The amino terminal portion of cerebrospinal fluid cystatin C in hereditary cystatin C amyloid angiopathy is not truncated: direct sequence analysis from agarose gel electropherograms

References

• Jensson O, Gudmundsson G, Arnason A, et al. Hereditary cystatin C (γ-trace) amyloid angiopathy of the CNS causing cerebral haemorrhage. Acta Neurol Scand 1987; 76: 102–24
   PubMed Web of Science ®Google Scholar
• Löfberg H, Grubb A O, Nilsson E K, et al. Immunohistochemical characterization of the amyloid deposits and quantitation of pertinent cerebrospinal fluid proteins in hereditary cerebral haemorrhage with amyloidosis. Stroke 1987; 18: 431–40
   PubMed Web of Science ®Google Scholar
• Thorsteinsson L, Blondal H, Jensson O, Gudmundsson G. Distribution of cystatin C amyloid deposits in the Icelandic patients with hereditary cystatin C amyloid angiopathy. Amyloid and Amyloidosis, T Isobe. Plenum Press, New York 1988; 585–90
   Google Scholar
• Grubb A, Jensson O, Gudmundsson G, Arnasson A, Löfberg H, Malm J. Abnormal metabolism of gamma trace alkaline microprotein. Basic defect in hereditary cerebral haemorrhage with amyloidosis. N Engl J Med 1984; 311: 1547–9
   PubMed Web of Science ®Google Scholar
• Cohen D H, Feiner H, Jensson O, Frangione B. Amyloid fibril in hereditary cerebral haemorrhage with amyloidosis (HCHWA) is related to the gastroenteropancreatic neuroendocrine protein gamma trace. J Exp Med 1983; 158: 623–8
   PubMed Web of Science ®Google Scholar
• Ghiso J, Jensson O, Frangione B. Amyloid fibrils in hereditary cerebral haemorrhage with amyloidosis of Icelandic type is a variant of y-trace basic protein (cystatin C). Proc Natl Acad Sci USA 1986; 83: 2974–8
   PubMed Web of Science ®Google Scholar
• Palsdottir A, Abrahamson M, Thorsteinsson L, et al. Mutation in the cystatin C gene causes hereditary brain haemorrhage. Lancet 1988; ii: 603–4
   Google Scholar
• Löfberg H, Grubb A O. Quantitation of y-trace in human biological fluids: indication for production in the central nervous system. Scand J Clin Lab Invest 1979; 39: 619–26
   PubMed Web of Science ®Google Scholar
• Abrahamson M, Barrett A J, Salvesen G, Grubb A. Isolation of six cysteine proteinase inhibitors from human urine. Their physiochemical and enzyme kinetic properties and concentrations in biological fluids. J Biol Chem 1986; 261: 11282–9
   PubMed Web of Science ®Google Scholar
• Löfberg H, Grubb A O, Brun A. Human brain cortical neurons contain γ-trace. Rapid isolation, immunohistochemical and physiochemical characterization of human γ-trace. Biomed Res 1981; 2: 298–306
   Web of Science ®Google Scholar
• Abrahamson M, Dalbøge H, Olafsson I, Carlsen S, Grubb A. Effficient production of native, biologically active human cystatin C by Escherichia coli. FEBS Lett 1988; 236: 14–8
   PubMed Web of Science ®Google Scholar
• Abrahamson M, Olafsson I, Grubb A, (1989) An efficient E. coli expression system for human cystatin C. Production and characterization of the native and N-terminally Ala-Met-Glu-Ala-Glu-extended inhibitor. Proceedings of the 7th Meeting of International Committee on Proteolysis (ICOP), ShimodaJapan, May, 16–201988, E Katunuma, et al. Springer-Verlag, Berlin, in press
   Google Scholar
• Abrahamson M, Ritonja A, Brown M A, Grubb A, Machleidt W, Barrett A J. Identification of the probable inhibitory reactive sites of the cysteine proteinase inhibitors human cystatin C and chicken cystatin. J Biol Chem 1987; 262: 9688–94
   PubMed Web of Science ®Google Scholar
• Blumberg S, Schechter I, Berger A. The purification of papain by affinity chromatography. Eur J Biochem 1970; 15: 97–102
   PubMedGoogle Scholar
• Glazer A N, Smith E L. Phenolic hydroxyl ionization in papain. J Biol Chem 1961; 236: 2948–51
   PubMed Web of Science ®Google Scholar
• Barrett A J, Kembhavi A A, Brown M A, et al. L-trans-Epoxysuccinyl-leucylamido(4-guanidino) butane (E-64) and its analogues as inhibitors of cysteine proteinases including cathepsins B, H and L. Biochem J 1982; 201: 189–98
   PubMed Web of Science ®Google Scholar
• Barrett A J. a2-Macroglobulin. Methods Enzymol 1981; 80: 737–54
   PubMed Web of Science ®Google Scholar
• van Der Giessen M, De Lange B, van Der Lee B N. The production of precipitating antiglobulin reagents specific for the subclasses of human IgG. Immunology 1974; 27: 655–63
   PubMed Web of Science ®Google Scholar
• Thorell J I, Johansson B G. Enzymatic iodination of polypeptides with l25I to high specific activity. Biochim Biophys Acta 1971; 251: 363–9
   PubMed Web of Science ®Google Scholar
• Glynn P, Gilbert H, Newcombe Cuzner M L. Rapid analysis of immunoglobulin isoelectric focusing patterns with cellulose nitrate sheets and immunoperoxidase staining. J Immunol Methods 1982; 51: 251–7
   PubMed Web of Science ®Google Scholar
• Jeppson J O, Laurell C B, Franzen B. Agarose gel electrophoresis. Clin Chem 1979; 25: 629–38
   PubMed Web of Science ®Google Scholar
• Hewick R M, Hunkapiller M W, Hood L E, Dreyer W. A gas-liquid solid-phase peptide and protein sequenator. J Biol Chem 1981; 256: 7990–7
   PubMed Web of Science ®Google Scholar
• Barrett A J, Kirschke H, Cathepsin B, Cathepsin H, Cathepsin L. Methods Enzymol 1981; 80: 535–61
   PubMed Web of Science ®Google Scholar
• Laurell C B. Electroimmunoassay. Scand J Clin Lab Invest 1972; 29(Suppl. 124)21–37
   Web of Science ®Google Scholar
• Grubb A, Löfberg H. Human y-trace, a basic microprotein: Amino acid sequence and presence in the adenophypophysis. Proc Natl Acad Sci USA 1982; 79: 3024–7
   PubMed Web of Science ®Google Scholar
• Abrahamson M, Grubb A, Olafsson I, Lundwall Å. Molecular cloning and sequence analysis of cDNA coding for the precursor of the human cysteine proteinase inhibitor cystatin C. FEBS Lett 1987; 216: 229–33
   PubMed Web of Science ®Google Scholar
• Matsudaira P. Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. J Biol Chem 1987; 262: 10035–8
   PubMed Web of Science ®Google Scholar
• Glenner G G. Amyloid deposits and amyloidosis. The β-fibrllosis. N Engl J Med 1980; 302: 1283–92, 133–43
   PubMed Web of Science ®Google Scholar