311
Views
27
CrossRef citations to date
0
Altmetric
Research Article

Isolation of monocytes from whole blood by density gradient centrifugation and counter-current elutriation followed by cryopreservation: six years' experience

, , , &
Pages 357-366 | Published online: 08 Jul 2009

REFERENCES

  • Lorenz HM, Lagoo AS, Lagoo-Deenadalayan SA, Barber WH, Kalden JR, Hardy KJ. Epitopespeci ®c signaling through CD45 on T lymphocytes leads to cAMP synthesis in monocytes after ICAM-1-dependent cellular interaction. Eur J Immunol 1998; 28: 2300 ± 10.
  • Rosenfeld ME. Cellular mechanisms in the development of atherosclerosis. Diabetes Res Clin Pract 1996; 30 Suppl: 1 ± 11.
  • Ross R. Atherosclerosis ± an in¯ammatory disease. N Engl J Med 1999; 340: 115 ± 26.
  • Clauss M, Gerlach M, Gerlach H, Brett J, Wang F, Familletti PC, Pan YC, Olander JV, Connolly DT, Stern D. Vascular permeability factor: a tumor-derived polypeptide that induces endothelial cell and monocyte procoagulant activity, and promotes monocyte migration. J Exp Med 1990; 172: 1535 ± 45.
  • Jones BM, Nicholson JK, Holman RC, Hubbard M. Comparison of monocyte separation methods using ¯ow cytometric analysis. J Immunol Methods 1989; 125: 41 ± 7.
  • Rodenburg RJ, van den Hoogen FH, van de Putte LB, van Venrooij WJ. Peripheral blood monocytes of rheumatoid arthritis patients do not express elevated TNF alpha, IL-1beta, and IL-8 mRNA levels. A comparison of monocyte isolation procedures. J Immunol Methods 1998; 221: 169 ± 75.
  • Almici C, Carlo-Stella C, Donnenberg AD, Rizzoli V. Counter¯ow centrifugal elutriation: present and future. Bone Marrow Transpl 1993; 12: 105 ± 8.
  • Garner SF, Thomson AR, Lubenko A, Savage J. Monocyte isolation by ¯ow cytometer-monitored centrifugal elutriation: a preparative tool for antibody-dependent cell-mediated cytotoxicity (ADCC) assays. Br J Biomed Sci 1994; 51: 35 ± 43.
  • Faradji A, Bohbot A, Schmitt-Goguel M, Siffert JC, Dumont S, Wiesel ML, Piemont Y, Eischen A, Bergerat JP, Bartholeyns J. Large-scale isolation of human blood monocytes by continuous ¯ow centrifugation leukapheresis and counter¯ow centrifugation elutriation for adoptive cellular immunotherapy in cancer patients. J Immunol Methods 1994; 174: 297 ± 309.
  • Bernard J, Ittelet D, Christoph A, Potron G, Adjizian JC, Kochman S, Lopez M. Adherent-free generation of functional dendritic cells from puri®ed blood monocytes in view of potential clinical use. Hematol Cell Ther 1998; 40: 17 ± 26.
  • Zetterlund A, Eklund A, Hallden G, Hernbrandt R, Lundahl J. Elutriation of human alveolar macrophages: in¯uence on cell surface receptor expression. Scand J Clin Lab Invest 1998; 58: 29 ± 34.
  • Bùyum A. Isolation of mononuclear cells and granulocytes from human blood. Scand J Clin Lab Invest 1968; 21 Suppl 97: 77 ± 89.
  • Weiner RS, Shah VO. Puri®cation of human monocytes: isolation and collection of large numbers of peripheral blood monocytes. J Immunol Methods 1980; 36: 89 ± 97.
  • Osnes LT, Westvik AÊ B, Kierulf P. Procoagulant and pro®brinolytic activities of cryopreserved human monocytes. Thromb Res 1994; 76: 373 ± 83.
  • Wharton S, Riley P. The suppression of endogenous protein degradation by fractions of foetal calf serum: dialysed serum is less able to suppress degradation in aged cells. Cell Biochem Funct 1986; 4: 189 ± 95.
  • Brandtzaeg P, évstebù R, Kierulf P. Quantitative detection of bacterial lipopolysaccharides in clinical specimens. In: Pollard AJ, Maiden MCJ, editors. Meningococcal disease. Totowa, NJ, USA: Humana Press; 2000. In press
  • Roos D, de Boer M. Puri®cation and cryopreservation of phagocytes from human blood. Methods Enzymol 1986; 132: 225 ± 43.
  • Hulstaert F, Hannet I, Deneys V, Munhyeshuli V, Reichert T, De Bruyere M, Strauss K. Age-related changes in human blood lymphocyte subpopulations. II. Varying kinetics of percentage and absolute count measurements. Clin Immunol Immunopathol 1994; 70: 152 ± 8.
  • Mason RR, Weiner RS. Application of the Beckman JE6-B Elutriator System in the isolation of human monocyte subpopulations. Scand J Haematol 1985; 34: 5 ± 8.
  • Osnes L, Westvik Aring, évstebù R, Joù GshyB, Okkenhaug C, Kierulf P. Lipopolysaccharide activation of human monocytes mediated by CD14, results in a coordinated synthesis of tissue factor, TNF-a and IL-6. J Endotoxin Res 1995; 2: 27 ± 35.
  • Brandtzaeg P, Osnes L, Ovstebo R, Joù GB, Westvik AB, Kierulf P. Net in¯ammatory capacity of human septic shock plasma evaluated by a monocyte-based target cell assay: identi®cation of interleukin-10 as a major functional deactivator of human monocytes [published erratum appears in J Exp Med 1996 Nov 1; 184(5): 2075]. J Exp Med 1996; 184: 51 ± 60.
  • Stent G, Joù GshyB, Kierulf P, AÊ sjù B. Macrophage tropism: fact or ®ction? J Leukoc Biol 1997; 62: 4 ± 11.
  • Osnes LT, Westvik AB, Joo GB, Okkenhaug C, Kierulf P. Inhibition of IL-1 induced tissue factor (TF) synthesis and procoagulant activity (PCA) in puri®ed human monocytes by IL-4, IL-10 and IL- 13. Cytokine 1996; 8: 822 ± 7.
  • Osnes LT, Foss KB, Joo GB, Okkenhaug C, Westvik AB, Ovstebo R, Kierulf P. Acetylsalicylic acid and sodium salicylate inhibit LPS-induced NF-kappa B/c-Rel nuclear translocation, and synthesis of tissue factor (TF) and tumor necrosis factor alfa (TNF-alpha) in human monocytes. Thromb Haemost 1996; 76: 970 ± 6.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.