Isolation of Active cAMP Dependent Protein Kinases from Calf Ovaries: Gel Electrcphoresis vs. Gel Electrofocusting

References

• Protein kinase A, B and C and CAMP Binding Proteins A, B and C are capitalized in order to emphasize that the species previously identified by PAGE, and specifically Fig. 8 and Table 2 of ref., are referred to. In contrast to the previous study, we have however replaced kinase nad Binding Protein dasignations I to V by letters A to E, to emphasize their distinction from protein kinase designation I and II of Corbin and Keely 3
   Google Scholar
• Salokangas , A. , Talmadge , K. , Bechtel , E. , Eppenberger , U. and Chrambach , A. 1977 . Eur. J. Biochem. , 73 : 401 – 409 .
   Google Scholar
• Corbin , J. D. and Keely , S. L. 1977 . J. Biol. Chem. , 252 : 910 – 918 .
   PubMed Web of Science ®Google Scholar
• Deavo , J. A. , Bechtel , P. J. and Krebs , E. J. 1974 . Methods of Enzymology , Edited by: Colowick , S. P. and Kaplan , N. D. Vol. 38 , 299 – 308 . New York : Academic Press .
   Google Scholar
• Rubin , C. S. , Ehrlichman , J. and Posen , O. M. 1977 . J. Biol. Chem. , 244 : 36 – 44 .
   Google Scholar
• Tao , M. and Hackett , P. 1973 . J. Biol. Chem. , 248 : 5324 – 5332 .
   Google Scholar
• Viyamoto , E. , Petzold , G. L. , Kuo , J. F. and Greengard , J. F. 1973 . J. Biol. Chem. , 248 : 179 – 189 .
   Google Scholar
• Jungmann , R. A. , Hiestand , P. and Schweppe , J. S. 1974 . J. Biol. Chem. , 249 : 5444 – 5451 .
   PubMed Web of Science ®Google Scholar
• Taylor , S. , Lee , C.-Y. , Swain , L. and Stafford , P. H. 1976 . Anal. Biochem. , 76 : 45 – 52 .
   PubMedGoogle Scholar
• Huang , L. C. and Huang , C. 1975 . Biochemistry , 14 : 18 – 24 .
   PubMedGoogle Scholar
• Kaulen , H. D. and Cross , R. 1974 . Z. Physiol Chem. , 355 : 471 – 480 .
   Google Scholar
• Vesterova , M. V. , Sashchenco , L. P. , Vasiliev , V. Y. and Severin , E. S. 1975 . Biochim. Blophys. Acta , 377 : 271 – 281 .
   Web of Science ®Google Scholar
• Teririck , J. t. and Smith , A. J. 1968 . Arch. Biochera. Biophys. , 126 : 155 – 164 .
   Google Scholar
• Rodbard , D. and Chrambach , A. 1971 . Anal, Biochem. , 40 : 95 – 134 .
   PubMed Web of Science ®Google Scholar
• Ben-David , v. , Rodbard , D. , Bates , R. W. , Bridson , W. E. and Chrambach , A. 1973 . J. Clin. Endocr. vetab. , 36 : 951 – 964 .
   Google Scholar
• Talmadge , K. W. , Bechtel , E. , Salokangas , A. , Huber , P. , Jungmann , R. A. and Epoenberger , U. 1975 . Eur. J. Biochem. , 60 : 621 – 632 .
   Google Scholar
• For definitions of the total gel concentration, %T, the percent cross linking, %C, of the gel, the crosslinkers Bis and DATD, see refs. 1% and 18 and the table of abbreviations
   Google Scholar
• Chrambach , A. , Jovin , T. M. , Svendsen , P. J. and Rodbatrd , P. 1976 . Methods of Protein Separation , Edited by: Catsimponlas , N. Vol. 2 , 27 – 144 . New York, NY : Plenum Press .
   Google Scholar
• Bradford , M. 1976 . Anal. Biochem. , 72 : 248 – 252 .
   PubMed Web of Science ®Google Scholar
• Lowry , C. H. , Rosebrough , N. J. , Farr , A. L. and Randall , R. J. 1951 . J. Biol. Chem. , 193 : 265 – 275 .
   PubMed Web of Science ®Google Scholar
• Jovin , T. M. , Dante , M. L. and Chrambach , A. Multiphasic Puffer Systems Output , Springfield, VA : National Technical Information Service . 21251, 1970, Public Board Numbers 259311 and 196090
   Google Scholar
• Programs of D. Rodhard are available from Piomedical Computing Technology Information Certer, Room 1302, Vanderbil Medical Center, Nashville, Tennessee 37232, Program Identification Number: MED-34 PAGE-PACK
   Google Scholar
• Chrambach , A. 1980 . Molecular and Cellular Biochemistry , 29 : 23 – 46 .
   PubMedGoogle Scholar
• Baumann , G. and Chrambach , A. 1975 . Progress in Isoelectric Focusing and Isotacho Phoresis , Edited by: Righetti , P. G. 13 – 23 . Amsterdam, , The Netherlands : Elsevien, Excerpta Medica, North Holland Associated Scientific Publ. .
   Google Scholar
• Vesterbert , O. 1971 . Biochim. Biophys. Acta , 243 : 345 – 360 .
   Google Scholar
• Nguyen , N. Y. and Chrambach , A. 1976 . Anal. Blochem. , 74 : 145 – 153 .
   PubMed Web of Science ®Google Scholar
• Chrambach , A. and Nguyen , N. Y. 1977 . Electrorfocusing and Isotachophoresis , Edited by: Radola , B. J. and Graesslin , D. 51 – 58 . Berlin-New York : Walter de Gruyter .
   Google Scholar
• Nguyen , N. Y. and Chrambach , A. 1980 . Electrophoresis , 1 : 14 – 22 .
   Google Scholar
• Bjerten , S. 1976 . Methods of Protein Separation , Edited by: Catsimpoolas , V. Vol. 2 , 233 – 244 . New York, NY : Plenum Press .
   Google Scholar
• Hjelneland , L. M. and Chrombach , N. 1981 . Electrophoresis , 2 : 1 – 15 .
   Google Scholar
• Jackin , B. A. and Brown , E. K. 1980 . Electrophoresis , 1 : 107 – 112 .
   Google Scholar
• Gianazza , E. , Astorri , C. and Pighetti , P. C. 1979 . J. chromatogr. , 171 : 161 – 169 .
   Google Scholar
• Chrambach , A. , Hjelneland , L. M. , Nguyer , N. Y. and An der Lan , B. 1980 . Electrophoresis 79 , Edited by: Badola , B. J. 3 – 22 . Berlin New-York, NY : Balter de Gruyter .
   Google Scholar
• Nguyen , N. Y. and Chrambach , A. 1979 . J. Biochem, Biophys. Methods , 1 : 171 – 187 .
   Google Scholar
• Nauyen , N. Y. , Baumann , G. , Arhegast , D. E. , Grindeland , P. E. and Chrambach , A. 1980 . Preparative Biochem. , 11 : 139 – 157 .
   Google Scholar
• Nguyen , N. Y. , DiFonzo , J. and Charmbach , A. 1980 . Anal. Biochem. , 106 : 78 – 91 .
   PubMed Web of Science ®Google Scholar