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Original Articles

Large Scale Preparation of Myelin Basic Protein from Central Nervous Tissue of Several Mammalian Species

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Pages 139-165 | Published online: 06 Dec 2006

References

  • Kies , M. W. , Thompson , E. B. and Alvord , E. C. Jr. 1964 . Sixth International Congress of Biochemistry . 1964 . Vol. 32 , pp. 656 IUB . VIII-53, Abstracts
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  • Volumes of solvents used in the preparation of myelin basic protein always refer to ml/g of frozen tissue used as starting material. Where two values are given the larger refers to the volume used in making basic protein preparations from spinal cord
  • Some lots of CM-52 have a relatively large amount of fines which interfere with rapid filtration of the alkaline CM-52 suspensions; although this appears not to affect the recovery of the purified myelin basic protein, one may wish to reject the lots of CM-52 which cause too much delay in this step of the procedure
  • Lowry , O. H. , Rosebrough , N. J. , Farr , A. L. and Randall , R. J. 1951 . J. Biol. Chem. , 193 : 265
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  • Deibler , G. E. , Martenson , R. E. and Kies , M. W. manuscript in preparation
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  • Martenson , R. E. , Deibler , G. E. , Kies , M. W. and Alvord , E. C. Jr. manuscript in preparation
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  • Bump , E. and Kies , M. W. unpublished observations

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