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Article

Real‐time PCR of host DNA in feces to study differential exfoliation of colonocytes between rats and humans

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Pages 852-857 | Received 15 Dec 2003, Accepted 01 Apr 2004, Published online: 08 Jul 2009

References

  • Lipkin M, Bell B, Sherlock P. Cell proliferation kinetics in the gastrointestinal tract of man. I. Cell renewal in colon and rectum. J Clin Invest 1963;42:767–76.
  • Lipkin M. Biomarkers of increased susceptibility to gastrointestinal cancer: new application to studies of cancer prevention in human subjects. Cancer Res 1988;48:235–45.
  • Hall PA, Coates PJ, Ansari B, Hopwood D. Regulation of cell number in the mammalian gastrointestinal tract: the importance of apoptosis. J Cell Sci 1994;107:3569–77.
  • Pink IJ, Croft DN, Creamer B. Cell loss from small intestinal mucosa: a morphological study. Gut 1970;11:217–22.
  • Cheng H, Leblond CP. Origin, differentiation and renewal of the four main epithelial cell types in the mouse small intestine. I. Columnar cells. Am J Anat 1974;141:461–79.
  • Leblond CP. The life history of cells in renewing systems. Am J Anat 1981;160:114–58.
  • Warwick R, Williams PL. Gray’s Anatomy, 35th ed. London: Longman; 1973. p. 1272–3.
  • Junquiera LC, Carneiro J, Kelo RO. Basic Histology, 6th ed. Los Altos, CA, USA: Lange Medical Publication159–66.
  • Alberts B, Bray D, Lewis J, Raff M, Roberts K, Watson J. Molecular biology of the cell, 3rd ed. New York: Garland Publishing; 1994. p. 1155.
  • Baron DA, Miller DH. Extrusion of colonic epithelial cells in vitro. J Electron Microscop Tech 1990;16:15–24.
  • Cheng H, Leblond CP. Origin, differentiation and renewal of the four main epithelial cell types in the mouse small intestine. V. Unitarian theory of the origin of the four epithelial cell types. Am J Anat 1974;141:537–61.
  • Tsubouchi S, Leblond CP. Migration and turnover of entero-endocrine and caveolated cells in the epithelium of the descending colon, as shown by radioautography after continuous infusion of 3H-thymidine into mice. Am J Anat 1979; 156:43151.
  • Ferreira MN, Leblond CP. Argentaffin and other ‘endocrine’ cells of the small intestine in the adult mouse. II. Renewal. Am J Anat 1971;131:331–9.
  • Yeomans ND, Trier JS. Epithelial cell proliferation and migration in the developing rat gastric mucosa. Dev Biol 1976; 53:206–16.
  • Sato M, Ahnen DJ. Regional variability of colonocyte growth and differentiation in the rat. Anat Rec 1992;223:409–14.
  • Barkla DH, Gibson PR. The fate of epithelial cells in the human large intestine. Pathology 1999;31:230–8.
  • Strater J, Koretz K, Gunthert AR, Moller P. In situ detection of enterocytic apoptosis in normal colonic mucosa and in familial adenomatous polyposis. Gut 1995;37:819–25.
  • Sesink AL, Termont DS, Kleibeuker JH, Van der Meer R. Red meat and colon cancer: dietary haem-induced colonic cytotoxicity and epithelial hyperproliferation are inhibited by calcium. Carcinogenesis 2001;22:1653–9.
  • Cats A, Kleibeuker JH, Van der Meer R, Kuipers F, Sluiter WJ, Hardonk MJ, et al. Randomized, double-blind, placebo-controlled intervention study with supplemental calcium in families with hereditary non-polyposis colorectal cancer. J Natl Cancer Inst 1995;87:598–603.
  • Van der Meer R, Lapré JA, Govers MJ, Kleibeuker JH. Mechanisms of the intestinal effects of dietary fats and milk products on colon carcinogenesis. Cancer Lett 1997;114:75–83.
  • Iyengar V, Albaugh GP, Lohani A, Nair PP. Human stools as a source of viable colonic epithelial cells. Faseb J 1991;5:2856–9.
  • Albaugh GP, Iyengar V, Lohani A, Malayeri M, Bala S, Nair PP. Isolation of exfoliated colonic epithelial cells, a novel, noninvasive approach to the study of cellular markers. Int J Cancer 1992;52:347–50.
  • Poinar HN, Hofreiter M, Spaulding WG, Martin PS, Stankiewicz BA, Bland H, et al. Molecular coproscopy: dung and diet of the extinct ground sloth Nothrotheriops shastensis. Science 1998; 281:402–6.
  • Sesink AL, Termont DS, Kleibeuker JH, Van der Meer R. Red meat and colon cancer: the cytotoxic and hyperproliferative effects of dietary heme. Cancer Res 1999;59:5704–9.
  • Govers MJ, Termont DS, Lapré JA, Kleibeuker JH, Vonk RJ, Van der Meer R. Calcium in milk products precipitates intestinal fatty acids and secondary bile acids and thus inhibits colonic cytotoxicity in humans. Cancer Res 1996;56:3270–5.
  • Villa E, Dugani A, Rebecchi AM, Vignoli A, Grottola A, Buttafoco P, et al. Identification of subjects at risk for colorectal carcinoma through a test based on K-ras determination in the stool. Gastroenterology 1996;110:1346–53.
  • Klaassen CHW, Jeunink MAF, Prinsen CFM, Ruers TJM, Tan ACITL, Strobbe JCA, et al. Quantification of human DNA in feces as a diagnostic test for the presence of colorectal cancer. Clin Chem 2003;49:1185–7.
  • Antschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, et al. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acid Res 1997;25:3389–402.
  • Al-Soud AW, Radstrom P. Capacity of nine thermostable DNA polymerases to mediate DNA amplification in the presence of PCR-inhibiting samples. Appl Environ Microbiol 1998;64:3748–53.
  • Ahlquist DA, Harrington JJ, Burgert LJ, Roche PC. Morphometric analysis of the ‘mucocellular layer’ overlying colorectal cancer and normal mucosa: relevance to exfoliation and stool screening. Hum Pathol 2000;31:51–7.
  • Losi L, Benhattar J, Costa J. Stability of K-ras mutations throughout the natural history of human colorectal cancer. Eur J Cancer 1992;28A:1115–20.
  • Benhattar J, Losi L, Chaubert P, Givel JC, Costa J. Prognostic significance of K-ras mutations in colorectal carcinoma. Gastroenterology 1993;104:1044–8.
  • Caldas C, Hahn SA, Hruban RH, Redston MS, Yeo CJ, Kern SE. Detection of K-ras mutations in the stool of patients with pancreatic adenocarcinoma and pancreatic ductal hyperplasia. Cancer Res 1994;54:3568–73.
  • Dutta SK, Silverstein RJ, Fleisher AS, Vengunrlekar SM, Shami S. Increased expression of CD44 in isolated fecal colonocytes in patients with colonic neoplasia. Gastroenterology 1995; 108 Suppl:A463.
  • Ahlquist DA, Skoletsky JE, Boynton KA, Harrington JJ, Mahoney DW, Pierceall WE, et al. Colorectal cancer screening by detection of altered human DNA in stool: feasibility of a multitarget assay panel. Gastroenterology 2000;119:1219–27.
  • Traverso G, Shuber A, Levin B, Johnson C, OlssonL, Schoetz DJ Jr, et al. Detection of APC mutations in fecal DNA from patients with colorectal tumors. N Engl J Med 2002;346:311–20.

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