11
Views
1
CrossRef citations to date
0
Altmetric
Research Article

A modified fast micro method in agarose for isotype, allotype, light chain and idiotype-specific analysis of antibody clonotypes to bacterial virulence antigens

, , , , &
Pages 661-668 | Published online: 08 Jul 2009

REFERENCES

  • Righetti PG, Gianazza E, Gelfi C, Chiari M. Iso-electric focusing. In: Hames BD, Rickwood D, edi-tors. Gel electrophoresis of proteins: a practical approach. Oxford: IRL Press, 1990: 149 — 215.
  • Shibeci A, Wade AW, Depew WT, Szewczuk MR. Analysis of serum antibody repertoires by isoelec-tric focusing and capillary blotting onto nitrocellu-lose paper. J Immunol Methods 1986; 89: 201 — 5.
  • Williamson AR. Isoelectric focusing of immuno-globulins. In: Weir DM, editor. Handbook of experimental immunology, 2nd edn. Oxford: Black-well, 1973; 8.1 — 8.23
  • D'Amelio R, Del Giudice G, De Rosa F, Brighouse G, Teggi, Lambert PH. Spectrotypic analysis of humoral response in human hydatidosis. Clin Exp Immunol 1986; 76: 117 — 20.
  • Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applica-tions. Proc Natl Acad Sci 1979; 76: 4350 — 4.
  • Reinhart MP, Malamud D. Protein transfer from isoelectric focusing gels: the native blot. Anal Bio-chem 1982; 123: 229 — 35.
  • Dorries R, Meulen VT. Detection and identifica-tion of virus-specific, oligoclonal IgG in unconcen-trated cerebrospinal fluid by immunoblot technique. J Neuroimmunol 1984; 7: 77 — 89.
  • Slade HB, Pica RV, Pahwa SG. Detection of HIV-specific antibodies in infancy by isoelectric focusing and affinity immunoblotting. J Infect Dis 1989; 160: 126 — 30.
  • Cruz M, Hansen K, Ernerudh J, Steere AC, Link H. Lyme arthritis: oligoclonal anti-Borrelia burg-dorferi IgG antibodies occur in joint fluid and serum. Scand J Immunol 1991; 33: 61–71.
  • McLahan R. Monoclonal immunoglobulins: affi-nity-blotting for low concentrations in serum. Clin Chem 1989; 35: 478 — 81.
  • Dorries R, Kaiser R, Meulen VT. Human immuno-deficiency virus infection: affinity-mediated immu-noblot detects intrathecal synthesis of oligoclonal IgG specific for individual viral proteins. AIDS Res Human Retroviruses 1989; 5: 303 — 10.
  • Hamilton RG, Reimer CB, Rodkey LS. Quality control of murine monoclonal antibodies using iso-electric focusing affinity immunoblot analysis. Hybridoma 1987; 6: 205 — 17.
  • Hamilton RG, Roebber M, Reimer CB, Rodkey LS. Isoelectric focusing-affinity immunoblot analy-sis of mouse monoclonal antibodies to the four human IgG subclasses. Electrophoresis 1987; 8: 127 — 34.
  • Adderson EE, Johnston JM, Schackelford PG, Carrol WI. Development of the human antibody repertoire. Pediatr Res 1992; 32: 257 — 63.
  • Jefferis R, et al. Evaluation of monoclonal antibo-dies having specificity for human IgG subclasses: results of the second IUIS /WHO collaborative study. Immun Lett 1992; 31: 143 — 68.
  • Gustafsson B, Whitmore E, Tiru M. Neutralization of tetanus toxin by human monoclonal antibodies directed against tetanus toxin and fragment C. hybridoma 1993; 12: 699 — 708.
  • Wybo I, Van Blerk M, Malfait R, Goubert P, Gorus F. Oligoclonal bands in cerebrospinal fluid detected by PhastSystem isoelectric focusing. Clin Chem 1990; 36: 123 — 5.
  • Olsson I, Axio-Fredriksson UB, Degerman M, Olsson B. Fast horizontal electrophoresis. I. Iso-electric focusing and polyacrylamide gel electro-phoresis using PhastSystem. Electrophoresis 1988; 9: 16–22.
  • Vartdal F, Vandvik B. Imprint immunofixation of electrofocused immunoglobulins. J Immunol Methods 1983; 62: 23–9.
  • Jefferis R. Polyclonal and monoclonal antibody reagents specific for IgG subclasses. Monogr Allergy 1986; 19: 71 — 85.
  • Jefferis R. Human IgG subclass-specific epitopes recognised by murine monoclonal antibodies. Monogr Allergy 1986; 20: 26— 33.
  • Jefferis R, et al. Evaluation of monoclonal antibo-dies having specificity for human IgG subclasses: results of an IUIS /WHO collaborative study. Immunol Lett 1985; 10: 223 — 52.
  • Lucas AH, Langley RJ, Granoff DM, Nahm MH, Kitamura MY, Scott MG. An idiotypic marker associated with a germline encoded lc light chain variable region that predominates the vaccine-induced human antibody response to the Haemo-philus influenzae b polysaccharide. J Clin Invest 1991; 88: 1811–8.
  • Nagao AT, Martinez CC, Vieira VS, Takano AO, Costa-Carvalho BT, Carneiro-Sampaio MMS. Pla-cental transfer of IgG and IgG subclass antibodies anti-purified Escherichia coli LPS 016, 06 and 0111. Scand J Immunol 1998; 47: 609 — 14.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.