Advanced search
Publication Cover
Scandinavian Journal of Clinical and Laboratory Investigation Volume 82, 2022 - Issue 1
Submit an article Journal homepage
2,030
Views
8
CrossRef citations to date
0
Altmetric
Technical Note

On measurements of glucagon secretion in healthy, obese, and Roux-en-Y gastric bypass operated individuals using sandwich ELISA

Nicolai J. Wewer Albrechtsena Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark;b NNF Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark;c Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, DenmarkCorrespondence[email protected]
ORCID Iconhttps://orcid.org/0000-0003-4230-5753View further author information
ORCID Icon,
Sasha A. S. Kjeldsena Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark;b NNF Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark;c Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark;d Department of Endocrinology, Bispebjerg University Hospital, Bispebjerg, DenmarkView further author information
,
Nicole J. Jensend Department of Endocrinology, Bispebjerg University Hospital, Bispebjerg, DenmarkView further author information
,
Jørgen Rungbyd Department of Endocrinology, Bispebjerg University Hospital, Bispebjerg, DenmarkView further author information
,
Simon Veedfaldc Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, DenmarkView further author information
,
Kirstine N. Bojsen-Møllere Department of Endocrinology, Hvidovre Hospital, Hvidovre, DenmarkView further author information
,
Carsten Dirksene Department of Endocrinology, Hvidovre Hospital, Hvidovre, DenmarkView further author information
,
Christian Z. Jensene Department of Endocrinology, Hvidovre Hospital, Hvidovre, DenmarkView further author information
,
Christoffer Martinussene Department of Endocrinology, Hvidovre Hospital, Hvidovre, DenmarkORCID Iconhttps://orcid.org/0000-0002-7210-0807View further author information
ORCID Icon,
Sten Madsbade Department of Endocrinology, Hvidovre Hospital, Hvidovre, DenmarkView further author information
&
Jens J. Holstc Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark;f NNF Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, DenmarkORCID Iconhttps://orcid.org/0000-0001-6853-3805View further author information
ORCID Icon show all
Pages 75-83 | Received 05 Sep 2021, Accepted 06 Dec 2021, Published online: 22 Dec 2021

References

  • Albrechtsen NJW, Kuhre RE, Pedersen J, et al. The biology of glucagon and the consequences of hyperglucagonemia. Biomark Med. 2016;10(11):1141–1151.
  • Janah L, Kjeldsen S, Galsgaard KD, et al. Glucagon receptor signaling and glucagon resistance. Int J Mol Sci. 2019;20(13):3314.
  • Albrechtsen NJW. The glucose-mobilizing effect of glucagon at fasting is mediated by cyclic AMP. Am J Physiol Endocrinol Metab. 2021;321(4):E571–E574.
  • Holst JJ, Wewer Albrechtsen NJ. Methods and guidelines for measurement of glucagon in plasma. Int J Mol Sci. 2019;20(21).
  • Wewer Albrechtsen NJ, Kuhre RE, Hornburg D, et al. Circulating glucagon 1-61 regulates blood glucose by increasing insulin secretion and hepatic glucose production. Cell Rep. 2017;21(6):1452–1460.
  • Wewer Albrechtsen NJ, Hornburg D, Albrechtsen R, et al. Oxyntomodulin identified as a marker of type 2 diabetes and gastric bypass surgery by mass-spectrometry based profiling of human plasma. EBioMedicine. 2016;7:112–120.
  • Holst JJ. Evidence that enteroglucagon (II) is identical with the C-terminal sequence (residues 33-69) of glicentin. Biochem J. 1982;207(3):381–388.
  • Bak M, Albrechtsen Wewer N, Pedersen J, et al. Specificity and sensitivity of commercially available methods for the measurement of human glucagon, oxyntomodulin and glicentin; 2013.
  • Wewer Albrechtsen NJ, Hartmann B, Veedfald S, et al. Hyperglucagonaemia analysed by glucagon sandwich ELISA: nonspecific interference or truly elevated levels? Diabetologia. 2014;57(9):1919–1926.
  • Albrechtsen NJW, Kuhre RE, Windeløv JA, et al. Dynamics of glucagon secretion in mice and rats revealed using a validated sandwich ELISA for small sample volumes. Am J Physiol Endocrinol Metab. 2016;311(2):E302–E309.
  • Roberts GP, Kay RG, Howard J, et al. Gastrectomy with roux-en-Y reconstruction as a lean model of bariatric surgery. Surg Obes Relat Dis. 2018;14(5):562–568.
  • Jorsal T, Wewer Albrechtsen NJ, Christensen MM, et al. Investigating intestinal glucagon after Roux-en-Y gastric bypass surgery. J Clin Endocrinol Metab. 2019;104(12):6403–6416.
  • Jensen CZ, Bojsen-Møller KN, Svane MS, et al. Responses of gut and pancreatic hormones, bile acids, and fibroblast growth factor-21 differ to glucose, protein, and fat ingestion after gastric bypass surgery. Am J Physiol Gastrointest Liver Physiol. 2020;318(4):G661–g672.
  • Martinussen C, Veedfald S, Dirksen C, et al. The effect of acute dual SGLT1/SGLT2 inhibition on incretin release and glucose metabolism after gastric bypass surgery. Am J Physiol Endocrinol Metab. 2020;318(6):E956–E964.
  • Cegla J, Jones BJ, Howard J, et al. The preanalytical stability of glucagon as measured by liquid chromatography tandem mass spectrometry and two commercially available immunoassays. Ann Clin Biochem. 2017;54(2):293–296.
  • Wewer Albrechtsen NJ, Veedfald S, Plamboeck A, et al. Inability of some commercial assays to measure suppression of glucagon secretion. J Diabetes Res. 2016;2016:8352957.

Related research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.