; 28: 901–8
  • Masumoto N., Fujii T., Ishikawa M., et al. p16INK4A overexpression and human papillomavirus infection in small cell carcinoma of the uterine cervix. Hum Pathol 2003; 34: 778–83
  • McCluggage W. G., Sargent A., Bailey A., et al. Large cell neuroendocrine carcinoma of the uterine cervix exhibiting TTF1 immunoreactivity. Histopathology, (in press)
  • Agoff S. N., Lamp T. W., Philip A. T., et al. Thyroid transcription factor‐1 is expressed in extrapulmonary small cell carcinomas but not in other extrapulmonary neuroendocrine tumors. Mod Pathol 2000; 13: 238–42
  • Jones T. D., Kernek K. M., Yang X. J., et al. Thyroid transcription factor 1 expression in small cell carcinoma of the urinary bladder: an immunohistochemical profile of 44 cases. Hum Pathol 2005; 36: 718–23
  • Chavez‐Blanco A., Taja‐Chayeb L., Cetina L., et al. Neuroendocrine marker expression in cervical carcinomas of non‐small cell type. Int J Gynecol Pathol 2002; 21: 368–74
  • Cennachi G., Pasquinelli G., Montanara L., et al. Primary endocervical extraosseous Ewing's sarcoma/PNET. Int J Gynecol Pathol 1998; 17: 83–8
  • Folpe A. L., Goldblum J. R., Rubin B. P., et al. Morphologic and immunophenotypic diversity in Ewing family tumors: a study of 66 genetically confirmed cases. Am J Surg Pathol 2005; 29: 1025–33
  • Jacques S. M., Qureshi F., Lawrence W. D. Surface epithelial changes in endometrial adenocarcinomas: diagnostic pitfalls in curettage specimens. Int J Gynecol Pathol 1995; 14: 191–7
  • Qiu W., Mittal K. Comparison of morphologic and immunohistochemical features of cervical microglandular hyperplasia with low‐grade mucinous adenocarcinoma of the endometrium. Int J Gynecol Pathol 2003; 22: 261–5
  • Plaza J. A., Ramirez N. C., Nuovo G. J. Utility of HPV analysis for evaluation of possible metastatic disease in women with cervical cancer. Int J Gynecol Pathol 2004; 23: 7–12
  • Zhang H., Liu J., Cagle P. T., et al. Distinction of pulmonary small cell carcinoma from poorly differentiated squamous cell carcinoma: an immunohistochemical approach. Mod Pathol 2006; 18: 111–8
  • Nielsen G. P., Oliva E., Young R. H., et al. Alveolar soft‐part sarcoma of the female genital tract: a report of nine cases and review of the literature. Int J Gynecol Pathol 1995; 14: 283–92
  • Roma A. A., Yang B., Senior M. E., Goldblum J. R. TFE3 immunoreactivity in alveolar soft part sarcoma of the uterine cervix: case report. Int J Gynecol Pathol 2005; 24: 131–5
  • Laskin W. B., Fetsch J. F., Tavassoli F. A. Superficial cervicovaginal myofibroblastoma: fourteen cases of a distinctive mesenchymal tumor arising from the specialized subepithelial stroma of the lower female genital tract. Hum Pathol 2001; 32: 715–25
  • Ganesan R., McCluggage W. G., Hirschowitz L., Rollason T. P. Superficial myofibroblastoma of the lower female genital tract: report of a series including tumours with a vulval location. Histopathology 2005; 46: 137–43
  • Kabbani W., Deavers M. T., Malpica A., et al. Uterine tumor resembling ovarian sex‐cord tumor: report of a case mimicking cervical adenocarcinoma. Int J Gynecol Pathol 2003; 22: 297–302
  • Irving J. A., Carinelli S., Prat J. Uterine tumors resembling ovarian sex cord tumors are polyphenotypic neoplasms with true sex cord differentiation. Mod Pathol 2006; 19: 17–24
  • McCluggage W. G. Uterine tumours resembling ovarian sex cord tumours: immunohistochemical evidence for true sex cord differentiation. Histopathology 1999; 34: 375–6
  • Shih I. M., Kurman R. J. Immunohistochemical localization of inhibin‐alpha in the placenta and gestational trophoblastic lesions. Int J Gynecol Pathol 1999; 18: 144–50
  • Mao T. ‐L., Seidman J., Kurman R. J., et al. Cyclin E and p16 immunoreactivity in epithelioid trophoblastic tumor – an aid in differential diagnosis. Am J Surg Pathol 2006; 30: 1105–10
  • Shih I. M., Kurman R. J. p63 expression is useful in the distinction of epithelioid trophoblastic and placental site trophoblastic tumors by profiling trophoblastic subpopulations. Am J Surg Pathol 2004; 28: 1177–83
  • Lagoo A. S., Robboy S. J. Lymphoma of the female genital tract: current status. Int J Gynecol Pathol 2006; 25: 1–21
  • Vang R., Medeiros L. J., Ha C. S., et al. Non‐Hodgkin's lymphomas involving the uterus: a clinicopathologic analysis of 26 cases. Mod Pathol 2000; 13: 19–28
  • Young R. H., Harris N. L., Scully R. E. Lymphoma‐like lesions of the lower female genital tract: a report of 16 cases. Int J Gynecol Pathol 1985; 4: 289–99
  • McGalie C. E., McBride H. A., McCluggage W. G. Cytomegalovirus infection of the cervix: morphological observations in five cases of a possibly under‐recognised condition. J Clin Pathol 2004; 57: 691–4

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