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Scandinavian Journal of Clinical and Laboratory Investigation Volume 43, 1983 - Issue 7
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Original Article

Methods of reducing the effect of spontaneous dissociation of antigen-antibody interactions in radioimmunoassays with special reference to internal sample attenuator counting (ISAC)

H. Eriksson Department of Pure and Applied Biochemistry, Chemical Center, University of Lund, S-220 07, Lund, Sweden
,
I. Larsson Department of Nuclear Medicine, University of Lund at Malmö General Hospital, S-214 01, Malmö, Sweden
,
J. I. Thorell Department of Nuclear Medicine, University of Lund at Malmö General Hospital, S-214 01, Malmö, Sweden
&
B. Mattiasson Department of Pure and Applied Biochemistry, Chemical Center, University of Lund, S-220 07, Lund, Sweden
Pages 575-581 | Received 29 Dec 1982, Accepted 09 Jun 1983, Published online: 17 Mar 2010

References

  • Cameron E. H.D., Hillier S. G., Griffiths K. Steroid Immunoassay Proceedings of Vth Tenovus Workshop. Alpha Omega Alpha Publishing, Caerphilly 1975
  • Eriksson H., Mattiasson B., Thorell J. I. Use of an internal sample attenuator in radioimmunoassay. Assay of triiodothyronine (T3) using starch particles containing entrapped charcoal and bismuth oxide in combination with free antibodies. J. Immunol. Methods 1981; 42: 105
  • Gharib H., Ryan F. J., Mayberry W. E., Hockert T. Radioimmunoassay for triiodothyronine (T3): I. Affinity and specificity of the antibody for T3. J. clin. Endocr. Metab. 1971; 33: 509
  • Herbert V., Lau K-S., Gottlieb C. W., Bleicher S. J. Coated charcoal immunoassay of insulin. J. clin. Endocr. Metab. 1965; 25: 1375
  • Parker C. W. Radioimmunoassay of Biologically Active Compounds. Prentice-Hall Inc., New Jersey 1976; 160
  • Thorell J. I., Johansson B. G. Enzymatic iodination of polypeptides with 125I to high specific activity. Biochim. biophys. acta 1971; 251: 363
  • Thorell J. I., Larson S. M. Radioimmunoassay and Related Techniques. Mosby, St. Louis 1978; 116
  • Thorell J. I. Internal sample attenuator counting (ISAC). A new technique for separating and measuring bound and free activity in radioimmunoassays. Clin. Chem. 1981; 42: 105

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